Temperature dependence of immunoreactions using shear horizontal surface acoustic wave immunosensors

In this paper, the temperature dependence of immunoreactions, which are antibody–antigen reactions, on a shear horizontal surface acoustic wave (SH-SAW) immunosensor is described. The immunosensor is based on a reflection-type delay line on a 36° Y-cut 90° X-propagation quartz substrate, where the delay line is composed of a floating electrode unidirectional transducer (FEUDT), a grating reflector, and a sensing area between them. In order to evaluate the temperature dependence of immunoreactions, human serum albumin (HSA) antigen–antibody reactions are investigated. The SH-SAW immunosensor chip is placed in a thermostatic chamber and the changes in the SH-SAW velocity resulting from the immunoreactions are measured at different temperatures. As a result, it is observed that the HSA immunoreactions are influenced by the ambient temperature and that higher temperatures provide more active reactions. In order to analyze the immunoreactions, an analytical approach using an exponential fitting method for changes in SH-SAW velocity is employed.

[1]  Muhammad Sajid,et al.  Designs, formats and applications of lateral flow assay: A literature review , 2015 .

[2]  S. Shiokawa,et al.  Novel atomization method based on SAW streaming , 2003, IEEE Symposium on Ultrasonics, 2003.

[3]  Y. Nagasaki,et al.  High-performance surface acoustic wave immunosensing system on a PEG/aptamer hybridized surface. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[4]  D. J. Finney,et al.  Guidelines for immunoassay data processing. , 1985, Clinical chemistry.

[5]  Rayleigh SAW assisted SH-SAW immunosensor on X-cut 148-Y LiTaO3 , 2016 .

[6]  Kohji Mitsubayashi,et al.  Repetitive Immunoassay with a Surface Acoustic Wave Device and a Highly Stable Protein Monolayer for On-Site Monitoring of Airborne Dust Mite Allergens. , 2015, Analytical chemistry.

[7]  Shangjr Gwo,et al.  Quantitative surface acoustic wave detection based on colloidal gold nanoparticles and their bioconjugates. , 2008, Analytical chemistry.

[8]  Larry J Kricka,et al.  Improving healthcare accessibility through point-of-care technologies. , 2007, Clinical chemistry.

[9]  Jun Kondoh,et al.  Study of Surface Acoustic Wave Streaming Phenomenon Based on Temperature Measurement and Observation of Streaming in Liquids , 2007 .

[10]  Jun Kondoh,et al.  Development of temperature-control system for liquid droplet using surface Acoustic wave devices , 2009 .

[11]  D. Steinhilber,et al.  Molecular biology in medicinal chemistry , 2004 .

[12]  Nam-Trung Nguyen,et al.  Surface Acoustic Wave Driven Microfluidics – A Review , 2010 .

[13]  Raphael C. Wong,et al.  Lateral flow immunoassay , 2009 .

[14]  J Witz,et al.  Thermodynamic analysis of antigen-antibody binding using biosensor measurements at different temperatures. , 1997, Analytical biochemistry.

[15]  H. Yatsuda,et al.  3F-3 Liquid Sensor Using SAW and SH-SAW on Quartz , 2006, 2006 IEEE Ultrasonics Symposium.

[16]  S. Piletsky,et al.  In vitro diagnostics in diabetes: meeting the challenge. , 1999, Clinical chemistry.

[17]  Bruce D. Hammock,et al.  Competitive immunochromatographic assay for the detection of the organophosphorus pesticide EPN , 2013 .

[18]  Vincent Aimez,et al.  Integrated active mixing and biosensing using surface acoustic waves (SAW) and surface plasmon resonance (SPR) on a common substrate. , 2010, Lab on a chip.

[19]  Qing-Ming Wang,et al.  Monitoring the adhesion process of tendon stem cells using shear-horizontal surface acoustic wave sensors , 2015, 2015 Joint Conference of the IEEE International Frequency Control Symposium & the European Frequency and Time Forum.

[20]  H. Yatsuda,et al.  Liquid-Phase Shear Horizontal Surface Acoustic Wave Immunosensor , 2010 .

[21]  D. Chan,et al.  Immunosensors--principles and applications to clinical chemistry. , 2001, Clinica chimica acta; international journal of clinical chemistry.

[22]  K. Yamanouchi,et al.  New low-loss SAW filter using internal floating electrode reflection types of single-phase unidirectional transducer , 1984 .

[23]  Peter B. Luppa,et al.  Point-of-care testing (POCT): Current techniques and future perspectives , 2011, TrAC Trends in Analytical Chemistry.

[24]  C P Price,et al.  Point of Care Testing , 1999, BMJ : British Medical Journal.

[25]  B. Hammock,et al.  Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos. , 2011, Analytica chimica acta.

[26]  F. Sarry,et al.  Rayleigh surface acoustic wave as an efficient heating system for biological reactions: investigation of microdroplet temperature uniformity , 2015, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control.

[27]  T. Moriizumi,et al.  Experimental Study on Liquid Streaming by SAW , 1989 .

[28]  Bastian E. Rapp,et al.  Surface acoustic wave biosensors: a review , 2008, Analytical and bioanalytical chemistry.