Molecular characterization of Vibrio harveyi bacteriophages isolated from aquaculture environments along the coast of India.

Seven bacteriophages specific to Vibrio harveyi, the causative agent of luminous vibriosis in shrimp, were isolated from coastal aquaculture systems like shrimp farms, hatcheries and tidal creeks along the east and west coast of India. All the seven phages were found to have the typical head and tail morphology with double-stranded DNA as genetic material. Morphologically, six phages were grouped under family Siphoviridae and one under Myoviridae. These phages were further characterized with respect to host range, morphology and structural proteins. Genomic fingerprinting was carried out using restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD). Major capsid proteins of all the phages detected by SDS-PAGE were distinct from one another. All the phages were found to be highly lytic for V. harveyi and had different lytic spectrum for the large number of isolates tested. Six of the seven phages isolated had a broad lytic spectrum and could be potential candidates for biocontrol of V. harveyi in aquaculture systems.

[1]  I. Karunasagar,et al.  Biocontrol of pathogens in shrimp hatcheries using bacteriophages , 2007 .

[2]  I. Karunasagar,et al.  Isolation of Vibrio harveyi bacteriophage with a potential for biocontrol of luminous vibriosis in hatchery environments , 2006 .

[3]  T. Flegel,et al.  Partial characterization of a novel bacteriophage of Vibrio harveyi isolated from shrimp culture ponds in Thailand. , 2005, Virus research.

[4]  O. White,et al.  Complete Genome Sequence of the Broad-Host-Range Vibriophage KVP40: Comparative Genomics of a T4-Related Bacteriophage , 2003, Journal of bacteriology.

[5]  I. Connerton,et al.  Isolation and Characterization of Campylobacter Bacteriophages from Retail Poultry , 2003, Applied and Environmental Microbiology.

[6]  J. Oakey,et al.  Experimental bacteriophage-mediated virulence in strains of Vibrio harveyi. , 2003, Diseases of aquatic organisms.

[7]  J. Gill,et al.  Bacteriophages of Erwinia amylovora , 2003, Applied and Environmental Microbiology.

[8]  L. Owens,et al.  The complete nucleotide sequence of the Vibrio harveyi bacteriophage VHML , 2002, Journal of applied microbiology.

[9]  Angelo DePaola,et al.  Phage Therapy of Local and Systemic Disease Caused by Vibrio vulnificus in Iron-Dextran-Treated Mice , 2002, Infection and Immunity.

[10]  L. Owens,et al.  A new bacteriophage, VHML, isolated from a toxin‐producing strain of Vibrio harveyi in tropical Australia , 2000, Journal of applied microbiology.

[11]  M. Skurnik,et al.  Bacteriophage φYeO3-12, Specific forYersinia enterocolitica Serotype O:3, Is Related to Coliphages T3 and T7 , 2000, Journal of bacteriology.

[12]  T. Nakai,et al.  Isolation of Bacteriophages Specific to a Fish Pathogen, Pseudomonas plecoglossicida, as a Candidate for Disease Control , 2000, Applied and Environmental Microbiology.

[13]  T. Nakai,et al.  Protective effects of bacteriophage on experimental Lactococcus garvieae infection in yellowtail. , 1999, Diseases of aquatic organisms.

[14]  J. Fuhrman.  Marine viruses and their biogeochemical and ecological effects , 1999, Nature.

[15]  T. Flegel,et al.  Lethal toxicity of Vibrio harveyi to cultivated Penaeus monodon induced by a bacteriophage , 1999 .

[16]  H. Ackermann.  Tailed Bacteriophages: The Order Caudovirales , 1998, Advances in Virus Research.

[17]  Y. Li,et al.  Vibrios associated with Penaeus chinensis (Crustacea: Decapoda) larvae in Chinese shrimp hatcheries , 1998 .

[18]  R. Bodmer,et al.  Large- and small-scale preparation of bacteriophage λ lysate and DNA , 1998 .

[19]  Alvarez,et al.  Vibrio harveyi: a pathogen of penaeid shrimps and fish in Venezuela. , 1998, Journal of fish diseases.

[20]  M. Lovell,et al.  Use of Lytic Bacteriophage for Control of Experimental Escherichia coli Septicemia and Meningitis in Chickens and Calves , 1998, Clinical Diagnostic Laboratory Immunology.

[21]  D. Wareing,et al.  Characterisation of 16 Campylobacter jejuni and C. coli typing bacteriophages. , 1998, Journal of medical microbiology.

[22]  B. Rieland,et al.  Prevalence of Broad-Host-Range Lytic Bacteriophages of Sphaerotilus natans, Escherichia coli, andPseudomonas aeruginosa , 1998, Applied and Environmental Microbiology.

