Purification of antibodies by affinity chromatography.

This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. Affinity techniques allow antibody purification both in a single step chromatographic procedure as well as in complex purification protocols depending on the intention to use the target antibody. The purification strategies for antibodies by interaction with affinity ligands such as antibodies and Fe receptors or low molecular weight compounds are described.

[1]  G. Fassina,et al.  Protein a mimetic peptide ligand for affinity purification of antibodies , 1996, Journal of molecular recognition : JMR.

[2]  J J Valdes,et al.  Recombinant antibodies: a new reagent for biological agent detection. , 2000, Biosensors & bioelectronics.

[3]  M Vanderlaan,et al.  Performance comparison of Protein A affinity‐chromatography sorbents for purifying recombinant monoclonal antibodies , 1999, Biotechnology and applied biochemistry.

[4]  J. Porath.  Immobilized metal ion affinity chromatography. , 1992, Protein expression and purification.

[5]  K. Huse,et al.  Salt-independent binding of antibodies from human serum to thiophilic heterocyclic ligands. , 1998, Journal of chromatography. B, Biomedical sciences and applications.

[6]  J. Porath,et al.  Rapid one-step purification of goat immunoglobulins by immobilized metal ion affinity chromatography. , 1995, Journal of immunological methods.

[7]  S Gülich.  Protein engineering of an IgG-binding domain allows milder elution conditions during affinity chromatography , 2000 .

[8]  M. Boyle,et al.  Analysis of immunoglobulin G-binding-protein expression by invasive isolates of Streptococcus pyogenes , 1995, Clinical and diagnostic laboratory immunology.

[9]  E. Coligan.  Current protocols in immunology , 1991 .

[10]  P. Berna,et al.  Comparison of the protein adsorption selectivity of salt-promoted agarose-based adsorbents. Hydrophobic, thiophilic and electron donor-acceptor adsorbents. , 1998, Journal of chromatography. A.

[11]  K. Huse,et al.  A simplified procedure for the isolation of immunoglobulins from human serum using a novel type of thiophilic gel at low salt concentration. , 1998, Journal of immunological methods.

[12]  G. Clofent-Sanchez,et al.  Three-step purification of bacterially expressed human single-chain Fv antibodies for clinical applications. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[13]  J. Porath,et al.  Covalent chromatography and salt-promoted thiophilic adsorption. , 1989, Analytical Biochemistry.

[14]  Langone Jj.  Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci. , 1982 .

[15]  G. Fassina.  Protein A Mimetic (PAM) Affinity Chromatography , 2000 .

[16]  S. Yoshida,et al.  Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes. , 2001, Molecular and biochemical parasitology.

[17]  M. Boyle,et al.  Distinct profiles of immunoglobulin G-binding-protein expression by invasive serotype M1 isolates of Streptococcus pyogenes , 1995, Clinical and diagnostic laboratory immunology.

[18]  M. Uhlén,et al.  Mutational analysis of the interaction between staphylococcal protein A and human IgG1. , 1993, Protein engineering.

[19]  G. Fassina,et al.  IRIS 97: an innovative protein A-peptidomimetic solid phase medium for antibody purification. , 1998, Journal of molecular recognition : JMR.

[20]  J. Porath,et al.  Synthesis of new hydrophobic adsorbents based on homologous series of uncharged alkyl sulphide agarose derivatives. , 1985, Journal of chromatography.

[21]  S. Schillberg,et al.  Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[22]  M. Tortora,et al.  A synthetic ligand for IgA affinity purification , 1998, Journal of molecular recognition : JMR.

[23]  Robertson Er,et al.  Glycoproteins: a consideration of the potential problems and their solutions with respect to purification and characterisation. , 1996 .

[24]  M. Caron,et al.  Use of thiophilic adsorption in the purification of biotinylated Fab fragments. , 1995, Journal of chromatography. B, Biomedical applications.

[25]  F. Simons,et al.  Binding of dog immunoglobulins G, A, M, and E to concanavalin A. , 1993, Veterinary immunology and immunopathology.

[26]  Y. Clonis,et al.  Design and application of bio-mimetic dyes in biotechnology. , 1986, Journal of chromatography.

[27]  T. Phillips,et al.  Isolation and quantitation of serum IgE levels by high-performance immunoaffinity chromatography. , 1985, Journal of chromatography.

[28]  J. J. Langone.  Applications of immobilized protein A in immunochemical techniques. , 1982, Journal of immunological methods.

[29]  D. Harding,et al.  Hydrophobic charge induction chromatography: salt independent protein adsorption and facile elution with aqueous buffers. , 1998, Journal of chromatography. A.

[30]  M. Hearn,et al.  Adsorption and selectivity characteristics of several human serum proteins with immobilised hard Lewis metal ion-chelate adsorbents. , 2000, Journal of chromatography. A.

[31]  P. Blond,et al.  Purification of immunoglobulins G by protein A/G affinity membrane chromatography. , 1999, Journal of chromatography. B, Biomedical sciences and applications.

[32]  G. Fassina,et al.  Affinity purification of mouse monoclonal IgE using a protein A mimetic ligand (TG19318) immobilized on solid supports , 1998, Journal of molecular recognition : JMR.

[33]  R. Reeves,et al.  HMGI/Y proteins: flexible regulators of transcription and chromatin structure. , 2001, Biochimica et biophysica acta.

