论文信息 - Comment on “Widespread RNA and DNA Sequence Differences in the Human Transcriptome”

Comment on “Widespread RNA and DNA Sequence Differences in the Human Transcriptome”

Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported widespread differences between the RNA and DNA sequences of the same human cells, including all 12 possible mismatch types. Before accepting such a fundamental claim, a deeper analysis of the sequencing data is required to discern true differences between RNA and DNA from potential artifacts.

[1] Lior Pachter,et al. Identification and correction of systematic error in high-throughput sequence data , 2011, BMC Bioinformatics.

[2] K. Nishikura. Functions and regulation of RNA editing by ADAR deaminases. , 2010, Annual review of biochemistry.

[3] Cole Trapnell,et al. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome , 2009, Genome Biology.

[4] G. Church,et al. Genome-Wide Identification of Human RNA Editing Sites by Parallel DNA Capturing and Sequencing , 2009, Science.

[5] Mingyao Li,et al. Widespread RNA and DNA Sequence Differences in the Human Transcriptome , 2011, Science.

[6] W. J. Kent,et al. BLAT--the BLAST-like alignment tool. , 2002, Genome research.

[7] M. Metzker. Sequencing technologies — the next generation , 2010, Nature Reviews Genetics.

[8] N. Davidson,et al. APOBEC‐1‐mediated RNA editing , 2010, Wiley interdisciplinary reviews. Systems biology and medicine.