Topography of photosynthetic activity of leaves obtained from video images of chlorophyll fluorescence.

The distribution of photosynthetic activity over the area of a leaf and its change with time was determined (at low partial pressure of O(2)) by recording images of chlorophyll fluorescence during saturating light flashes. Simultaneously, the gas exchange was being measured. Reductions of local fluorescence intensity quantitatively displayed the extent of nonphotochemical quenching; quench coefficients, q(N), were computed pixel by pixel. Because rates of photosynthetic electron transport are positively correlated with (1 - q(N)), computed images of (1 - q(N)) represented topographies of photosynthetic activity. Following application of abscisic acid to the heterobaric leaves of Xanthium strumarium L., clearly delineated regions varying in nonphotochemical quenching appeared that coincided with areoles formed by minor veins and indicated stomatal closure in groups.

[1]  Ian T. Young,et al.  An Analysis Technique for Biological Shape. I , 1974, Inf. Control..

[2]  Lars Olof Björn,et al.  Imaging by delayed light-emission (phytoluminography) as a method for detecting damage to the photosynthetic system , 1979 .

[3]  N. Baker,et al.  Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of photosystem II electron acceptors and fluorescence emission from photosystems I and II. , 1981, Biochimica et biophysica acta.

[4]  R. Amundson,et al.  Delayed Light Imaging for the Early Detection of Plant Stress , 1982, Science.

[5]  Christopher B. Field,et al.  A portable system for measuring carbon dioxide and water vapour exchange of leaves , 1982 .

[6]  J. Briantais,et al.  Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components , 1982 .

[7]  P. Horton.  Control of chloroplast electron transport by phosphorylation of thylakoid proteins , 1983 .

[8]  M. Onoe,et al.  Image analysis of chlorophyll fluorescence transients for diagnosing the photosynthetic system of attached leaves. , 1987, Plant physiology.

[9]  R. Richards,et al.  Genetic Variation in the Relationship between Photosynthetic CO2 Assimilation Rate and Stomatal Conductance to Water Loss , 1987 .

[10]  Joseph A. Berry,et al.  Quantum efficiency of Photosystem II in relation to ‘energy’-dependent quenching of chlorophyll fluorescence , 1987 .

[11]  J. Berry,et al.  Photosynthetic Control of Electron Transport in Leaves of Phaseolus Vulgaris: Evidence for Regulation of Photosystem 2 by the Proton Gradient , 1987 .

[12]  I. E. Woodrow,et al.  Enzymatic Regulation of Photosynthetic CO2, Fixation in C3 Plants , 1988 .

[13]  K. Winter,et al.  Zeaxanthin and the Heat Dissipation of Excess Light Energy in Nerium oleander Exposed to a Combination of High Light and Water Stress. , 1988, Plant physiology.

[14]  B. Loveys,et al.  Stomatal closure fully accounts for the inhibition of photosynthesis by abscisic acid. , 1988, The New phytologist.

[15]  Peter Horton,et al.  Studies on the induction of chlorophyll fluorescence in isolated barley protoplasts. IV. Resolution of non-photochemical quenching , 1988 .

[16]  G. Farquhar,et al.  Characterisation of Non-Uniform Photosynthesis Induced by Abscisic Acid in Leaves Having Different Mesophyll Anatomies , 1988 .

[17]  K. Raschke.  How abscisic acid causes depressions of the photosynthetic capacity of leaves. , 1990 .