Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.)

Abstract The effect of sodium chloride (NaCl) concentrations (0.0, 60, 120, 240 mM) on growth, osmotic potential, chlorophyll content, protein content of ( Vicia faba L.) seedlings was investigated. NaCl caused an increase in plant height with low and medium concentrations and a decrease with the highest concentration, in both measurement periods. No significant effect was observed in the number of leaves or leaf area with low concentration, while a decrease was noticed for each, with two higher concentrations and in both measurement periods. Salinity increased both fresh and dry weights of the shoot in the two measurement periods. Osmotic potential (O.P.) showed a significant decrease with the increase in concentrations, and in the duration of the stress periods. Salinity significantly reduced chlorophyll ‘a’ content in both measurement periods. It also significantly reduced chlorophyll ‘b’, total chl., and carotenoids contents after ten days of treatment. An increase was observed in the protein content in the two measurement periods due to the impact of salinity stress. A directly proportional relationship was found between protein content and the increase in salt concentrations in the first measurement period, while it was inversely proportional in the second.

[1]  Joginder Singh,et al.  Biomass Production, Productivity and Physiological Changes in Moth Bean Genotypes at Different Salinity Levels , 2006 .

[2]  K. Tanaka,et al.  Physiological response of common bean (Phaseolus vulgaris L.) seedlings to salinity stress , 2007 .

[3]  C. Poschenrieder,et al.  Efficient leaf ion partitioning, an overriding condition for abscisic acid-controlled stomatal and leaf growth responses to NaCl salinization in two legumes. , 2003, Journal of experimental botany.

[4]  E. Beck,et al.  Sorghum and Salinity , 2004 .

[5]  R. Munns.  Comparative physiology of salt and water stress. , 2002, Plant, cell & environment.

[6]  E. Ogie-odia.  Salinity Effects on Young Healthy Seedlings of Kyllingia Peruviana Collected from Escravos, Delta State , 2008 .

[7]  L. Rui,et al.  Leaf anatomical changes of Bruguiera gymnorrhiza seedlings under salt stress. , 2009 .

[8]  V. D. Taffouo,et al.  Effects of salinity stress on growth, ions partitioning and yield of some cowpea (Vigna unguiculata L. Walp.) cultivars. , 2009 .

[9]  H. Nguyen,et al.  Genetic analysis of osmotic adjustment in crop plants , 1999 .

[10]  C. A. Jaleel,et al.  Interactive effects of triadimefon and salt stress on antioxidative status and ajmalicine accumulation in Catharanthus roseus , 2008, Acta Physiologiae Plantarum.

[11]  S. Schubert,et al.  Salt-resistant and salt-sensitive wheat genotypes show similar biochemical reaction at protein level in the first phase of salt stress , 2006 .

[12]  Effect of salinity on organic solutes contents in barley. , 2009, Pakistan journal of biological sciences : PJBS.

[13]  S. Réhman,et al.  SALINITY EFFECT ON PLANT GROWTH, PSII PHOTOCHEMISTRY AND CHLOROPHYLL CONTENT IN SUGAR BEET (BETA VULGARIS L.) AND CABBAGE (BRASSICA OLERACEA CAPITATA L.) , 2007 .

[14]  N. Sultana,et al.  Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains , 1999 .

[15]  V. D. Taffouo,et al.  Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranea (L.) Verdc.) landraces grown under saline conditions. , 2010 .

[16]  A. N. Misra,et al.  The xanthophyll cycle activity in kidney bean and cabbage leaves under salinity stress , 2006, Russian Journal of Plant Physiology.

[17]  A. Srivastava,et al.  Assessment of salinity tolerance of Vigna mungo var. Pu-19 using ex vitro and in vitro methods. , 2010 .

[18]  B. F. Dantas,et al.  Resposta fisiológica de sementes de feijão caupi ao estresse salino , 2005 .

[19]  J. Alarcón,et al.  Effects of salinity on growth, shoot water relations and root hydraulic conductivity in tomato plants , 1997, The Journal of Agricultural Science.

[20]  S. Réhman,et al.  Salinity reduced growth PS2 photochemistry and chlorophyll content in radish , 2007 .

[21]  Kiyoshi Tanaka,et al.  Salt-Induced Stress Effects on Biomass, Photosynthetic Rate, and Reactive Oxygen Species-Scavenging Enzyme Accumulation in Common Bean , 2009 .

