In situ single cell observation by fluorescence resonance energy transfer reveals fast intra‐cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine

The investigation into the intracellular mechanisms for gene expression has acquired great impetus for the improvement of the transfection efficiency by a non‐viral gene delivery system.

[1]  A. Kabanov,et al.  Evaluation of polyplexes as gene transfer agents. , 2001, Journal of controlled release : official journal of the Controlled Release Society.

[2]  Kozo Nakamura,et al.  Evaluation by fluorescence resonance energy transfer of the stability of nonviral gene delivery vectors under physiological conditions. , 2002, Biomacromolecules.

[3]  K Mechtler,et al.  The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells , 1998, Gene Therapy.

[4]  A S Verkman,et al.  Size-dependent DNA Mobility in Cytoplasm and Nucleus* , 2000, The Journal of Biological Chemistry.

[5]  A. Mikos,et al.  Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle. , 1999, Journal of biomedical materials research.

[6]  D. Fischer,et al.  A Novel Non-Viral Vector for DNA Delivery Based on Low Molecular Weight, Branched Polyethylenimine: Effect of Molecular Weight on Transfection Efficiency and Cytotoxicity , 1999, Pharmaceutical Research.

[7]  S. Carotta,et al.  Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo , 2001, The journal of gene medicine.

[8]  E. Brambilla,et al.  An electron microscopy study into the mechanism of gene transfer with lipopolyamines. , 1996, Gene therapy.

[9]  G. Levi,et al.  Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system , 1998, Gene Therapy.

[10]  Y. Tan,et al.  Targeted gene delivery to pulmonary endothelium by anti-PECAM antibody. , 2000, American journal of physiology. Lung cellular and molecular physiology.

[11]  J. Behr,et al.  Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold. , 1996, Gene therapy.

[12]  A. Mikos,et al.  Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[13]  S. W. Kim,et al.  Development of biomaterials for gene therapy. , 2000, Molecular therapy : the journal of the American Society of Gene Therapy.

[14]  C. Benoist,et al.  A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: polyethylenimine. , 1996, Human gene therapy.

[15]  Kabanov,et al.  Taking polycation gene delivery systems from in vitro to in vivo. , 1999, Pharmaceutical science & technology today.

[16]  A. Boletta,et al.  Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs , 1999, Gene Therapy.

[17]  J. Behr,et al.  Ovarian carcinoma cells are effectively transfected by polyethylenimine (PEI) derivatives , 2000, Cancer Gene Therapy.

[18]  Ernst Wagner,et al.  Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation. , 2002, Molecular therapy : the journal of the American Society of Gene Therapy.

[19]  S. Ferrari,et al.  ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo , 1997, Gene Therapy.

[20]  K. Kataoka,et al.  Water-soluble polyion complex associates of DNA and poly(ethylene glycol)-poly(L-lysine) block copolymer. , 1997, Bioconjugate chemistry.

[21]  H. Paik,et al.  Ionization of Poly(ethylenimine) and Poly(allylamine) at Various pH′s , 1994 .

[22]  O. Danos,et al.  Polyethylenimine‐mediated gene delivery: a mechanistic study , 2001, The journal of gene medicine.

[23]  A. Akinc,et al.  Measuring the pH environment of DNA delivered using nonviral vectors: implications for lysosomal trafficking. , 2002, Biotechnology and bioengineering.

[24]  Stefaan C. De Smedt,et al.  Cationic Polymer Based Gene Delivery Systems , 2000, Pharmaceutical Research.

[25]  D. W. Pack,et al.  On the kinetics of polyplex endocytic trafficking: implications for gene delivery vector design. , 2002, Molecular therapy : the journal of the American Society of Gene Therapy.

[26]  M. Buchberger,et al.  Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery , 1997, Gene Therapy.