Alternative splicing induced by insertion of retrotransposons into the maize waxy gene.

The molecular basis for the low level expression of three alleles of the maize waxy (Wx) gene has been described. Each allele contains a retrotransposon in intron sequences. These insertions represent previously undescribed elements, and their association with three wx alleles indicates that retrotransposon elements are important agents of spontaneous mutation in maize. For each allele, element sequences are spliced from pre-mRNA with the surrounding intron even though the insertions increase intron length by approximately 40- to 60-fold. In addition, despite differences in element sequences, insertion sites, and relative orientations, each element disrupts long-range splice site recognition leading to novel Wx transcripts where exons both upstream and downstream of the insertion site are skipped. The expression of wx alleles with large insertions in introns provides support for studies that indicate that the primary cis requirement for maize introns is the splice donor and acceptor sites.

[1]  S. Wessler.  The maize transposable Ds1 element is alternatively spliced from exon sequences , 1991, Molecular and cellular biology.

[2]  M. Freeling,et al.  A low copy number, copia‐like transposon in maize. , 1985, The EMBO journal.

[3]  M. Scott,et al.  The molecular organization of the Antennapedia locus of drosophila , 1983, Cell.

[4]  H. Saedler,et al.  Molecular analysis of the En/Spm transposable element system of Zea mays , 1986, The EMBO journal.

[5]  V. Corces,et al.  Interactions of retrotransposons with the host genome: the case of the gypsy element of Drosophila. , 1991, Trends in genetics : TIG.

[6]  L. McConlogue,et al.  Structure-independent DNA amplification by PCR using 7-deaza-2'-deoxyguanosine. , 1988, Nucleic acids research.

[7]  D. Finnegan,et al.  Transposable elements in Drosophila melanogaster. , 1982, Oxford surveys on eukaryotic genes.

[8]  D. Voytas,et al.  A copia-like transposable element family in Arabidopsis thaliana , 1988, Nature.

[9]  H. Saedler,et al.  Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays , 1985, The EMBO journal.

[10]  Thomas W. Glover,et al.  A de novo Alu insertion results in neurofibromatosis type 1 , 1991, Nature.

[11]  M. Simsek,et al.  Nucleotide sequence of wheat germ cytoplasmic initiator methionine transfer ribonucleic acid. , 1982, Nucleic acids research.

[12]  J. Schiefelbein,et al.  Deletions in a dspm insert in a maize bronze-1 allele alter RNA processing and gene expression. , 1989, Genetics.

[13]  Finnegan Dj,et al.  Transposable elements in Drosophila melanogaster. , 1986 .

[14]  S. Wessler,et al.  Isolation of the transposable maize controlling elements Ac and Ds , 1983, Cell.

[15]  J. Boeke,et al.  Transcription and reverse transcription of retrotransposons. , 1989, Annual review of microbiology.

[16]  S. Wessler,et al.  Molecular basis of mutations at the waxy locus of maize: correlation with the fine structure genetic map. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[17]  S. Wessler,et al.  Molecular identification and isolation of the Waxy locus in maize , 1983, Cell.

[18]  J. Graves,et al.  Mobile element insertions causing mutations in the Drosophila suppressor of sable locus occur in DNase I hypersensitive subregions of 5'-transcribed nontranslated sequences. , 1990, Genetics.

[19]  P. Bingham,et al.  Regulation of white locus expression: The structure of mutant alleles at the white locus of Drosophila melanogaster , 1982, Cell.

[20]  S. Wessler,et al.  Excision of Ds produces waxy proteins with a range of enzymatic activities. , 1986, The EMBO journal.

[21]  J. Vieira,et al.  Production of single-stranded plasmid DNA. , 1987, Methods in enzymology.

[22]  O. Nelson,et al.  The enzymatic deficiency in the waxy mutant of maize. , 1962, Biochemical and biophysical research communications.

[23]  J. Schiefelbein,et al.  RNA splicing permits expression of a maize gene with a defective Suppressor-mutator transposable element insertion in an exon. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[24]  G. Goodall,et al.  Different effects of intron nucleotide composition and secondary structure on pre‐mRNA splicing in monocot and dicot plants. , 1991, The EMBO journal.

[25]  S. Wessler,et al.  The splicing of maize transposable elements from pre-mRNA--a minireview. , 1989, Gene.

[26]  Stuart H. Orkin,et al.  Abnormal RNA splicing causes one form of α thalassemia , 1982, Cell.

[27]  R. Flavell,et al.  A family of retrotransposons and associated genomic variation in wheat. , 1991, Genomics.

[28]  D. E. Ruffner,et al.  Splicing mutation in human hereditary analbuminemia. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[29]  S. Schuster,et al.  Identification of an altered splice site in Ashkenazi Tay-Sachs disease , 1988, Nature.

[30]  V. Corces,et al.  Drosophila transposable elements: mechanisms of mutagenesis and interactions with the host genome. , 1991, Advances in genetics.

[31]  G. Rubin,et al.  Effects of transposable element insertions on RNA encoded by the white gene of Drosophila , 1984, Cell.

[32]  M. Frohman,et al.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[33]  S. Wessler,et al.  The Effects of Plant Transposable Element Insertion on Transcription Initiation and RNA Processing , 1990 .

[34]  D. Smyth,et al.  Plant retrotransposon from Lilium henryi is related to Ty3 of yeast and the gypsy group of Drosophila. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[35]  O. Nelson.  The WAXY Locus in Maize. II. the Location of the Controlling Element Alleles. , 1968, Genetics.

[36]  D. Voytas,et al.  The structure, distribution and evolution of the Ta1 retrotransposable element family of Arabidopsis thaliana. , 1990, Genetics.

[37]  Tom Maniatis,et al.  A single-base change at a splice site in a β0-thalassemic gene causes abnormal RNA splicing , 1982, Cell.

[38]  P. Schnable,et al.  The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene. , 1990, The EMBO journal.