Micro-analysis of Plaque Fluid from Single-site Fasted Plaque

Despite the site-specific nature of caries, nearly all data on the concentration of ions relevant to the level of saturation of plaque fluid with respect to calcium phosphate minerals or enamel are from studies that used pooled samples. A procedure is described for the collection and analysis of inorganic ions relevant to these saturation levels in plaque fluid samples collected from a single surface on a single tooth. Various methods for examining data obtained by this procedure are described, and a mathematical procedure employing potential plots is recommended.

[1]  J. Ekstrand,et al.  Fluoride Analysis in Nanoliter- and Microliter-size Fluid Samples , 1990, Journal of dental research.

[2]  H. Margolis,et al.  Composition of Pooled Resting Plaque Fluid from Caries-free and Caries-susceptible Individuals , 1988, Journal of dental research.

[3]  H. Margolis,et al.  Composition of Human Plaque Fluid , 1988, Journal of dental research.

[4]  W. E. Brown,et al.  Micro-analysis of Mineral Saturation Within Enamel During Lactic Acid Demineralization , 1988, Journal of dental research.

[5]  T. M. Gregory,et al.  The buffer capacity of single-site, resting, human dental-plaque fluid. , 1988, Archives of oral biology.

[6]  L. Chow,et al.  Extracellular potassium concentrations in human dental plaque fluid recovered from single sites. , 1988, Archives of oral biology.

[7]  W. E. Brown,et al.  Micro-electrode Techniques for the Analysis of Oral Fluids , 1987, Journal of dental research.

[8]  G. Vogel,et al.  Re: The rapid determination of extracellular potassium concentrations in whole human dental plaque and plaque fluid. , 1987, Journal of dental research.

[9]  W. E. Brown,et al.  Ultramicro analysis of the fluid in human enamel during in vitro caries attack by hydrochloric acid. , 1987, Caries research.

[10]  W. E. Brown,et al.  Effects of Neutral Salts in a Bench-scale Caries Model , 1986, Journal of dental research.

[11]  C. Carey The driving forces in human dental plaque fluid for demineralisation and remineralisation of enamel mineral , 1986 .

[12]  H. Margolis,et al.  Micro-analytical Determination of pH, Calcium, and Phosphate in Plaque Fluid , 1985, Journal of dental research.

[13]  W. E. Brown,et al.  A Physicochemical Bench-scale Caries Model , 1984, Journal of dental research.

[14]  J. Noebels Ion-Sensitive Intracellular Microelectrodes. How to Make and Use Them , 1979, The Yale Journal of Biology and Medicine.

[15]  J. van Houte,et al.  Relationship of Streptococcus mutans and lactobacilli to incipient smooth surface dental caries in man. , 1978, Archives of oral biology.

[16]  A. Tatevossian,et al.  The composition of the aqueous phase in human dental plaque. , 1976, Archives of oral biology.

[17]  W. E. Brown,et al.  Thermodynamic Solubility Product of Human Tooth Enamel: Powdered Sample , 1975, Journal of dental research.

[18]  W. E. Brown,et al.  Formation of CaHPO4 · 2H2O from Enamel Mineral and Its Relationship to Caries Mechanism , 1975, Journal of dental research.

[19]  W. E. Brown Physicochemical Mechanisms of Dental Caries , 1974, Journal of dental research.

[20]  W. E. Brown,et al.  Solubility study of calcium hydrogen phosphate. Ion-pair formation , 1971 .

[21]  D. Howell,et al.  Demonstration of macromolecular inhibitors of calcification and nucleational factors in fluid from calcifying sites in cartilage. , 1969, The Journal of clinical investigation.

[22]  Walter E. Brown,et al.  Preparation and Solubility of Hydroxyapatite. , 1968, Journal of research of the National Bureau of Standards. Section A, Physics and chemistry.

[23]  P. Grøn,et al.  Human dental calculus. Inorganic chemical and crystallographic composition. , 1967, Archives of oral biology.

[24]  J. Macgregor Blood: : bone equilibrium in calcium homeostasis, progress report , 1964 .