Morphological and physiological changes of interstitial cells of Cajal after small bowel transplantation in rats

Intestinal dysmotility has been reported to be associated with a decreased number of interstitial cells of Cajal (ICCs). However, the chronological changes in ICCs after small bowel transplantation (SBT) have not yet been elucidated. In this study, we aimed to evaluate the chronological change of ICCs after SBT. Orthotopic syngeneic SBT was performed in rats. Graft specimens were obtained at postreperfusion, and on 1, 3, 7, 14, and 30 postoperative day (POD). Thereafter, immunohistochemical staining was performed and the spontaneous contractions measured. During the initial period after SBT, the temporal impairment of ICCs was found. In an immunohistochemical study, c‐Kit‐positive cells appeared to decrease on POD 0, 1, and 3. Thereafter, the number of cells increased gradually up to POD 7. In contrast, the recovery of the spontaneous contractile amplitude took more time. The frequency of the electrical signal was preserved at almost exactly the same levels throughout this experimental period. Although the network of ICCs was found to be temporarily impaired after SBT in an immunohistochemical examination, this change was reversible. Moreover, the recovery of the function of the intestinal motility associated with ICCs was delayed after the early postoperative period.

[1]  T. Nakaki,et al.  Interstitial cells of Cajal in dysmotility in intestinal ischemia and reperfusion injury in rats. , 2006, The Journal of surgical research.

[2]  K. Won,et al.  Motility disorder in experimentally obstructed intestine: relationship between muscularis inflammation and disruption of the ICC network , 2006, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[3]  A. Nakao,et al.  Inherent pacemaker function of duodenal GIST. , 2006, European journal of cancer.

[4]  A. Rühl.  Glial cells in the gut , 2005, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[5]  M. Sarr,et al.  Inhibition by nitric oxide and nonadrenergic, noncholinergic (NANC) nerves is preserved in a canine model of extrinsic denervation: implications for small bowel transplantation. , 2005, Surgery.

[6]  J. Huizinga,et al.  Changes in interstitial cells of Cajal at the deep muscular plexus are associated with loss of distention-induced burst-type muscle activity in mice infected by Trichinella spiralis. , 2005, The American journal of pathology.

[7]  S. Suita,et al.  An abnormal distribution of C-kit positive cells in the normoganglionic segment can predict a poor clinical outcome in patients with Hirschsprung's disease. , 2005, European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie.

[8]  Y. Nakajima,et al.  Absence of peristalsis in the ileum of W/W(V) mutant mice that are selectively deficient in myenteric interstitial cells of Cajal. , 2005, Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi.

[9]  E. Ekblad,et al.  Structural and Neuronal Changes in Rat Ileum After Ischemia with Reperfusion , 2004, Digestive Diseases and Sciences.

[10]  J. Conklin,et al.  Intestinal Motility During Hypoxia and Reoxygenation in Vitro , 1997, Digestive Diseases and Sciences.

[11]  P. Puri,et al.  Distribution of heme oxygenase-2 in nerves and interstitial cells of Cajal in the normal pylorus and in infantile hypertrophic pyloric stenosis. , 2003, Archives of pathology & laboratory medicine.

[12]  S. Ward,et al.  Interstitial cells: involvement in rhythmicity and neural control of gut smooth muscle , 2003, The Journal of physiology.

[13]  R. Tomita,et al.  Physiologic Studies on Nitric Oxide in Rat Small Bowel Isografts , 2003, World Journal of Surgery.

[14]  P. Hubka,et al.  The pacemaker activity of interstitial cells of Cajal and gastric electrical activity. , 2003, Physiological research.

[15]  E. Fehér,et al.  Nitric oxide synthase immunoreactivity of interstitial cells of Cajal in experimental colitis , 2002, Inflammation Research.

[16]  M. Impicciatore,et al.  Alterations of intestinal motor responsiveness in a model of mild mesenteric ischemia/reperfusion in rats. , 2002, Life sciences.

[17]  M. Vannucchi,et al.  Alterations of neurokinin receptors and interstitial cells of Cajal during and after jejunal inflammation induced by Nippostrongylus brasiliensis in the rat , 2002, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[18]  S. Ward,et al.  Deficiency of interstitial cells of Cajal in the small intestine of patients with Crohn's disease , 2002, American Journal of Gastroenterology.

[19]  J. Huizinga,et al.  Interstitial cells of cajal and inflammation-induced motor dysfunction in the mouse small intestine. , 2000, Gastroenterology.

[20]  D. Tweardy,et al.  Molecular and functional contractile sequelae of rat intestinal ischemia/reperfusion injury. , 1999, Transplantation.

[21]  S. Suita,et al.  Abnormalities of enteric neurons, intestinal pacemaker cells, and smooth muscle in human intestinal atresia. , 1999, Journal of pediatric surgery.

[22]  S Torihashi,et al.  Blockade of kit signaling induces transdifferentiation of interstitial cells of cajal to a smooth muscle phenotype. , 1999, Gastroenterology.

[23]  S. Suita,et al.  Chronological change of distribution in nitric oxide and peptidergic neurons after rat small intestinal transplantation. , 1999, Journal of pediatric surgery.

[24]  K. Chandross.  Nerve injury and inflammatory cytokines modulate gap junctions in the peripheral nervous system , 1998, Glia.

[25]  C. Di Lorenzo,et al.  Intestinal motility after small bowel transplantation. , 1998, Transplantation proceedings.

[26]  J. Huizinga.  IV. Pathophysiology of GI motility related to interstitial cells of Cajal. , 1998, American journal of physiology. Gastrointestinal and liver physiology.

[27]  D. Lloyd,et al.  Abnormal colonic interstitial cells of Cajal in children with anorectal malformations. , 1998, Journal of pediatric surgery.

[28]  J. Huizinga.  Neural injury, repair, and adaptation in the GI tract. IV. Pathophysiology of GI motility related to interstitial cells of Cajal. , 1998, The American journal of physiology.

[29]  S. Ward,et al.  Development of c-Kit-positive cells and the onset of electrical rhythmicity in murine small intestine. , 1997, Gastroenterology.

[30]  J. Rumessen.  Ultrastructure of interstitial cells of Cajal at the colonic submuscular border in patients with ulcerative colitis. , 1996, Gastroenterology.

[31]  J. Rumessen,et al.  Interstitial cells of Cajal in human colon and in Hirschsprung's disease. , 1996, Gastroenterology.

[32]  J. Vanderwinden,et al.  Study of the interstitial cells of Cajal in infantile hypertrophic pyloric stenosis. , 1996, Gastroenterology.

[33]  G. Gabella,et al.  Origin of the c-kit-positive interstitial cells in the avian bowel. , 1996, Development.

[34]  S. Suita,et al.  Immunohistochemical demonstration of enteric nervous distribution after syngeneic small bowel transplantation in rats. , 1995, Surgery.

[35]  Y. Shimahara,et al.  EXTRINSIC SYMPATHETIC REINNERVATION AFTER INTESTINAL TRANSPLANTATION IN RATS , 1995, Transplantation.

[36]  W. Gerthoffer,et al.  Interstitial cells in deep muscular plexus of canine small intestine may be specialized smooth muscle cells. , 1993, The American journal of physiology.