Disposable electrochemical magneto immunosensor for simultaneous simplified calibration and determination of Ochratoxin A in coffee samples

Abstract In food producing and developing countries having a reliable and inexpensive tool for the analysis of agricultural commodities is essential. This article describes a reliable disposable electrochemical magnetoimmunosensor for monitoring of coffee samples for Ochratoxin A contamination. The electrochemical detection performed on carbon screen-printed electrodes (CSPEs) used magnetic beads as antibody immobilization support and enzyme horseradish peroxidase as tracer. A remarkable limit of detection of 0.32 μg L −1 , well below of the legislative requirements, demonstrated the suitability of the proposed immunosensor as a screening tool for the analysis of Ochratoxin A in coffee samples. A simultaneous simplified Ochratoxin A calibration and coffee sample analysis strategy allowed the accurate determination of the mycotoxin with recoveries of 90% reducing the long times used in conventional calibration. This strategy enhanced the analytical merits of immunosensor approach towards truly disposable tools for screening of Ochratoxin A in coffee samples and opens novel avenues for food-safety monitoring.

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