Effects of steady electric fields on human retinal pigment epithelial cell orientation and migration in culture

Abstract Low‐level, steady electric fields of 6–10 volts/cm stimulated directional orientation and translocation of cultured human retinal pigment epithelial cells. The orientative movements (galvanotropism) consisted of somatic elongation of the cells into spindle shapes, followed by pivotal alignment orthogonal to the field. The anodal edges of the cells underwent retraction of their plasmalemmal extensions, while the cathode edges and the longitudinal ends developed lamellipodia and ruffled membranes. These tropic movements were followed by a translocational movement (galvanotaxis) of the cells towards the cathode. Staining of these migrating cells for actin showed the accumulation of stress fibers at the leading (cathodal) edge, as well as at the longitudinal ends of the elongated somata. These results suggest that endogenous, biologically‐generated electric fields (eg., injury currents) may play a role in the guidance and migration of retinal pigment epithelial cells after retinal injury.

[1]  L. Jaffe,et al.  Strong electrical currents leave the primitive streak of chick embryos. , 1979, Science.

[2]  L. Jaffe,et al.  Neurites grow faster towards the cathode than the anode in a steady field. , 1979, The Journal of experimental zoology.

[3]  J. Anderson,et al.  GALVANOTAXIS OF SLIME MOLD , 1951, The Journal of general physiology.

[4]  P. W. Luther,et al.  Changes in cell shape and actin distribution induced by constant electric fields , 1983, Nature.

[5]  C. McCaig,et al.  The direction of growth of differentiating neurones and myoblasts from frog embryos in an applied electric field. , 1981, The Journal of physiology.

[6]  M. Poo,et al.  Orientation of neurite growth by extracellular electric fields , 1982, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[7]  J. D. Feldman,et al.  Directional protrusive pseudopodial activity and motility in macrophages induced by extracellular electric fields. , 1982, Cell motility.

[8]  K. R. Robinson,et al.  Xenopus neural crest cell migration in an applied electrical field , 1983, The Journal of cell biology.

[9]  R. Nuccitelli,et al.  Embryonic cell motility can be guided by physiological electric fields. , 1983, Experimental cell research.

[10]  S. Hui,et al.  Response of C3H/10T1/2 fibroblasts to an external steady electric field stimulation. Reorientation, shape change, ConA receptor and intramembranous particle distribution and cytoskeleton reorganization. , 1984, Experimental cell research.

[11]  K R Robinson,et al.  The responses of cells to electrical fields: a review , 1985, The Journal of cell biology.

[12]  B. Rollins,et al.  Monocyte chemotactic protein gene expression by cytokine-treated human retinal pigment epithelial cells. , 1991, Laboratory investigation; a journal of technical methods and pathology.

[13]  C. McCaig,et al.  The ontogeny of the transepidermal potential difference in frog embryos. , 1982, Developmental biology.

[14]  E. Lazarides,et al.  Actin antibody: the specific visualization of actin filaments in non-muscle cells. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[15]  H. Soong,et al.  Effects of electric fields on cytoskeleton of corneal stromal fibroblasts. , 1990, Current eye research.

[16]  H. Soong,et al.  Movements of cultured corneal epithelial cells and stromal fibroblasts in electric fields. , 1990, Investigative ophthalmology & visual science.

[17]  Richard Nuccitelli,et al.  AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS , 1974, The Journal of cell biology.

[18]  R. Nuccitelli.  Physiological Electric Fields can Influence Cell Motility, Growth, and Polarity , 1988 .

[19]  J. Aubin,et al.  Osteoclasts and osteoblasts migrate in opposite directions in response to a constant electrical field , 1986, Journal of cellular physiology.

[20]  R. Nuccitelli,et al.  Embryonic fibroblast motility and orientation can be influenced by physiological electric fields , 1984, The Journal of cell biology.

[21]  K. R. Robinson,et al.  Self‐generated electrical currents through Xenopus neurulae. , 1984, The Journal of physiology.

[22]  M. S. Cooper,et al.  Motility of cultured fish epidermal cells in the presence and absence of direct current electric fields , 1986, The Journal of cell biology.

[23]  K. Weber,et al.  Three-dimensional organization of microfilaments and microtubules in the cytoskeleton. Immunoperoxidase labelling and stereo-electron microscopy of detergent-extracted cells. , 1979, Experimental cell research.