Microbial Composition Analysis for TCE Biostimulation and Bioremediation

Various molecular tools including real-time PCR, clone library, and automated ribosomal intergenic spacer analysis were used in this column study to analyze the change of microbial composition in response to the addition of various carbon donors and microbial inocula. Whey, emulsified oil and emulsified oil plus surfactant were used as carbon donors. According to the competitive electron accepting processes observed in the columns, four microbial groups were selected as targets in this study: dechlorinating bacteria, iron-reducing bacteria, sulfate reducing bacteria, and methanogens. The combination of the molecular tools developed in this study is proved to be able to effectively detect key microbial groups, and determine and track the microbial composition and its changes during the bioremediation of chlorinated compounds.

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