Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection.

VCAM-1, a leukocyte adhesion molecule expressed by cytokine-activated endothelial cells in culture, may mediate mononuclear leukocyte infiltration in vessels and interstitium in solid organ allograft rejection. Using the avidin-biotin immunoperoxidase technique and two different antibodies--4B9, a murine monoclonal antibody, and rabbit polyclonal antisera to recombinant human VCAM (rVCAM Ab), which work in methacarn-fixed tissues--we studied the expression of this molecule in biopsies of transplanted liver and pancreas with and without features of rejection as well as nontransplant control tissues. The rVCAM Ab, but not 4B9, showed a population of reactive endothelial cells limited to sites of prominent subendothelial leukocytic cell infiltration in arteries and veins in rejecting allografts. VCAM-1 expression by sinusoidal endothelium in rejecting liver allografts was also observed. In addition, a population of cells (DC) with dendritic morphology was identified by rVCAM Ab within sites of lymphoid cell aggregations in both liver and pancreas allografts. Further evidence that these cells represent true DC was obtained by identification of VCAM-1 positive morphologically similar cells in both germinal centers and interfollicular areas of reactive lymph nodes; and by similar staining of these cells in allograft organs by a monoclonal antibody to nerve growth factor receptor, previously shown to recognize DC. DCs were generally not seen in normal control organs or portions of allografts uninvolved by lymphoid aggregates. This study provides evidence that 1) endothelial cell expression of VCAM-1 may be important in transplant rejection, 2) different epitopes of VCAM-1 may be preserved in tissue sections and recognized by different antibodies, and 3) there is probably a population of VCAM-1 expressing DC that participates in the cellular rejection progress.

[1]  R. Ross,et al.  Vascular cell adhesion molecule-1 is induced on endothelium during acute rejection in human cardiac allografts. , 1992, The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation.

[2]  E. Clark,et al.  Cultured human follicular dendritic cells. Growth characteristics and interactions with B lymphocytes. , 1992, Journal of immunology.

[3]  C. Orosz,et al.  Role of the endothelial adhesion molecule VCAM in murine cardiac allograft rejection. , 1992, Immunology letters.

[4]  M. Isobe,et al.  Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1. , 1992, Science.

[5]  J. J. van den Oord,et al.  Vascular adhesion molecules in acute and chronic liver inflammation , 1992, Hepatology.

[6]  C. Meijer,et al.  The VLA-4/VCAM-1 pathway is involved in lymphocyte adhesion to endothelium in rheumatoid synovium. , 1991, Journal of immunology.

[7]  L. Burkly,et al.  Signaling by vascular cell adhesion molecule‐1 (VCAM‐1) through VLA‐4 promotes CD3‐dependent T cell proliferation , 1991, European journal of immunology.

[8]  H. Grey,et al.  Analysis of T cell stimulation by superantigen plus major histocompatibility complex class II molecules or by CD3 monoclonal antibody: costimulation by purified adhesion ligands VCAM-1, ICAM-1, but not ELAM-1 , 1991, The Journal of experimental medicine.

[9]  C. Benjamin,et al.  Expression and functional characterization of a soluble form of vascular cell adhesion molecule 1. , 1991, Biochemical and biophysical research communications.

[10]  D. Weisenburger,et al.  Nerve growth factor receptor expression on dendritic reticulum cells in follicular lymphoid proliferations. , 1991, Human pathology.

[11]  S. Hubscher,et al.  Histological findings in liver allograft rejection—new insights into the pathogenesis of hepatocellular damage in liver allografts , 1991, Histopathology.

[12]  M. Cybulsky,et al.  Alternative splicing of human VCAM-1 in activated vascular endothelium. , 1991, The American journal of pathology.

[13]  G. Rice,et al.  Induced expression of endothelial-leukocyte adhesion molecules in human cardiac allografts. , 1991, Transplantation.

[14]  G. Rice,et al.  Vascular and nonvascular expression of INCAM-110. A target for mononuclear leukocyte adhesion in normal and inflamed human tissues. , 1991, The American journal of pathology.

[15]  C. Benjamin,et al.  Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1. , 1991, Blood.

[16]  T. Starzl,et al.  Early events in liver allograft rejection. Delineation of sites of simultaneous intragraft and recipient lymphoid tissue sensitization. , 1991, The American journal of pathology.

[17]  W. Baumgartner,et al.  Accelerated arteriosclerosis in heart transplant recipients is associated with a T-lymphocyte-mediated endothelialitis. , 1990, The American journal of pathology.

[18]  Prof. Dr. med. Gustav Steinhoff,et al.  Induction of ICAM-1 on hepatocyte membranes during liver allograft rejection and infection. , 1990, Transplantation proceedings.

[19]  J. Turunen,et al.  Characterization of high endothelial-like properties of peritubular capillary endothelium during acute renal allograft rejection. , 1990, The American journal of pathology.

[20]  J. Ludwig,et al.  Histologic Diagnosis of Rejection by Using: Cystoscopically Directed Needle Biopsy Specimens from Dysfunctional Pancreatoduodenal Allografts with Exocrine Drainage into the Bladder , 1990, The American journal of surgical pathology.

[21]  T. Carlos,et al.  Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial cells. , 1990, Blood.

