Repair of rabbit articular surfaces with allograft chondrocytes embedded in collagen gel.

In an attempt to repair articular cartilage, allograft articular chondrocytes embedded in collagen gel, were transplanted into full-thickness defects in rabbit articular cartilage. Twenty-four weeks after the transplantation, the defects were filled with hyaline cartilage, specifically synthesising Type II collagen. These chondrocytes were autoradiographically proven to have originated from the transplanted grafts. Assessed histologically the success rate was about 80%, a marked improvement over the results reported in previous studies on chondrocyte transplantation without collagen gel. By contrast, the defects without chondrocyte transplantation healed with fibrocartilage. Immunological enhancement induced by transplanted allogenic chondrocytes or collagen was not significant at eight weeks after treatment, so far as shown by both direct and indirect blastformation reactions. Thus, allogenic transplantation of isolated chondrocytes embedded in collagen gel appears to be one of the most promising methods for the restoration of articular cartilage.

[1]  Marc S. Lewis,et al.  The Chromatographic Separation and Amino Acid Composition of the Subunits of Several Collagens , 1963 .

[2]  Audrey U. Smith.  Survival of Frozen Chondrocytes Isolated from Cartilage of Adult Mammals , 1965, Nature.

[3]  H. Coon.  Clonal stability and phenotypic expression of chick cartilage cells in vitro. , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Abbott,et al.  THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS , 1969, The Journal of experimental medicine.

[5]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .

[6]  G. Bentley,et al.  Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits , 1971, Nature.

[7]  E. J. Miller,et al.  Identification of three genetically distinct collagens by cyanogen bromide cleavage of insoluble human skin and cartilage collagen. , 1971, Biochemical and biophysical research communications.

[8]  W. Bonner,et al.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. , 1974, European journal of biochemistry.

[9]  M. Elves.  A study of the transplantation antigens on chondrocytes from articular cartilage. , 1974, The Journal of bone and joint surgery. British volume.

[10]  A. Gross,et al.  Immunogenicity of allograft articular cartilage. , 1974, The Journal of bone and joint surgery. American volume.

[11]  E. J. Miller,et al.  Changes in type of collagen synthesized as clones of chick chondrocytes grow and eventually lose division capacity. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[12]  W. T. Green.  Articular cartilage repair. Behavior of rabbit chondrocytes during tissue culture and subsequent allografting. , 1977, Clinical orthopaedics and related research.

[13]  P. Benya,et al.  The progeny of rabbit articular chondrocytes synthesize collagen types I and III and type I trimer, but not type II. Verifications by cyanogen bromide peptide analysis. , 1977, Biochemistry.

[14]  M. Glimcher,et al.  Biochemical studies on repair cartilage resurfacing experimental defects in the rabbit knee. , 1980, The Journal of bone and joint surgery. American volume.

[15]  K. Ono,et al.  Primary culture of chondrocytes embedded in collagen gels. , 1982, Experimental cell biology.

[16]  P. Lipsky,et al.  Immunomodulatory effect of procainamide in man. Inhibition of human suppressor T-cell activity in vitro. , 1983, The Journal of clinical investigation.

[17]  K. Ono,et al.  Biosynthesis of type IX collagen during chick limb development. , 1985, Biochemical and biophysical research communications.

[18]  木村 友厚.  Chondrocytes embedded in collagen gels maintain cartilage phenotype during long-term cultures , 1985 .

[19]  G. Bentley,et al.  Repair of articular surfaces by allografts of articular and growth-plate cartilage. , 1986, The Journal of bone and joint surgery. British volume.

[20]  A. Abramovici,et al.  Use of cultured embryonal chick epiphyseal chondrocytes as grafts for defects in chick articular cartilage. , 1987, Clinical orthopaedics and related research.