A new animal model for human respiratory tract disease due to adenovirus.

Cotton rats (Sigmodon hispidus) were tested as a model for human respiratory tract infection due to adenovirus. After intranasal instillation of 10(6.1) 50% tissue culture infectious doses (TCID50) of adenovirus type 5 into one-month-old cotton rats, groups were killed at intervals for nasal and lung titration of virus and lung histopathology. In lung, eclipse occurred at 8 hr followed by peak viral titer (10(7.5) TCID50/g of lung) on day 5. Titers fell to 10(3.2) TCID50/g by day 10 and persisted at that level through the remainder of the study (day 28) despite appearance of serum neutralizing antibody after day 6. Interstitial pneumonia paralleled viral growth, and peribronchial mononuclear infiltration followed one to two days later. Titers in nasal mucosa peaked on day 3 but were undetectable beyond day 21. Pulmonary histopathology and viral replicative patterns paralleled findings in natural human disease.

[1]  R. Chanock,et al.  The pathogenesis of respiratory syncytial virus infection in cotton rats. , 1978, The American journal of pathology.

[2]  J. Trentin,et al.  Oncogenic Effect of Human Adenovirus Type 12, in Mice , 1964, Science.

[3]  R. Postlethwaite.  Liver Damage Induced in Mice by Human Adenovirus Type 5 , 1973 .

[4]  M. Cooney,et al.  Epidemiology of viral and mycoplasmal agents associated with childhood lower respiratory illness in a civilian population. , 1971, The Journal of pediatrics.

[5]  R. Chanock,et al.  Infections in 18,000 infants and children in a controlled study of respiratory tract disease. I. Adenovirus pathogenicity in relation to serologic type and illness syndrome. , 1969, American journal of epidemiology.

[6]  D. Porter,et al.  Adenovirus hepatitis in an immunosuppressed adult patient. , 1979, American journal of clinical pathology.

[7]  J. Beckwith,et al.  A FATAL CASE OF INCLUSION BODY PNEUMONIA IN AN INFANT INFECTED WITH ADENOVIRUS TYPE 3. , 1964, The Journal of pediatrics.

[8]  G. R. French,et al.  Fatal pneumonia associated with adenovirus type 7 in three military trainees. , 1972, The New England journal of medicine.

[9]  H. Pereira,et al.  Fatal Infection of New-Born Hamsters by an Adenovirus of Human Origin , 1962, Nature.

[10]  A. R. Jennings,et al.  Inoculation of Pigs with Adenoviruses of Man , 1962, Nature.

[11]  D. Porter,et al.  Adenovirus infection in the immunocompromised patient. , 1980, The American journal of medicine.

[12]  Y. Chardonnet,et al.  Persistence of Adenovirus 5 in Guinea Pigs , 1974, Infection and immunity.

[13]  W. Andiman,et al.  Persistent infection with adenovirus types 5 and 6 in lymphoid cells from humans and woolly monkeys. , 1982, The Journal of infectious diseases.

[14]  L. Reed,et al.  A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS , 1938 .

[15]  R. Huebner,et al.  STUDIES OF THE ADENOIDAL-PHARYNGEAL-CONJUNCTI-VAL (APC) GROUP OF VIRUSES , 1955 .

[16]  M. D. Eaton,et al.  AN INFECTIOUS AGENT FROM CASES OF ATYPICAL PNEUMONIA APPARENTLY TRANSMISSIBLE TO COTTON RATS. , 1942, Science.

[17]  J. Fox,et al.  THE VIRUS WATCH PROGRAM: A CONTINUING SURVEILLANCE OF VIRAL INFECTIONS IN METROPOLITAN NEW YORK FAMILIESI. OVERALL PLAN, METHODS OF COLLECTING AND HANDLING INFORMATION AND A SUMMARY REPORT OF SPECIMENS COLLECTED AND ILLNESSES OBSERVED , 1966 .

[18]  J. Trentin,et al.  The Quest for Human Cancer Viruses , 1962, Science.

[19]  J. Fox,et al.  The Seattle Virus Watch. VII. Observations of adenovirus infections. , 1977, American journal of epidemiology.

[20]  W. S. Jordan,et al.  A study of illness in a group of Cleveland families. XV. Acquisition of type-specific adenovirus antibodies in the first five years of life; implications for the use of adenovirus vaccine. , 1958, The New England journal of medicine.

[21]  H. Pinkerton,et al.  Fatal adenovirus pneumonia in infants. Correlation of histologic and electron microscopic observations. , 1971, The American journal of pathology.

[22]  L. Elveback,et al.  The virus watch program: a continuing surveillance of viral infections in metropolitan New York families. VI. Observations of adenovirus infections: virus excretion patterns, antibody response, efficiency of surveillance, patterns of infections, and relation to illness. , 1969, American journal of epidemiology.

[23]  S. Scholes,et al.  Current considerations in public health of the role of animals in relation to human viral diseases. , 1960, Journal of the American Veterinary Medical Association.

[24]  E. Dubovi,et al.  The cotton rat as an experimental model of human parainfluenza virus type 3 disease. , 1981, Experimental lung research.

[25]  J. Connor,et al.  Neonatal infection caused by adenovirus type 7. , 1968, The Journal of pediatrics.

[26]  J. Tooze.  The molecular biology of tumour viruses , 1973 .

[27]  H. Wigger,et al.  Fatal hepatic and bronchial necrosis in adenovirus infection with thymic alymphoplasia. , 1966, The New England journal of medicine.

[28]  D. Becroft.  Histopathology of fatal adenovirus infection of the respiratory tract in young children , 1967, Journal of clinical pathology.