Pluronic polymersomes stabilized by core cross-linked polymer micelles

Vesicles from Pluronic L121 (PEO5-PPO68-PEO5) triblock copolymers were stabilized against aggregation by Pluronic P85 (PEO26-PPO40-PEO26) micelles. Both the vesicles and the micelles were reinforced by an interpenetrating polymer network from pentaerythritol tetraacrylate (PETA). AFM, electron microscopy and fluorescence labeling studies are used to investigate the morphology of the capsules. The P85 micelles are integrated into the L121 polymersome walls, while both types of Pluronic block copolymer are also non-covalently trapped in their respective interpenetrating acrylate networks. The micelle-containing vesicles retain their size upon heating until at least 33 °C. Upon cooling below room temperature the vesicles reversibly lose block copolymers. It was shown that the double-stabilized vesicles are readily internalized in HeLa and Caco-2 cells.

[1]  Jiayin Yuan,et al.  Wormlike morphology formation and stabilization of "pluronic p123" micelles by solubilization of pentaerythritol tetraacrylate. , 2008, Journal of Physical Chemistry B.

[2]  Qiang He,et al.  Transition of cationic dipeptide nanotubes into vesicles and oligonucleotide delivery. , 2007, Angewandte Chemie.

[3]  G. Tew,et al.  Self-assembled vesicles from an amphiphilic ortho-phenylene ethynylene macrocycle. , 2006, Angewandte Chemie.

[4]  A. Müller,et al.  Stabilization of polymeric micelles with a mixed poly(ethylene oxide)/poly(2-hydroxyethyl methacrylate) shell by formation of poly(pentaerythritol tetraacrylate) nanonetworks within the micelles , 2006 .

[5]  Britt A. Minch,et al.  Soft vesicles formed by diblock codendrimers of poly(benzyl ether) and poly(methallyl dichloride). , 2005, Journal of the American Chemical Society.

[6]  Dennis E Discher,et al.  Polymeric worm micelles as nano-carriers for drug delivery , 2005, Nanotechnology.

[7]  P. Petrov,et al.  Innovative approach for stabilizing poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) micelles by forming nano-sized networks in the micelle , 2005 .

[8]  A. Bergmann,et al.  Small-angle X-ray scattering, light scattering, and NMR study of PEO-PPO-PEO triblock copolymer/cationic surfactant complexes in aqueous solution. , 2005, The journal of physical chemistry. B.

[9]  L. J. P. van den Broeke,et al.  Reversible binding of multivalent ions by surfactant self-assembly. , 2005, Journal of the American Chemical Society.

[10]  D. Topgaard,et al.  Spontaneous Vesicle Formation in a Block Copolymer System , 2004 .

[11]  D. Discher,et al.  Membrane solubilization by detergent: resistance conferred by thickness. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[12]  D. Maysinger,et al.  Micellar Nanocontainers Distribute to Defined Cytoplasmic Organelles , 2003, Science.

[13]  F. Szoka,et al.  Synthesis of vesicles on polymer template. , 2002, Journal of the American Chemical Society.

[14]  A. Kabanov,et al.  Pluronic block copolymers: novel functional molecules for gene therapy. , 2002, Advanced drug delivery reviews.

[15]  Markus Antonietti,et al.  The formation of polymer vesicles or "peptosomes" by polybutadiene-block-poly(L-glutamate)s in dilute aqueous solution. , 2002, Journal of the American Chemical Society.

[16]  H. Kawaguchi,et al.  Functional polymer microspheres , 2000 .

[17]  K. Bryskhe,et al.  Vesicles Formed from a Poly(ethylene oxide)−Poly(propylene oxide)−Poly(ethylene oxide) Triblock Copolymer in Dilute Aqueous Solution , 1999 .

[18]  D. Hammer,et al.  Polymersomes: tough vesicles made from diblock copolymers. , 1999, Science.

[19]  M. Krishna.  Excited-State Kinetics of the Hydrophobic Probe Nile Red in Membranes and Micelles , 1999 .

[20]  Karin Schillén,et al.  Micellar Sphere-to-Rod Transition in an Aqueous Triblock Copolymer System. A Dynamic Light Scattering Study of a Translational and Rotational Diffusion , 1994 .

[21]  T. A. Hatton,et al.  Micellization of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymers in aqueous solutions: Thermodynamics of copolymer association , 1994 .

[22]  M. C. Stuart,et al.  Stabilization of Polymersome Vesicles by an Interpenetrating Polymer Network , 2007 .

[23]  Daniel T Kamei,et al.  Polyarginine segments in block copolypeptides drive both vesicular assembly and intracellular delivery. , 2007, Nature materials.

[24]  Dennis E. Discher,et al.  Polymer vesicles : Materials science: Soft surfaces , 2002 .