Extraordinary transmission-based super-resolved axial imaging using subwavelength metallic nanoaperture arrays

A super-resolved axial imaging technique was investigated based on extraordinary transmission (EOT) of light using metallic gradient nanoaperture arrays. Light through subwavelength nanoapertures at thick metal film can be transmitted and amplified by several orders of magnitude due to plasmonic coupling. Here, the feasibility of EOT-based axial imaging with super resolution is explored. Since light penetration of EOT is much deeper than that of evanescent waves, the axial range to obtain the distance information of fluorescence signals can be extended by EOT. The axial distribution of ganglioside in mouse macrophage cells was measured with sub-diffraction-limited resolution after reconstruction using differential fluorescence excitation on gradient aperture arrays.

[1]  M. Gustafsson.  Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[2]  H. Altug,et al.  An optofluidic nanoplasmonic biosensor for direct detection of live viruses from biological media. , 2010, Nano letters.

[3]  Kyujung Kim,et al.  Enhanced detection of virus particles by nanoisland-based localized surface plasmon resonance. , 2013, Biosensors & bioelectronics.

[4]  Kyujung Kim,et al.  Extraordinary Transmission‐based Plasmonic Nanoarrays for Axially Super‐Resolved Cell Imaging , 2014 .

[5]  Kyujung Kim,et al.  Plasmonics-based spatially activated light microscopy for super-resolution imaging of molecular fluorescence. , 2010, Optics letters.

[6]  H. Lezec,et al.  Extraordinary optical transmission through sub-wavelength hole arrays , 1998, Nature.

[7]  J. Lippincott-Schwartz,et al.  Imaging Intracellular Fluorescent Proteins at Nanometer Resolution , 2006, Science.

[8]  Donghyun Kim,et al.  Self-aligned colocalization of 3D plasmonic nanogap arrays for ultra-sensitive surface plasmon resonance detection. , 2014, Biosensors & bioelectronics.

[9]  Michael J Rust,et al.  Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) , 2006, Nature Methods.

[10]  Stefan W. Hell,et al.  Supporting Online Material Materials and Methods Figs. S1 to S9 Tables S1 and S2 References Video-rate Far-field Optical Nanoscopy Dissects Synaptic Vesicle Movement , 2022 .

[11]  Martin Dressel,et al.  Optical transmission through subwavelength hole arrays in ultrathin metal films , 2011 .

[12]  B. Ooi,et al.  Enhanced extraordinary optical transmission (EOT) through arrays of bridged nanohole pairs and their sensing applications. , 2014, Nanoscale.

[13]  Wonju Lee,et al.  Nanogap-based dielectric-specific colocalization for highly sensitive surface plasmon resonance detection of biotin-streptavidin interactions , 2012 .

[14]  Kyujung Kim,et al.  Nanoisland-based random activation of fluorescence for visualizing endocytotic internalization of adenovirus. , 2010, Small.

[15]  Kyujung Kim,et al.  Nanoscale localization sampling based on nanoantenna arrays for super-resolution imaging of fluorescent monomers on sliding microtubules. , 2012, Small.

[16]  Donghyun Kim,et al.  Surface plasmon-enhanced nanoscopy of intracellular cytoskeletal actin filaments using random nanodot arrays. , 2014, Optics express.

[17]  Kyujung Kim,et al.  Target-Localized Nanograting-Based Surface Plasmon Resonance Detection toward Label-free Molecular Biosensing , 2010, IEEE Journal of Selected Topics in Quantum Electronics.