[23]  A. M. Chan,et al.  Phages Infecting Vibrio vulnificus Are Abundant and Diverse in Oysters (Crassostrea virginica) Collected from the Gulf of Mexico , 1998, Applied and Environmental Microbiology.

[24]  P. Barrow,et al.  Bacteriophage therapy and prophylaxis: rediscovery and renewed assessment of potential. , 1997, Trends in microbiology.

[25]  A. Blanch,et al.  Isolation of Vibrio harveyi from an ocular lesion in the short sunfish (Mola mola) , 1997 .

[26]  K. Muroga,et al.  Taxonomical re-examination of two pathogenic Vibrio species isolated from milkfish and swimming crab. , 1997 .

[27]  G. Kou,et al.  News & Notes: Isolation of Vibrio harveyi from Diseased Kuruma Prawns Penaeus japonicus , 1996, Current Microbiology.

[28]  M. Saeed.  ASSOCIATION OF VIBRIO HARVEYI WITH MORTALITIES IN CULTURED MARINE FISH IN KUWAIT , 1995 .

[29]  S. Ahrné,et al.  Randomly amplified polymorphic DNA (RAPD) for rapid typing of Lactobacillus plantarum strains , 1995, Letters in applied microbiology.

[30]  M. Pizzutto,et al.  Classification of isolates of Vibrio harveyi virulent to Penaeus monodon larvae by protein profile analysis and M13 DNA fingerprinting , 1995 .

[31]  I. Karunasagar,et al.  Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection , 1994 .

[32]  T. Miyazaki,et al.  Histopathology, Biochemistry, and Pathogenicity of Vibrio harveyi Infecting Black Tiger Prawn Penaeus monodon , 1994 .

[33]  T. Klaenhammer,et al.  Restriction/Modification Systems and Restriction Endonucleases Are More Effective on Lactococcal Bacteriophages That Have Emerged Recently in the Dairy Industry , 1993, Applied and environmental microbiology.

[34]  S. Matsuzaki,et al.  A Broad‐Host‐Range Vibriophage, KVP40, Isolated from Sea Water , 1992, Microbiology and immunology.

[35]  H. Matsumoto,et al.  Two Generalized Transducing Phages in Vibrio parahaemolyticus and Vibrio alginolyticus , 1991, Microbiology and immunology.

[36]  D. Lim,et al.  Vibrio harveyi, an opportunistic pathogen of common snook, Centropomus undecimalis (Bloch), held in captivity , 1990 .

[37]  F. Prevots,et al.  Taxonomic differentiation of 101 lactococcal bacteriophages and characterization of bacteriophages with unusually large genomes , 1990, Applied and environmental microbiology.

[38]  F. Brown,et al.  Virology: Directory & Dictionary of Animal Bacterial and Plant Viruses , 1989 .

[39]  G. Bratbak,et al.  High abundance of viruses found in aquatic environments , 1989, Nature.

[40]  T. Kawata,et al.  Structure of a Novel Bacteriophage VP3 for Vibrio parahaemolyticus , 1981, Microbiology and immunology.

[41]  Jen-Leih Wu,et al.  Biological Control of Fish Bacterial Pathogen,Aeromonas hydrophila,by Bacteriophage AH1 (魚病に関する国際セミナ-) , 1981 .

[42]  D. Duckworth,et al.  Inhibition of bacteriophage replication by extrachromosomal genetic elements. , 1981, Microbiological reviews.

[43]  R. Y. Morita,et al.  Incidence of Vibrio parahaemolyticus Bacteriophages and Other Vibrio Bacteriophages in Marine Samples , 1978, Applied and environmental microbiology.

[44]  K. Hantke.  Major outer membrane proteins of E. coli K12 serve as receptors for the phages T2 (protein Ia) and 434 (protein Ib) , 1978, Molecular and General Genetics MGG.

[45]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[46]  H. Nakanishi.  Isolation and properties of bacteriophages of Vibrio parahaemolyticus , 1966 .

[47]  B. Austin,et al.  Association of a bacteriophage with virulence in Vibrio harveyi. , 2003, Journal of fish diseases.

[48]  T. Nakai,et al.  Bacteriophage therapy of infectious diseases in aquaculture. , 2002, Research in microbiology.

[49]  K. Iczkowski,et al.  Bacteriophages show promise as antimicrobial agents. , 1998, The Journal of infection.

[50]  H. Ackermann,et al.  Classification of Vibrio bacteriophages. , 1984, Intervirology.

[51]  J. Crosse,et al.  A Method for isolating Pseudomonas mors-prunorum Phages from the Soil , 1958, Nature.