Meeting Report: Fungal ITS Workshop (October 2012)

This report summarizes a meeting held in Boulder, CO USA (19–20 October 2012) on fungal community analyses using ultra-high-throughput sequencing of the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA (rRNA) genes. The meeting was organized as a two-day workshop, with the primary goal of supporting collaboration among researchers for improving fungal ITS sequence resources and developing recommendations for standard ITS primers for the research community.

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[2]  John L. Spouge,et al.  Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi , 2012, Proceedings of the National Academy of Sciences.

[3]  R. Henrik Nilsson,et al.  Progress in molecular and morphological taxon discovery in Fungi and options for formal classification of environmental sequences , 2011 .

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[5]  James R. Cole,et al.  The Ribosomal Database Project: improved alignments and new tools for rRNA analysis , 2008, Nucleic Acids Res..

[6]  Kuan-Liang Liu,et al.  Accurate, Rapid Taxonomic Classification of Fungal Large-Subunit rRNA Genes , 2011, Applied and Environmental Microbiology.

[7]  W. Ludwig,et al.  SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB , 2007, Nucleic acids research.

[8]  D. Hibbett,et al.  Evolutionary relationships within the fungi: analyses of nuclear small subunit rRNA sequences. , 1992, Molecular phylogenetics and evolution.

[9]  William A. Walters,et al.  Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms , 2012, The ISME Journal.

[10]  Alexander Keller,et al.  The ITS2 Database III—sequences and structures for phylogeny , 2009, Nucleic Acids Res..

[11]  Eric P. Nawrocki,et al.  An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea , 2011, The ISME Journal.

[12]  Thomas D. Bruns,et al.  Fungal Community Ecology: A Hybrid Beast with a Molecular Master , 2008 .

[13]  Michael Weiss,et al.  A higher-level phylogenetic classification of the Fungi. , 2007, Mycological research.

[14]  Pierre Taberlet,et al.  ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases , 2010, BMC Microbiology.

[15]  Rytas Vilgalys,et al.  Fungal Community Analysis by Large-Scale Sequencing of Environmental Samples , 2005, Applied and Environmental Microbiology.

[16]  Andy F. S. Taylor,et al.  The UNITE database for molecular identification of fungi--recent updates and future perspectives. , 2010, The New phytologist.

[17]  Rob Knight,et al.  UniFrac – An online tool for comparing microbial community diversity in a phylogenetic context , 2006, BMC Bioinformatics.