In-situ lipopeptide biosurfactant production by Bacillus strains correlates with improved oil recovery in two oil wells approaching their economic limit of production

Abstract Significant amounts of entrapped oil could be potentially recovered from water-flooded reservoirs nearing their economic limit of production via biosurfactant-enhanced oil recovery. However, evidence for the persistence and metabolic activity of injected biosurfactant-producing bacterial species activity is lacking. We injected a glucose-nitrate-mineral nutrient mixture and two lipopeptide biosurfactant-producing Bacillus strains into two wells to correlate in-situ metabolism with oil recovery. Two wells producing from the same Viola formation were each inoculated with about 60 m 3 of tank battery brine with a nutrient mixture containing glucose, sodium nitrate and trace metals and Bacillus licheniformis RS-1 and Bacillus subtilis subsp. subtilis spizizenii NRRL B-23049. Analysis of production water indicated in-situ growth of the injected strains and other heterotrophic fermenting bacteria, metabolism of the nutrients, and biosurfactant production. Both wells had a peak lipopeptide biosurfactant concentration of 20 and 28 mg/L, respectively, which is twice the minimum concentration required to mobilize entrapped oil from sandstone cores. Metabolic products of glucose fermentation in both wells were acetate, 2,3-butanediol, ethanol, formate, lactate, and succinate and cells and these products accounted for 107.6% of the glucose used. The increase in biosurfactant, acids, alcohols and carbon dioxide during the first 5 days after commencement of production corresponded directly with an increase in oil recovery. Wellhead measurements of total produced fluid, the water-to-oil ratio and the percentage of oil showed that about 52.5 m 3 of additional oil (net cumulative increase) occurred during the first 60 days of sampling. These results showed the feasibility of stimulating in-situ biosurfactant production and its potential to improved oil production from mature oil reservoirs.

[1]  S. Cameotra,et al.  Biosurfactant production by a thermophilic Bacillus subtilis strain , 1997, Journal of Industrial Microbiology and Biotechnology.

[2]  A. Grigor’yan,et al.  Production of Oil-Releasing Compounds by Microorganisms from the Daqing Oil Field, China , 2003, Microbiology.

[3]  J. A. Roels,et al.  Energetics and Kinetics in Biotechnology , 1983 .

[4]  Vikas Pruthi,et al.  Production of a biosurfactant exhibiting excellent emulsification and surface active properties bySerratia marcescens , 1997 .

[5]  Sumaeth Chavadej,et al.  Isolation and comparison of biosurfactants produced by Bacillus subtilis PT2 and Pseudomonas aeruginosa SP4 for microbial surfactant-enhanced oil recovery , 2008 .

[6]  Roy M. Knapp,et al.  Microbially Enhanced Oil Recovery: Past, Present, and Future , 2005 .

[7]  U. Zoller,et al.  Handbook of detergents , 1999 .

[8]  D. Sabatini,et al.  Integrated design of surfactant enhanced DNAPL remediation: efficient supersolubilization and gradient systems , 2000 .

[9]  D. Sabatini,et al.  Surfactant Selection Criteria for Enhanced Subsurface Remediation , 1999 .

[10]  R. M. Knapp,et al.  In Situ Biosurfactant Production by Bacillus Strains Injected into a Limestone Petroleum Reservoir , 2006, Applied and Environmental Microbiology.

[11]  N. Youssef,et al.  Comparison of methods to detect biosurfactant production by diverse microorganisms. , 2004, Journal of microbiological methods.

[12]  M. McInerney,et al.  Biosurfactant-producing Bacillus are present in produced brines from Oklahoma oil reservoirs with a wide range of salinities , 2011, Applied Microbiology and Biotechnology.

[13]  Jeffrey H. Harwell,et al.  Surfactants and subsurface remediation , 1992 .

[14]  T. Imanaka,et al.  A study on the structure-function relationship of lipopeptide biosurfactants. , 2000, Biochimica et biophysica acta.

[15]  Ibrahim M. Banat,et al.  The isolation of a thermophilic biosurfactant producing Bacillus SP , 1993, Biotechnology Letters.

[16]  M. Amoozegar,et al.  Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery , 2008, Biotechnology Letters.

[17]  Ibrahim M. Banat,et al.  Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: a review , 1995 .

[18]  N. Youssef,et al.  Microbial processes in oil fields: culprits, problems, and opportunities. , 2009, Advances in applied microbiology.

[19]  Noha H. Youssef,et al.  Chapter 6 Microbial Processes in Oil Fields , 2009 .

[20]  Sanket J. Joshi,et al.  Biosurfactant production using molasses and whey under thermophilic conditions. , 2008, Bioresource technology.

[21]  M. M. Assadi,et al.  Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant , 2004 .

[22]  R. S. Bryant,et al.  Review of microbial technology for improving oil recovery , 1989 .

[23]  Changkai Zhang,et al.  Application of microbial enhanced oil recovery technique to Daqing Oilfield , 2002 .

[24]  N. Youssef,et al.  Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity , 2005, Applied and Environmental Microbiology.