Coconut Water Based Extender Effects on Motility,Viability, and DNA Integrity of Chilled Kintamani Dog Semen

This research was conducted to investigate coconut water based extenders as an alternative extender for chilled Kintamani dog semen. Semen were collected from five dogs using manual stimulation and evaluated microscopically. Sperm were stored at 5 0 C and divided into two aliquots which were extended with either coconut water based extender and sodium citrate extender in the ratio 1:2, 1:3 and 1:4. Sperm motility, viability and DNA integrity immediately and periodically (0,3 and 6 hour) were evaluated. Result showed that the effect of diluent on sperm motility and integrity of the Kintamani dog exerted a highly significant (p 0.05). These results indicate that coconut water extender give good results in maintaining the motility, viability and DNA integrityof Kintamani dog sperm. It is concluded that coconut water suitable for extender of Kintamani dog.

[1]  H. Rodríguez-Martínez,et al.  Sperm morphology and chromatin integrity in Swedish warmblood stallions and their relationship to pregnancy rates , 2008, Acta veterinaria Scandinavica.

[2]  J. Gosálvez,et al.  A new method to analyze boar sperm DNA fragmentation under bright-field or fluorescence microscopy. , 2006, Theriogenology.

[3]  N. Pedersen,et al.  The Kintamani dog: genetic profile of an emerging breed from Bali, Indonesia. , 2005, The Journal of heredity.

[4]  B. Essén-Gustavsson,et al.  Influence of glucose and fructose in the extender during long-term storage of chilled canine semen. , 2004, Theriogenology.

[5]  A. Giwercman,et al.  Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays. , 2004, Human reproduction.

[6]  E. Kawakami,et al.  Artificial insemination with canine semen stored at a low temperature. , 2003, The Journal of veterinary medical science.

[7]  A. R. Silva,et al.  Cryopreservation of canine semen using a coconut water extender with egg yolk and three different glycerol concentrations. , 2003, Theriogenology.

[8]  David Heath,et al.  An Introduction To Experimental Design And Statistics For Biology , 1995 .

[9]  S. Johnston.  Performing a complete canine semen evaluation in a small animal hospital. , 1991, The Veterinary clinics of North America. Small animal practice.

[10]  C. Linde-Forsberg.  Achieving canine pregnancy by using frozen or chilled extended semen. , 1991, The Veterinary clinics of North America. Small animal practice.

[11]  J. Mitchell,et al.  A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. , 1984, Fertility and sterility.

[12]  A. Bamunuarachchi,et al.  Manufacture of carbonated tender coconut water and development of a process for the utilization of coconut flesh. , 2009 .