Standing Surface Acoustic Wave Based Cell Coculture

Precise reconstruction of heterotypic cell–cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell–cell interactions.

[1]  Shuichi Takayama,et al.  Microfluidic hydrodynamic cellular patterning for systematic formation of co-culture spheroids. , 2009, Integrative biology : quantitative biosciences from nano to macro.

[2]  M. S. Steinberg,et al.  The differential adhesion hypothesis: a direct evaluation. , 2005, Developmental biology.

[3]  Tony Jun Huang,et al.  An on-chip, multichannel droplet sorter using standing surface acoustic waves. , 2013, Analytical chemistry.

[4]  Tze-Wen Chung,et al.  Evaluation of transdifferentiation from mesenchymal stem cells to neuron-like cells using microfluidic patterned co-culture system , 2011, Biomedical microdevices.

[5]  Robert L Sah,et al.  Probing the role of multicellular organization in three-dimensional microenvironments , 2006, Nature Methods.

[6]  M L Yarmush,et al.  Controlling cell interactions by micropatterning in co-cultures: hepatocytes and 3T3 fibroblasts. , 1997, Journal of biomedical materials research.

[7]  Sanghyo Kim,et al.  Microfluidic cell coculture methods for understanding cell biology, analyzing bio/pharmaceuticals, and developing tissue constructs. , 2011, Analytical biochemistry.

[8]  Cheng-Hsien Liu,et al.  Liver-cell patterning lab chip: mimicking the morphology of liver lobule tissue. , 2013, Lab on a chip.

[9]  T. Huang,et al.  Accelerating drug discovery via organs-on-chips. , 2013, Lab on a chip.

[10]  James J. Yoo,et al.  Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology. , 2013, Biomaterials.

[11]  Charless C. Fowlkes,et al.  Shrink‐Film Configurable Multiscale Wrinkles for Functional Alignment of Human Embryonic Stem Cells and their Cardiac Derivatives , 2011, Advanced materials.

[12]  R. Misra,et al.  Biomaterials , 2008 .

[13]  Tony Jun Huang,et al.  Surface acoustic wave (SAW) acoustophoresis: now and beyond. , 2012, Lab on a chip.

[14]  I-Kao Chiang,et al.  On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves , 2012, Proceedings of the National Academy of Sciences.

[15]  Peng Li,et al.  Surface acoustic wave microfluidics. , 2013, Lab on a chip.

[16]  I-Kao Chiang,et al.  Three-dimensional continuous particle focusing in a microfluidic channel via standing surface acoustic waves (SSAW). , 2011, Lab on a chip.

[17]  D. Grainger,et al.  A critical evaluation of in vitro cell culture models for high-throughput drug screening and toxicity. , 2012, Pharmacology & therapeutics.

[18]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[19]  G. Whitesides,et al.  Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Sangeeta N Bhatia,et al.  Micromechanical control of cell–cell interactions , 2007, Proceedings of the National Academy of Sciences.

[21]  Po-Hsun Huang,et al.  Tunable nanowire patterning using standing surface acoustic waves. , 2013, ACS nano.

[22]  Jianping Fu,et al.  Mechanobiology: a new frontier for human pluripotent stem cells. , 2013, Integrative biology : quantitative biosciences from nano to macro.

[23]  Fei Yan,et al.  Transportation of single cell and microbubbles by phase-shift introduced to standing leaky surface acoustic waves. , 2011, Biomicrofluidics.

[24]  Chung Yu Chan,et al.  Probing cell-cell communication with microfluidic devices. , 2013, Lab on a chip.

[25]  Guoping Chen,et al.  Heterotypic cell interactions on a dually patterned surface. , 2006, Biochemical and biophysical research communications.

[26]  Ali Khademhosseini,et al.  Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates. , 2006, Biomaterials.

[27]  Yuhong Pang,et al.  Quantitative study of the dynamic tumor-endothelial cell interactions through an integrated microfluidic coculture system. , 2012, Analytical chemistry.

[28]  Gwo-Bin Lee,et al.  Microfluidic cell culture systems for drug research. , 2010, Lab on a chip.

[29]  Shuichi Takayama,et al.  Polymeric Aqueous Biphasic Systems for Non‐Contact Cell Printing on Cells: Engineering Heterocellular Embryonic Stem Cell Niches , 2010, Advanced materials.

[30]  Deyu Li,et al.  A versatile valve-enabled microfluidic cell co-culture platform and demonstration of its applications to neurobiology and cancer biology , 2011, Biomedical microdevices.

[31]  Jianping Fu,et al.  Global architecture of the F-actin cytoskeleton regulates cell shape-dependent endothelial mechanotransduction. , 2014, Integrative biology : quantitative biosciences from nano to macro.

