Immunomagnetic reduction assay using high-Tc superconducting-quantum-interference-device-based magnetosusceptometry

Via immunomagnetic reduction assay, biomolecules can be quantitatively detected with aid of biofunctionalized magnetic nanoparticles, which are used as labeling markers for specific biomolecules. To achieve ultra-high sensitivity in detecting biomolecules, superconducting quantum interference device (SQUID) is a promising candidate to act as a sensor to the magnetic signal related to the concentration of detected biomolecules. In the past, we developed a single channel SQUID-based magnetosusceptometry. In order to increase the detection through-put, multichannel SQUID-based magnetosusceptometry is developed. In this work, the design and working principle of four-channel SQUID-based magnetosusceptometry are introduced. Using utilizing scanning technology, four samples can be simultaneously logged into the SQUID-based magnetosusceptometry. Notably, only single SQUID magnetometer is used in the magnetosusceptometry. The precision and sensitivity in detecting biomolecules using the four-channel SQUID-based ma...

[1]  Shieh-Yueh Yang,et al.  Hyper-high-sensitivity wash-free magnetoreduction assay on biomolecules using high- Tc superconducting quantum interference devices , 2008 .

[2]  Stephen E. Robinson,et al.  SQUID sensor array configurations for magnetoencephalography applications , 2002 .

[3]  Keiji Enpuku,et al.  Detection of Magnetic Nanoparticles with Superconducting Quantum Interference Device (SQUID) Magnetometer and Application to Immunoassays , 1999 .

[4]  W. Shimizu,et al.  Magnetocardiography Study on Ventricular Depolarization‐Current Pattern in Patients with Brugada Syndrome and Complete Right‐Bundle Branch Blocks , 2006, Pacing and clinical electrophysiology : PACE.

[5]  Shieh-Yueh Yang,et al.  Magnetic relaxation measurement in immunoassay using high-transition-temperature superconducting quantum interference device system , 2006 .

[6]  Shieh-Yueh Yang,et al.  Enhancement in low field nuclear magnetic resonance with a high-Tc superconducting quantum interference device and hyperpolarized H3e , 2008 .

[7]  Y. H. Lee,et al.  Two-dimensional propagation of magnetocardiac T wave signals for characterizing myocardial ischemia , 2008 .

[8]  J. Clarke,et al.  SQUID detected NMR of laser-polarized xenon at 4.2 K and at frequencies down to 200 Hz , 1997 .

[9]  C. Hong,et al.  Nanomagnetic particles for SQUID-based magnetically labeled immunoassay , 2005, IEEE Transactions on Applied Superconductivity.

[10]  Lutz Trahms,et al.  Determination of the binding reaction between avidin and biotin by relaxation measurements of magnetic nanoparticles , 1999 .

[11]  Yong-Sheng Chen,et al.  ICA-based spatiotemporal approach for single-trial analysis of postmovement MEG beta synchronization☆ , 2003, NeuroImage.