[34]  J. Goudswaard,et al.  Protein A Reactivity of Various Mammalian Immunoglobulins , 1978, Scandinavian journal of immunology.

[35]  M. Radic,et al.  Tandem affinity tags for the purification of bivalent anti-DNA single-chain Fv expressed in Escherichia coli. , 1999, Protein expression and purification.

[36]  E. R. Robertson,et al.  Glycoproteins: a consideration of the potential problems and their solutions with respect to purification and characterisation. , 1996, Bioseparation.

[37]  S. Batra,et al.  Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct. , 2000, Biochimica et biophysica acta.

[38]  I. Smirnov,et al.  DNA hydrolyzing autoantibodies. , 1992, Science.

[39]  A. Schots,et al.  Fluobodies: green fluorescent single-chain Fv fusion proteins. , 1999, Journal of immunological methods.

[40]  R. Clift,et al.  A sensitive enzyme-linked immunosorbent assay (ELISA) for the detection of staphylococcal protein A (SpA) present as a trace contaminant of murine immunoglobulins purified on immobilized protein A. , 1992, Journal of immunological methods.

[41]  S. Ohlson,et al.  Affinity screening for weak monoclonal antibodies. , 1998, Journal of immunological methods.

[42]  J. Deisenhofer.  Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution. , 1981, Biochemistry.

[43]  G. Hale,et al.  Repeated cleaning of protein A affinity column with sodium hydroxide. , 1994, Journal of immunological methods.

[44]  G. Fassina,et al.  Affinity purification of immunoglobulin M using a novel synthetic ligand. , 1998, Journal of chromatography. B, Biomedical sciences and applications.

[45]  R. Lindmark,et al.  Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera. , 1983, Journal of immunological methods.

[46]  N. Kruger,et al.  Purification of immunoglobulins using protein a-sepharose. , 1988, Methods in molecular biology.

[47]  G. Fassina,et al.  Affinity purification of immunoglobulins from chicken egg yolk using a new synthetic ligand. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[48]  M. Roque-Barreira,et al.  Jacalin: an IgA-binding lectin. , 1985, Journal of immunology.

[49]  A. Lihme,et al.  Protein A mimetic for large-scale monoclonal antibody purification , 1997 .

[50]  J. Porath,et al.  Thiophilic adsorption ‐ a new method for protein fractionation , 1985, FEBS letters.

[51]  S. Kabir.  Jacalin: a jackfruit (Artocarpus heterophyllus) seed-derived lectin of versatile applications in immunobiological research. , 1998, Journal of immunological methods.

[52]  T. Arakawa,et al.  Expression, purification, and characterization of the active immunoglobulin-like domain of human granulocyte-colony-stimulating factor receptor in Escherichia coli. , 2001, Protein expression and purification.

[53]  M. Vijayalakshmi,et al.  A preliminary study for isolation of catalytic antibodies by histidine ligand affinity chromatography as an alternative to conventional protein A/G methods , 2000, Applied biochemistry and biotechnology.

[54]  C. Lowe,et al.  Design, synthesis, and application of a Protein A mimetic , 1998, Nature Biotechnology.

[55]  C. Lowe,et al.  Affinity chromatography on immobilized "biomimetic" ligands. Synthesis, immobilization and chromatographic assessment of an immunoglobulin G-binding ligand. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[56]  J. Hale,et al.  Purification of humanized murine and murine monoclonal antibodies using immobilized metal-affinity chromatography. , 1994, Analytical biochemistry.

[57]  H. Zola,et al.  Separation of IgE from IgG subclasses using staphylococcal protein A. , 1978, International archives of allergy and applied immunology.

[58]  J. Porath,et al.  A one-step purification method for monoclonal antibodies based on salt-promoted adsorption chromatography on a 'thiophilic' adsorbent. , 1987, Journal of immunological methods.

[59]  G. Fassina,et al.  Immunoglobulin specificity of TG19318: a novel synthetic ligand for antibody affinity purification , 1998, Journal of molecular recognition : JMR.

[60]  Y. Kihira,et al.  Artificial immunoglobulin G-binding protein mimetic to staphylococcal protein A. Its production and application to affinity purification of immunoglobulin G. , 1992, Journal of chromatography.

[61]  N. Rose,et al.  Autoantibodies to thyroglobulin in health and disease , 2000, Applied biochemistry and biotechnology.

[62]  R L Fahrner,et al.  Expanded bed protein A affinity chromatography of a recombinant humanized monoclonal antibody: process development, operation, and comparison with a packed bed method. , 1999, Journal of biotechnology.

[63]  N. Sheibani.  Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins. , 1999, Preparative biochemistry & biotechnology.

[64]  A. Subramanian.  Immunoaffinity chromatography , 2000, Methods in molecular biology.

[65]  M. Roque-Barreira,et al.  IgA-affinity purification and characterization of the lectin jacalin. , 1986, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[66]  A. Forsgren,et al.  “Protein A” from S. Aureus I. Pseudo-Immune Reaction with Human γ-Globulin , 1966 .

[67]  A. Skerra,et al.  Use of thiophilic adsorption chromatography for the one-step purification of a bacterially produced antibody F(ab) fragment without the need for an affinity tag. , 1999, Protein expression and purification.

[68]  V. Marks,et al.  Assessment of the suitability of commercially available SpA affinity solid phases for the purification of murine monoclonal antibodies at process scale. , 1993, Journal of immunological methods.