Parasitism in Kansas in the 1800s: a glimpse to the past through the analysis of grave sediments from Meadowlark cemetery.

During the excavations of the XIX century Meadowlark cemetery (Manhattan, Kansas, US), samples of sediments were taken from around five skeletons, and analyzed to detect intestinal parasites. No helminth eggs were found, but immunological ELISA tests for Entamoeba histolytica were positive in three samples. The immunological techniques have been successfully used in paleoparasitology to detect protozoan infections. Amoebiasis could have been a severe disease in the past, especially where poor sanitary conditions prevailed, and there is evidence that this cemetery may have been used in a situation where poor sanitary conditions may have prevailed. The presence of this protozoan in US during the late XIX century gives information on the health of the population and provides additional data on the parasite's evolution since its appearance in the New World.

[1]  M. Le Bailly,et al.  Diphyllobothrium: Neolithic Parasite? , 2005, The Journal of parasitology.

[2]  K. Reinhard,et al.  Amoebiasis distribution in the past: first steps using an immunoassay technique. , 2004, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[3]  M. Le Bailly,et al.  Dioctophymidae Eggs in Coprolites From Neolithic Site of Arbon–Bleiche 3 (Switzerland) , 2003, The Journal of parasitology.

[4]  Y. Ortega,et al.  Cryptosporidium, Giardia, and Cyclospora in Ancient Peruvians , 2003, The Journal of parasitology.

[5]  F. Bouchet,et al.  A l'interface de la biologie et de l'archéologie : la Paléoparasitologie. , 2003 .

[6]  M. Le Bailly,et al.  The state of the art of paleoparasitological research in the Old World. , 2003, Memorias do Instituto Oswaldo Cruz.

[7]  L. F. Ferreira,et al.  Human intestinal parasites in the past: new findings and a review. , 2003, Memorias do Instituto Oswaldo Cruz.

[8]  K. Dittmar,et al.  Parasite remains in archaeological sites. , 2003, Memorias do Instituto Oswaldo Cruz.

[9]  K. Reinhard,et al.  Detection of Giardia duodenalis antigen in coprolites using a commercially available enzyme-linked immunosorbent assay. , 2002, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[10]  L. F. Ferreira,et al.  First Recovery of Schistosoma mansoni Eggs From a Latrine in Europe (15–16th Centuries) , 2002, The Journal of parasitology.

[11]  C. Lefèvre,et al.  Identification of parasitoses in a child burial from Adak Island (Central Aleutian Islands, Alaska). , 2001, Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie.

[12]  K. Reinhard,et al.  American hookworm antiquity , 2001, Medical anthropology.

[13]  R. Haque,et al.  Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests , 2000, Journal of Clinical Microbiology.

[14]  C. Huston,et al.  Molecular-based diagnosis of Entamoeba histolytica infection , 1999, Expert Reviews in Molecular Medicine.

[15]  T. Nash,et al.  TechLab and Alexon Giardia Enzyme-Linked Immunosorbent Assay Kits Detect Cyst Wall Protein 1 , 1999, Journal of Clinical Microbiology.

[16]  R. Jantz.  Bioarchaeology: Interpreting behavior from the human skeleton , 1999 .

[17]  A. Vicente,et al.  Paleoparasitology: perspectives with new techniques. , 1998, Revista do Instituto de Medicina Tropical de Sao Paulo.

[18]  D. Hale,et al.  Comparison of Nine Commercially Available Enzyme-Linked Immunosorbent Assays for Detection of Giardia lamblia in Fecal Specimens , 1998, Journal of Clinical Microbiology.

[19]  R. Haque,et al.  Comparison of PCR, Isoenzyme Analysis, and Antigen Detection for Diagnosis of Entamoeba histolyticaInfection , 1998, Journal of Clinical Microbiology.

[20]  R. Hould.  Techniques d'histopathologie et de cytopathologie / , 1998 .

[21]  F. Bouchet.  Intestinal capillariasis in neolithic inhabitants of Chalain (Jura, France) , 1997, The Lancet.

[22]  F. Bouchet,et al.  Capillarioses intestinales : Nematodoses du neolithique? , 1997 .

[23]  D. Warhurst,et al.  Diagnosis of Entamoeba histolytica and Entamoeba dispar in clinical specimens by PCR-SHELA. , 1997, Archives of medical research.

[24]  R. Haque,et al.  Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits , 1995, Journal of clinical microbiology.

[25]  F. Guhl,et al.  Polymerase chain reaction-solution hybridization enzyme-linked immunoassay (PCR-SHELA) for the differential diagnosis of pathogenic and non-pathogenic Entamoeba histolytica. , 1995, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[26]  C. Clark,et al.  A Redescription of Entamoeba Histolytica Schaudinn, 1903 (Emended Walker, 1911) Separating It From Entamoeba Dispar Brumpt, 1925 1 , 1993, The Journal of eukaryotic microbiology.

[27]  K. Reinhard.  Parasitology as an Interpretive Tool in Archaeology , 1992, American Antiquity.

[28]  Edward L. Bell.  The historical archaeology of mortuary behavior: Coffin hardware from Uxbridge, Massachusetts , 1990 .

[29]  A. Swedlund,et al.  Biocultural perspectives on stress in prehistoric, historical, and contemporary population research , 1988 .

[30]  K. Reinhard,et al.  Helminth remains from prehistoric Indian coprolites on the Colorado Plateau. , 1987, The Journal of parasitology.