[32]  Claudia Tanja Mierke,et al.  Cancer Cells Regulate Biomechanical Properties of Human Microvascular Endothelial Cells* , 2011, The Journal of Biological Chemistry.

[33]  Burak Dura,et al.  Deformability-based microfluidic cell pairing and fusion. , 2014, Lab on a chip.

[34]  Ali Khademhosseini,et al.  Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components. , 2006, Biomaterials.

[35]  T. Huang,et al.  Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW). , 2009, Lab on a chip.

[36]  Luke P. Lee,et al.  Single-cell level co-culture platform for intercellular communication. , 2012, Integrative biology : quantitative biosciences from nano to macro.

[37]  Christopher K Zarins,et al.  A matrix micropatterning platform for cell localization and stem cell fate determination. , 2010, Acta biomaterialia.

[38]  Daniel Ahmed,et al.  Acoustic tweezers: patterning cells and microparticles using standing surface acoustic waves (SSAW). , 2009, Lab on a chip.

[39]  Robert T Kennedy,et al.  Continuous-flow enzyme assay on a microfluidic chip for monitoring glycerol secretion from cultured adipocytes. , 2009, Analytical chemistry.

[40]  Joe Tien,et al.  Fabrication of aligned microstructures with a single elastomeric stamp , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[41]  Aaron R Wheeler,et al.  A microfluidic platform for complete mammalian cell culture. , 2010, Lab on a chip.

[42]  A. Manz,et al.  Revisiting lab-on-a-chip technology for drug discovery , 2012, Nature Reviews Drug Discovery.

[43]  Wenfeng Liang,et al.  Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics. , 2014, Lab on a chip.

[44]  Jinjie Shi,et al.  Tunable patterning of microparticles and cells using standing surface acoustic waves. , 2012, Lab on a chip.

[45]  Ali Khademhosseini,et al.  Generation of static and dynamic patterned co-cultures using microfabricated parylene-C stencils. , 2007, Lab on a chip.

[46]  H Suzuki,et al.  Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices. , 2010, Acta biomaterialia.

[47]  Andreas Manz,et al.  Latest developments in microfluidic cell biology and analysis systems. , 2010, Analytical chemistry.

[48]  Charless C. Fowlkes,et al.  Integrated platform for functional monitoring of biomimetic heart sheets derived from human pluripotent stem cells. , 2014, Biomaterials.

[49]  O. MacDougald,et al.  Reversibly sealed multilayer microfluidic device for integrated cell perfusion and on-line chemical analysis of cultured adipocyte secretions , 2010, Analytical and bioanalytical chemistry.

[50]  K. Yosioka,et al.  Acoustic radiation pressure on a compressible sphere , 1955 .

[51]  Robert Langer,et al.  Engineering systems for the generation of patterned co-cultures for controlling cell-cell interactions. , 2011, Biochimica et biophysica acta.

[52]  J. Nör,et al.  Cross talk initiated by endothelial cells enhances migration and inhibits anoikis of squamous cell carcinoma cells through STAT3/Akt/ERK signaling. , 2009, Neoplasia.

[53]  Masayuki Yamato,et al.  Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture. , 2002, Biomaterials.

[54]  Shuichi Takayama,et al.  Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids. , 2009, Biomaterials.

[55]  Malcolm S. Steinberg,et al.  Reconstruction of Tissues by Dissociated Cells , 1963 .

[56]  Luke P. Lee,et al.  Robust pluripotent stem cell expansion and cardiomyocyte differentiation via geometric patterning. , 2013, Integrative biology : quantitative biosciences from nano to macro.

[57]  Nan Ma,et al.  Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration. , 2011, Biomaterials.

[58]  Ali Khademhosseini,et al.  Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures. , 2004, Biomaterials.

[59]  Andre Levchenko,et al.  Analysis of pairwise cell interactions using an integrated dielectrophoretic-microfluidic system , 2008, Molecular systems biology.

[60]  Aaron R Wheeler,et al.  A 3D microfluidic platform incorporating methacrylated gelatin hydrogels to study physiological cardiovascular cell-cell interactions. , 2013, Lab on a chip.

[61]  M L Yarmush,et al.  Effect of cell–cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells , 1999, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[62]  Yu Wang,et al.  Manipulating particle trajectories with phase-control in surface acoustic wave microfluidics. , 2011, Biomicrofluidics.

[63]  Laurent Griscom,et al.  In situ micropatterning technique by cell crushing for co-cultures inside microfluidic biochips , 2008, Biomedical microdevices.

[64]  R. Jaenisch,et al.  Microfluidic Control of Cell Pairing and Fusion , 2009, Nature Methods.

[65]  Daniel Ahmed,et al.  Focusing microparticles in a microfluidic channel with standing surface acoustic waves (SSAW). , 2008, Lab on a chip.

[66]  Matsuhiko Nishizawa,et al.  Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates. , 2009, Lab on a chip.