Biological shape characterization for automatic image recognition and diagnosis of protozoan parasites of the genus Eimeria

We describe an approach of automatic feature extraction for shape characterization of seven distinct species of Eimeria, a protozoan parasite of domestic fowl. We used digital images of oocysts, a round-shaped stage presenting inter-specific variability. Three groups of features were used: curvature characterization, size and symmetry, and internal structure quantification. Species discrimination was performed with a Bayesian classifier using Gaussian distribution. A database comprising 3891 micrographs was constructed and samples of each species were employed for the training process. The classifier presented an overall correct classification of 85.75%. Finally, we implemented a real-time diagnostic tool through a web interface, providing a remote diagnosis front-end.

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