Biomass nutrient profiles of the microalga Nannochloropsis.

The nutritional composition of the marine eustigmatophyte Nannochloropsis spp. cultured in an indoor chemostat under continuous illumination was analyzed. Proximate composition, (moisture, ash, crude protein, available carbohydrates, fiber, lipids, and energy), nitrate, nucleic acid, mineral element (Na, K, Ca, Mg, Fe, Cu, Zn, Mn, Pb, Cd, Cr, Ni, Co, and S), fatty acid, and pigment (carotenoids and chlorophyll) concentrations were determined. On average, the biomass contained 37.6% (w/w) available carbohydrates, 28.8% crude protein, and 18.4% total lipids. Mineral in 100 g of dry biomass were as follows: Ca (972 mg), K (533 mg), Na (659 mg), Mg (316 mg), Zn (103 mg), Fe (136 mg), Mn (3.4 mg), Cu (35.0 mg), Ni (0.22 mg), and Co (<0.1 mg). Toxic heavy metal contents (Cd and Pb) were negligible. Fatty acid content was as follows (on percent dry weight): 0.6% of 14:0, 5.0% of 16:0; 4.7% of 16:1omega7, 3.8% of 18:1omega9, 0.4% of 18:2omega6; 0.7% of 20:4omega6, and 2.2% of 20:5omega3. Nutrient composition of the biomass was highly influenced by residence time in the photobioreactor. The biomass harvested for short residence times was richer in protein and eicosapentaenoic acid than biomass harvested for high residence time.

[1]  N. J. Antia,et al.  The keto-carotenoids of two marine coccoid members of the Eustigmatophyceae , 1982 .

[2]  R. Guillard,et al.  Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. , 1962, Canadian journal of microbiology.

[3]  Michael A. Borowitzka,et al.  Light and nitrogen deficiency effects on the growth and composition ofPhaeodactylum tricornutum , 1993 .

[4]  J. Pérez,et al.  Biomass nutrient profiles of the microalga Porphyridium cruentum , 2000 .

[5]  Takashi Kato,et al.  Production of Eicosapentaenoic Acid by a Marine Microalgae and Its Commercial Utilization for Aquaculture , 1992 .

[6]  Mª E. Torija Asasa.  Principios inmediatos y elementos minerales en hongos comestibles , 1980 .

[7]  R. Andersen,et al.  Ultrastructure, pigment composition, and 18S rRNA gene sequence for Nannochloropsis granulata sp. nov. (Monodopsidaceae, Eustigmatophyceae), a marine ultraplankter isolated from the Skagerrak, northeast Atlantic Ocean , 1996 .

[8]  José Luis Guil-Guerrero,et al.  BIOMASS NUTRIENT PROFILES OF THE MICROALGA PHAEODACTYLUM TRICORNUTUM , 2001 .

[9]  T. Nakamura,et al.  Identification of the alga known as marine chlorella as a member of the eustigmatophyceae , 1986 .

[10]  P. Pohl,et al.  Formation of biomass, total protein, chlorophylls, lipids and fatty acids in green and blue-green algae during one growth phase.☆ , 1984 .

[11]  Y. Carmeli,et al.  REGULATION OF FATTY ACID COMPOSITION BY IRRADIANCE LEVEL IN THE EUSTIGMATOPHYTE NANNOCHLOROPSIS SP. 1 , 1989 .

[12]  G. Lepage,et al.  Improved recovery of fatty acid through direct transesterification without prior extraction or purification. , 1984, Journal of lipid research.

[13]  P. Koivistoinen,et al.  A method for sequential quantitative separation and determination of protein, RNA, DNA, lipid, and glycogen from a single rat liver homogenate or from a subcellular fraction. , 1967, Analytical biochemistry.

[14]  P. Roessler,et al.  RADIOLABELING STUDIES OF LIPIDS AND FATTY ACIDS IN NANNOCHLOROPSIS (EUSTIGMATOPHYCEAE), AN OLEAGINOUS MARINE ALGA 1 , 1994 .

[15]  D. Hibberd.  Notes on the taxonomy and nomenclature of the algal classes Eustigmatophyceae and Tribophyceae (synonym Xanthophyceae) , 1981 .

[16]  D. Osborne,et al.  Análisis de los nutrientes de los alimentos , 1985 .

[17]  M. Kates,et al.  Lipid components of diatoms. , 1966, Biochimica et biophysica acta.

[18]  S. J. Whittle,et al.  The chloroplast pigments of the algal classes Eustigmatophyceae and Xanthophyceae. I. Eustigmatophyceae , 1975 .

[19]  Paul Geladi,et al.  Principal Component Analysis , 1987, Comprehensive Chemometrics.

[20]  W. Darley.  Algal Biology: A Physiological Approach , 1982 .

[21]  I. Jolliffe.  Principal Component Analysis , 2002 .

[22]  G. C. Zittelli,et al.  Production of eicosapentaenoic acid by Nannochloropsis sp. cultures in outdoor tubular photobioreactors , 1999 .

[23]  J. Dyerberg.  Linolenate-derived polyunsaturated fatty acids and prevention of atherosclerosis. , 2009, Nutrition reviews.

[24]  Malcolm R. Brown,et al.  THE BIOCHEMICAL COMPOSITION OF MARINE MICROALGAE FROM THE CLASS EUSTIGMATOPHYCEAE 1 , 1993 .

[25]  J. Whyte.  Biochemical composition and energy content of six species of phytoplankton used in mariculture of bivalves , 1987 .

[26]  Malcolm R. Brown,et al.  The amino-acid and sugar composition of 16 species of microalgae used in mariculture , 1991 .

[27]  J. Abalde,et al.  Biomass production and biochemical composition in mass cultures of the marine microalga Isochrysis galbana Parke at varying nutrient concentrations , 1986 .

[28]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[29]  E. Becker.  Microalgae: Biotechnology and Microbiology , 1994 .

[30]  Z. Cohen,et al.  Microalgae as a source of omega 3 fatty acids. , 1995, World review of nutrition and dietetics.

[31]  Y. Carmeli,et al.  Biochemical quality of marine unicellular algae with special emphasis on lipid composition. II: Nannochloropsis sp. , 1993 .