Protective effects of hyperoside (quercetin-3-o-galactoside) to PC12 cells against cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide.

Reactive oxygen species are believed to play a very significant role in the pathogenesis of several diseases including Alzheimer's disease and Parkinson's disease. It is reported that the crucial balance between reactive oxygen species generation and antioxidant defense is regarded as a force in a wide variety of chronic diseases. In this paper, PC12 cells were used to study the antioxidative effect of hyperoside. The results indicated that hyperoside could effectively protect PC12 cells against cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide at 160 microg/ml and 100 microg/ml, respectively. The study also showed that hyperoside was no harmful within the tested concentration range and could easily enter into the PC12 cells. With the increasing concentration of hyperoside, cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide was significantly attenuated and the corresponding extracellular lactate dehydrogenase levels decreased concurrently by pretreatment with hyperoside. The results were proved by flow cytometric detection of apoptotic cells. All the above results showed hyperoside could efficiently prevent the PC12 cells from shrinking and turning against apoptosis induced by hydrogen peroxide and tert-butyl hydroperoxide.

[1]  C E Koop,et al.  Reducing health care costs by reducing the need and demand for medical services. The Health Project Consortium. , 1993, The New England journal of medicine.

[2]  E. Ernst,et al.  St John's wort: Prozac from the plant kingdom. , 2001, Trends in pharmacological sciences.

[3]  Yu Huang,et al.  Characterization of antioxidants present in hawthorn fruits. , 2001, The Journal of nutritional biochemistry.

[4]  M. Brandi,et al.  Flavonoids: biochemical effects and therapeutic applications. , 1992, Bone and mineral.

[5]  L. Luo,et al.  Inhibitory effects of flavonoids from Hypericum perforatum on nitric oxide synthase. , 2004, Journal of ethnopharmacology.

[6]  F. Liu,et al.  Antioxidative and free radical scavenging activities of selected medicinal herbs. , 2000, Life sciences.

[7]  Y. Sugita,et al.  Kinetic studies on the removal of extracellular hydrogen peroxide by cultured fibroblasts. , 1994, The Journal of biological chemistry.

[8]  P. Houghton,et al.  Antioxidant activity of methanolic extract of Hypericum triquetrifolium Turra aerial part. , 2002, Fitoterapia.

[9]  M. Couladis,et al.  Antioxidant and cytotoxic activities of Hypericum sp. on brine shrimps and human cancer cell lines , 2002, Phytotherapy research : PTR.

[10]  L G Korkina,et al.  Antioxidant and chelating properties of flavonoids. , 1997, Advances in pharmacology.

[11]  R. S. Sohal.  Oxidative stress hypothesis of aging. , 2002, Free radical biology & medicine.

[12]  G. Lescoat,et al.  Role of flavonoids and iron chelation in antioxidant action. , 1994, Methods in enzymology.

[13]  N. Takeyama,et al.  DNA damage induced by tumour necrosis factor-alpha in L929 cells is mediated by mitochondrial oxygen radical formation. , 1995, Immunology.

[14]  G. Lescoat,et al.  Antioxidant and iron-chelating activities of the flavonoids catechin, quercetin and diosmetin on iron-loaded rat hepatocyte cultures. , 1993, Biochemical pharmacology.

[15]  Chung-Soo Lee,et al.  Protective effect of serotonin on 6-hydroxydopamine- and dopamine-induced oxidative damage of brain mitochondria and synaptosomes and PC12 cells , 2002, Neurochemistry International.

[16]  T. Finkel.  Oxygen radicals and signaling. , 1998, Current opinion in cell biology.

[17]  C. Cotman,et al.  Exposure to hydrogen peroxide induces cell death via apoptosis in cultured rat cortical neurons , 1994, Neuroreport.

[18]  D. Wei,et al.  The protective effect of ascorbic acid derivative on PC12 cells: involvement of its ROS scavenging ability. , 2003, Life sciences.

[19]  A. Bendich,et al.  Free radical tissue damage: protective role of antioxidant nutrients 1 , 1987, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[20]  D. Wei,et al.  Ascorbic acid-2-o-phosphate-6-o-palmitate protecting the human umbilical cord vein endothelial cells against hydrogen peroxide and tert-butyl hydroperoxide induced cytotoxicity. , 2004, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[21]  S. Ryu,et al.  Phospholipase D2 Activity Suppresses Hydrogen Peroxide‐Induced Apoptosis in PC12 Cells , 2000, Journal of neurochemistry.

[22]  J. Pommery,et al.  Antioxidant properties of hydroxy-flavones. , 1996, Free radical biology & medicine.

[23]  M. Beal.  Oxidatively modified proteins in aging and disease. , 2002, Free radical biology & medicine.

[24]  A. I. Dorozhko,et al.  Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation. , 1989, Biochemical pharmacology.

[25]  D. Lobner.  Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis? , 2000, Journal of Neuroscience Methods.

[26]  H. Kamata,et al.  Redox regulation of cellular signalling. , 1999, Cellular signalling.

[27]  S. Orrenius,et al.  Triggering and modulation of apoptosis by oxidative stress. , 2000, Free radical biology & medicine.

[28]  D. Allan Butterfield,et al.  Brain protein oxidation in age-related neurodegenerative disorders that are associated with aggregated proteins , 2001, Mechanisms of Ageing and Development.

[29]  N. Sugihara,et al.  Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with alpha-linolenic acid. , 1999, Free radical biology & medicine.

[30]  V. Mišík,et al.  Redox intermediates of flavonoids and caffeic acid esters from propolis: an EPR spectroscopy and cyclic voltammetry study. , 1995, Free radical biology & medicine.

[31]  U. Hafner,et al.  Hyperforin represents the neurotransmitter reuptake inhibiting constituent of hypericum extract. , 1998, Pharmacopsychiatry.

[32]  D. Wei,et al.  Neuroprotective effects of Hypericum perforatum on trauma induced by hydrogen peroxide in PC12 cells. , 2004, The American journal of Chinese medicine.

[33]  S. Martín-Aragón,et al.  Antioxidant properties and protective effects of a standardized extract of Hypericum perforatum on hydrogen peroxide-induced oxidative damage in PC12 cells. , 2004, Life sciences.

[34]  B. Bauer,et al.  Ephedra-containing dietary supplements in the US versus ephedra as a Chinese medicine. , 2004, The American journal of Chinese medicine.

[35]  B Havsteen,et al.  Flavonoids, a class of natural products of high pharmacological potency. , 1983, Biochemical pharmacology.

[36]  A. Navarrete,et al.  Amoebicidal and giardicidal compounds from the leaves of Zanthoxylum liebmannianun. , 2001, Fitoterapia.

[37]  B Chance,et al.  Hydroperoxide metabolism in mammalian organs. , 1979, Physiological reviews.

[38]  M. Torsoni,et al.  t-BOOH-induced oxidative damage in sickle red blood cells and the role of flavonoids. , 2003, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[39]  A. Attia,et al.  Hypericum perforatum extract demonstrates antioxidant properties against elevated rat brain oxidative status induced by amnestic dose of scopolamine , 2003, Pharmacology Biochemistry and Behavior.

[40]  A. Boveris.  Determination of the production of superoxide radicals and hydrogen peroxide in mitochondria. , 1984, Methods in enzymology.

[41]  D. Wei,et al.  Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. , 2004, Journal of agricultural and food chemistry.

[42]  P. Hollman,et al.  Absorption, metabolism and health effects of dietary flavonoids in man. , 1997, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.