Influence of copper oxide nanoparticle form and shape on toxicity and bioaccumulation in the deposit feeder, Capitella teleta.

Few in vivo studies have been conducted to assess how nanoparticle (NP) characteristics such as particle form and shape affect their toxicity and bioaccumulation. In the present study, the deposit feeder, Capitella teleta, was used to investigate the influence of copper form (CuO NPs, micron-sized CuO particles, and aqueous Cu) and CuO NP shape (spheres, rods and platelets) on toxicity and bioaccumulation through sediment exposures of approximately 250 μg Cu/g dw sed. There were no effects of nanoparticle form or shape on mortality or growth rate during the exposure period. However, mortality increased to approximately 26.3% on average in all Cu treatments after the depuration period indicating a delayed effect of Cu exposure, despite more than 90% depuration of Cu during this period. A significant effect of nanoparticle shape was detected on body burden, the gross uptake rate constant and the depuration rate constant, suggesting preferential accumulation of rods by the worms. We recommend that additional sublethal endpoints and longer exposure durations should be examined to fully understand the environmental risks of CuO nanoparticles compared to other forms of Cu entering marine sediment systems.

[1]  M. C. Newman,et al.  Fundamentals of Ecotoxicology, Second Edition , 2002 .

[2]  D. Linton,et al.  Feeding, growth, and fecundity of Capitella sp. I in relation to sediment organic concentration , 2000 .

[3]  T. Xia,et al.  Toxic Potential of Materials at the Nanolevel , 2006, Science.

[4]  Huajian Gao,et al.  Mechanics of receptor-mediated endocytosis. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Romero,et al.  Population dynamics and production of the polychaete Capitella capitata in the littoral zone of Barcelona (Spain, NW Mediterranean) , 1997 .

[6]  Kwan Kim,et al.  A practical procedure for producing silver nanocoated fabric and its antibacterial evaluation for biomedical applications. , 2007, Chemical communications.

[7]  Marie Carrière,et al.  Size-, composition- and shape-dependent toxicological impact of metal oxide nanoparticles and carbon nanotubes toward bacteria. , 2009, Environmental science & technology.

[8]  S. Bharathiraja,et al.  Metallothionein induction and antioxidative responses in the estuarine polychaeta Capitella capitata (Capitellidae) , 2012 .

[9]  E. Valsami-Jones,et al.  Behavioural and biochemical responses of two marine invertebrates Scrobicularia plana and Hediste diversicolor to copper oxide nanoparticles. , 2011, Chemosphere.

[10]  J. Levinton,et al.  Ecology of Deposit-Feeding Animals in Marine Sediments , 1987, The Quarterly Review of Biology.

[11]  Paul Westerhoff,et al.  Nanoparticle silver released into water from commercially available sock fabrics. , 2008, Environmental science & technology.

[12]  Chengfang Pang,et al.  Bioaccumulation, toxicokinetics, and effects of copper from sediment spiked with aqueous Cu, nano‐CuO, or micro‐CuO in the deposit‐feeding snail, Potamopyrgus antipodarum , 2013, Environmental toxicology and chemistry.

[13]  J. Grassle,et al.  Opportunistic life histories and genetic systems in marine benthic polychaetes , 1974 .

[14]  Kyunghee Choi,et al.  Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. , 2010, Toxicology in vitro : an international journal published in association with BIBRA.

[15]  Stephanie E. A. Gratton,et al.  The effect of particle design on cellular internalization pathways , 2008, Proceedings of the National Academy of Sciences.

[16]  I. Zuhorn,et al.  Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis. , 2004, The Biochemical journal.

[17]  Arezou A Ghazani,et al.  Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. , 2006, Nano letters.

[18]  Gary M. Rand,et al.  Fundamentals of Aquatic Toxicology , 1985 .

[19]  Chengfang Pang,et al.  Effects of sediment-associated copper to the deposit-feeding snail, Potamopyrgus antipodarum: a comparison of Cu added in aqueous form or as nano- and micro-CuO particles. , 2012, Aquatic toxicology.

[20]  Jing Luo,et al.  Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms , 2008, Environmental toxicology and chemistry.

[21]  Mark Turmaine,et al.  Cellular internalization of silver nanoparticles in gut epithelia of the estuarine polychaete Nereis diversicolor. , 2011, Environmental science & technology.

[22]  V. Forbes,et al.  Toxic effects and bioaccumulation of nano-, micron- and ionic-Ag in the polychaete, Nereis diversicolor. , 2011, Aquatic toxicology.

[23]  B. Rygg.  Effect of sediment copper on benthic fauna , 1985 .

[24]  E. Valsami-Jones,et al.  A mesocosm study of fate and effects of CuO nanoparticles on endobenthic species (Scrobicularia plana, Hediste diversicolor). , 2013, Environmental science & technology.

[25]  Deborah Berhanu,et al.  Comparative study using spheres, rods and spindle-shaped nanoplatelets on dispersion stability, dissolution and toxicity of CuO nanomaterials , 2014, Nanotoxicology.

[26]  Valery E. Forbes,et al.  Effects, Uptake, and Depuration Kinetics of Silver Oxide and Copper Oxide Nanoparticles in a Marine Deposit Feeder, Macoma balthica , 2013 .

[27]  G. Lopez,et al.  THE ALLOMETRY OF DEPOSIT FEEDING IN CAPITELLA SPECIES I (POLYCHAETA:CAPITELLIDAE): THE ROLE OF TEMPERATURE AND PELLET WEIGHT IN THE CONTROL OF EGESTION , 1987 .

[28]  Warren C W Chan,et al.  Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. , 2007, Nano letters.

[29]  Maria João Bebianno,et al.  Accumulation and toxicity of copper oxide nanoparticles in the digestive gland of Mytilus galloprovincialis. , 2012, Aquatic toxicology.

[30]  J. Grassle,et al.  Capitella teleta , a new species designation for the opportunistic and experimental Capitella sp. I, with a review of the literature for confirmed records , 2009 .

[31]  J. Song,et al.  Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli , 2007, Applied and Environmental Microbiology.

[32]  Simon Labrecque,et al.  Microglial response to gold nanoparticles. , 2010, ACS nano.

[33]  R. Scholz,et al.  Modeled environmental concentrations of engineered nanomaterials (TiO(2), ZnO, Ag, CNT, Fullerenes) for different regions. , 2009, Environmental science & technology.

[34]  J. Stafford,et al.  Evaluating the toxicity of hydroxyapatite nanoparticles in catfish cells and zebrafish embryos. , 2013, Small.

[35]  V. Forbes,et al.  Bioaccumulation and effects of different‐shaped copper oxide nanoparticles in the deposit‐feeding snail Potamopyrgus antipodarum , 2014, Environmental toxicology and chemistry.

[36]  V. Forbes,et al.  Life history and population dynamics of the opportunistic polychaete Capitella sp. I in relation to sediment organic matter , 2008 .