[22]  G. Rice,et al.  Adhesion of human B cells to germinal centers in vitro involves VLA-4 and INCAM-110. , 1990, Science.

[23]  R. Colvin,et al.  In vivo effects of monoclonal antibody to ICAM-1 (CD54) in nonhuman primates with renal allografts. , 1990, Journal of immunology.

[24]  J. V. van Dongen,et al.  Antibody L26 recognizes an intracellular epitope on the B-cell-associated CD20 antigen. , 1990, The American journal of pathology.

[25]  A. Gown,et al.  Immunophenotype of vascular rejection in renal transplants. , 1990, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc.

[26]  Rodger P. McEver,et al.  Rapid neutrophil adhesion to activated endothelium mediated by GMP-140 , 1990, Nature.

[27]  C. Alpers,et al.  Platelets mediate glomerular cell proliferation in immune complex nephritis induced by anti-mesangial cell antibodies in the rat. , 1990, The American journal of pathology.

[28]  P. Morris,et al.  Migration of dendritic leukocytes from cardiac allografts into host spleens. A novel pathway for initiation of rejection , 1990, The Journal of experimental medicine.

[29]  A. Demetris,et al.  Liver allograft rejection: an overview of morphologic findings. , 1990, The American journal of surgical pathology.

[30]  R. Tizard,et al.  Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes , 1989, Cell.

[31]  R. Warnke,et al.  Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. , 1989, The American journal of pathology.

[32]  G. Bishop,et al.  Expression of leucocyte and lymphocyte adhesion molecules in the human kidney. , 1989, Kidney international.

[33]  J. Neuberger,et al.  INTERCELLULAR ADHESION MOLECULE 1 ON LIVER ALLOGRAFTS DURING REJECTION , 1989, The Lancet.

[34]  A. Gown,et al.  A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. , 1989, American journal of clinical pathology.

[35]  K. Batts,et al.  Endotheliitis in hepatic allografts. , 1989, Mayo Clinic proceedings.

[36]  G. Pinkus,et al.  Leu-22: a preferential marker for T-lymphocytes in paraffin sections. Staining profile in T- and B-cell lymphomas, Hodgkin's disease, other lymphoproliferative disorders, myeloproliferative diseases, and various neoplastic processes. , 1989, American journal of clinical pathology.

[37]  Michael Loran Dustin,et al.  Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1 , 1989, Nature.

[38]  R. Cook,et al.  Cloning of GMP-140, a granule membrane protein of platelets and endothelium: Sequence similarity to proteins involved in cell adhesion and inflammation , 1989, Cell.

[39]  B. Seed,et al.  Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. , 1989, Science.

[40]  T. Starzl,et al.  Chronic liver allograft rejection and obliterative arteriopathy: possible pathogenic mechanisms. , 1989, Transplantation proceedings.

[41]  A. Krensky,et al.  Microvascular destruction in renal transplant rejection. , 1989, Transplantation.

[42]  M. Hart,et al.  An analysis of the determinants of hepatic allograft rejection using stepwise logistic regression. , 1989, Transplantation.

[43]  Michael Loran Dustin,et al.  Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families , 1988, Cell.

[44]  B. Seed,et al.  ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM , 1988, Nature.

[45]  R. Cotran,et al.  Identification of an inducible endothelial-leukocyte adhesion molecule. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[46]  A. Norton,et al.  Monoclonal antibody L26: an antibody that is reactive with normal and neoplastic B lymphocytes in routinely fixed and paraffin wax embedded tissues. , 1987, Journal of Clinical Pathology.

[47]  D. Sutherland,et al.  Pancreas transplantation. An immunohistologic and histopathologic examination of 100 grafts. , 1987, The American journal of pathology.

[48]  R. Warnke,et al.  Endothelial cell phenotypic diversity. In situ demonstration of immunologic and enzymatic heterogeneity that correlates with specific morphologic subtypes. , 1987, American journal of clinical pathology.

[49]  D. V. van Thiel,et al.  Pathology of hepatic transplantation: A review of 62 adult allograft recipients immunosuppressed with a cyclosporine/steroid regimen. , 1985, The American journal of pathology.

[50]  J. Najarian,et al.  Orthotopic Liver Transplantation: A Pathological Study of 63 Serial Liver Biopsies from 17 Patients with Special Reference to the Diagnostic Features and Natural History of Rejection , 1984, Hepatology.

[51]  B. Steiniger,et al.  PHENOTYPE AND HISTOLOGICAL DISTRIBUTION OF INTERSTITIAL DENDRITIC CELLS IN THE RAT PANCREAS, LIVER, HEART, AND KIDNEY , 1984, Transplantation.

[52]  A. Gown,et al.  Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues. , 1984, The American journal of pathology.

[53]  M. Baran.  Pancreas transplantation. , 1982, The Diabetes educator.

[54]  S. Hsu,et al.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[55]  T E Starzl,et al.  Liver transplantation. , 1967, Bulletin de la Societe internationale de chirurgie.

[56]  N. Damle,et al.  Vascular cell adhesion molecule 1 induces T-cell antigen receptor-dependent activation of CD 4 ' T lymphocytes ( costimulation ) , 2022 .