Enhancement of sickle erythrocyte adherence to endothelium by autologous platelets

The increased adhesiveness of sickle erythrocytes (SS RBC) to endothelial cells has been confirmed in a static system utilizing fresh umbilical vein endothelium. Adherence of SS RBC to the endothelium was as great in the presence of calcium‐containing buffer as when incubated in plasma. SS RBC suspended in autologous platelet‐rich plasma adhered to a greater extent than when suspended in autologous platelet poor plasma. Prostacyclin, thromboxane B2, and an inhibitor of collagen‐ and epinephrine‐induced platelet aggregation (B13.177) did not affect SS RBC adherence to endothelium. Aspirin in concentration of 5 μg/ml slightly decreased SS RBC adherence to endothelium in the presence of platelats may play a significant role in the increased adhesiveness of SS RBC to endothelium. To the extent that increasede SS RBC adhesiveness contributes to the genesis of painful crises and to the extent platelets augment this adhesiveness, agents affecting platelet function may prove useful in preventing painful crises.

[1]  J. L. Goodman,et al.  Potentiated adherence of sickle erythrocytes to endothelium infected by virus. , 1987, The Journal of clinical investigation.

[2]  J. Moake,et al.  Unusually large von Willebrand factor multimers increase adhesion of sickle erythrocytes to human endothelial cells under controlled flow. , 1987, The Journal of clinical investigation.

[3]  J. White,et al.  Pluronic F-68 reduces the endothelial adherence and improves the rheology of liganded sickle erythrocytes. , 1987, Blood.

[4]  E. Evans,et al.  Sickle erythrocyte adherence to vascular endothelium. Morphologic correlates and the requirement for divalent cations and collagen-binding plasma proteins. , 1985, The Journal of clinical investigation.

[5]  Nagel Rl,et al.  Adherence properties of sickle erythrocytes in dynamic flow systems. , 1985 .

[6]  J. Rosa,et al.  Clinical manifestations and erythrocyte adhesion to endothelium in sickle cell syndrome , 1985, American journal of hematology.

[7]  R. Aster,et al.  Use of prostacyclin to inhibit activation of platelets during preparation of platelet concentrates , 1984, Transfusion.

[8]  O. Ylikorkala,et al.  One milligramme of acetylsalicylic acid daily inhibits platelet thromboxane A2 production. , 1984, Thrombosis research.

[9]  E. Evans,et al.  Adherence of sickle erythrocytes to vascular endothelial cells: requirement for both cell membrane changes and plasma factors. , 1984, Blood.

[10]  J. Barbenel,et al.  Effect of prostacyclin (epoprostenol) on the aggregation of human platelets in whole blood in vitro. , 1984, Haemostasis.

[11]  C. Diglio,et al.  Diminished adherence of sickle erythrocytes to cultured vascular endothelium by piracetam , 1983, American journal of hematology.

[12]  M. Steinberg,et al.  Modulation of erythrocyte-endothelial interactions and the vasocclusive severity of sickling disorders. , 1981, Blood.

[13]  O. Ylikorkala,et al.  Measurement of thromboxane B2 in human plasma or serum by radioimmunoassay. , 1980, Prostaglandins.

[14]  J. Eaton,et al.  Erythrocyte adherence to endothelium in sickle-cell anemia. A possible determinant of disease severity. , 1980, The New England journal of medicine.

[15]  J. White,et al.  Abnormal adherence of sickle erythrocytes to cultured vascular endothelium: possible mechanism for microvascular occlusion in sickle cell disease. , 1980, The Journal of clinical investigation.

[16]  G. Wise,et al.  Adhesion of normal and sickle erythrocytes to endothelial monolayer cultures. , 1979, Blood.

[17]  M. Steinberg,et al.  Sickle cell anemia: Erythrokinetics, blood volumes, and a study of possible determinants of severity , 1977, American journal of hematology.

[18]  M. Karnovsky,et al.  Irreversible deformation of the spectrin-actin lattice in irreversibly sickled cells. , 1976, The Journal of clinical investigation.