[22]  J. L. Andriolo,et al.  Growth and yield of lettuce plants under salinity , 2005 .

[23]  S. A. Memon,et al.  Morphological analysis of salt stress response of pak choi. , 2010 .

[24]  H. Bohnert,et al.  PLANT CELLULAR AND MOLECULAR RESPONSES TO HIGH SALINITY. , 2000, Annual review of plant physiology and plant molecular biology.

[25]  S. Yamada,et al.  Influence of Calcium Silicate on Growth, Physiological Parameters and Mineral Nutrition in Two Legume Species Under Salt Stress , 2007 .

[26]  Samia El-Sayed Saffan.  Effect of Salinity and Osmotic Stresses on Some Economic Plants , 2008 .

[27]  B. Etherton.  Relationship of Cell Transmembrane Electropotential to Potassium and Sodium Accumulation Ratios in Oat and Pea Seedlings. , 1963, Plant physiology.

[28]  T. Cvetić,et al.  EFFECTS OF SALINITY ON IN VITRO GROWTH AND PHOTOSYNTHESIS OF COMMON CENTAURY (CENTAURIUM ERYTHRAEA RAFN.) , 2007 .

[29]  B. Sanwal,et al.  MODERN METHODS OF PLANT ANALYSIS , 1955 .

[30]  Yi Ding,et al.  Genetic analysis of salt stress responses in asparagus bean (Vigna unguiculata (L.) ssp. sesquipedalis Verdc.). , 2007, The Journal of heredity.

[31]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[32]  J. Zhu,et al.  Plant salt tolerance. , 2001, Trends in plant science.

[33]  F. N. David,et al.  Principles and procedures of statistics. , 1961 .

[34]  S. Renault,et al.  Response of red-osier dogwood (Cornus sericea) seedlings to NaCl during the onset of bud break , 2006 .

[35]  A. N. Misra,et al.  Sodium chloride induced changes in leaf growth, and pigment and protein contents in two rice cultivars , 1997, Biologia Plantarum.

[36]  J. Lynch,et al.  Salinity Tolerance in Phaseolus Species during Early Vegetative Growth , 2002 .

[37]  Zhanghua Wang,et al.  Short-term responses to salinity of seabuckthorn (Hippophae rhamnoides L.) seedlings in the extremely cold and saline Qinghai region of China , 2009 .

[38]  Geung-Joo Lee,et al.  Photosynthetic responses to salinity stress of halophytic seashore paspalum ecotypes , 2004 .

[39]  M. Shannon.  New Insights in Plant Breeding Efforts for Improved Salt Tolerance , 1996 .

[40]  A. Yeo,et al.  Molecular biology of salt tolerance in the context of whole-plant physiology , 1998 .

[41]  S. Réhman,et al.  SALINITY (NACL) TOLERANCE OF BRASSICA SPECIES AT GERMINATION AND EARLY SEEDLING GROWTH , 2005 .

[42]  A. D. Day,et al.  Effects of Soil‐Moisture Regimes on the Growth of Barley1 , 1975 .

[43]  Gu,et al.  Leucine aminopeptidase RNAs, proteins, and activities increase in response to water deficit, salinity, and the wound signals systemin, methyl jasmonate, and abscisic acid , 1999, Plant physiology.

[44]  B. Ma,et al.  Growth, Gas Exchange, Chlorophyll Fluorescence, and Ion Content of Naked Oat in Response to Salinity , 2007 .

[45]  R. Munns.  Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses , 1993 .

[46]  James H. C. Smith,et al.  Chlorophylls: Analysis in Plant Materials , 1955 .

[47]  P. M. Neumann.  Salinity resistance and plant growth revisited , 1997 .

[48]  H. Schreiber,et al.  Barley Production as Influenced by Timing of Soil Moisture and Timing of Nitrogen Applications1 , 1965 .

[49]  T. Flowers,et al.  Effects of salinity and ozone, individually and in combination, on the growth and ion contents of two chickpea (Cicer arietinum L.) varieties. , 2002, Environmental pollution.

[50]  E. Troyo-Diéguez,et al.  Response to salinity of three grain legumes for potential cultivation in arid areas , 2003 .