论文信息 - Cryo-FIB Lift-out Sample Preparation Using a Novel Cryo-gripper Tool

Cryo-FIB Lift-out Sample Preparation Using a Novel Cryo-gripper Tool

In recent years, biological specimen preparation using a cryo-focused ion beam (cryo-FIB) has become a key technique for investigating complex cellular structures in situ [1-3]. Cryo-FIB has enabled cryoelectron tomography (cryo-ET) of plunge-frozen hydrated cells, revealing the cellular interior with sufficient resolution and contrast to study membrane-bound macromolecules in their native state [4-7].

[1] Friedrich Förster,et al. Dissecting the molecular organization of the translocon-associated protein complex , 2017, Nature Communications.

[2] W. Baumeister,et al. Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins. , 2017, Journal of structural biology.

[3] W. Baumeister,et al. Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography. , 2015, Bio-protocol.

[4] Wolfgang Baumeister,et al. In situ structural analysis of Golgi intracisternal protein arrays , 2015, Proceedings of the National Academy of Sciences.

[5] W. Baumeister,et al. In Situ Tomography of Membrane Proteins Enabled by Advanced Cryo-FIB Sample Preparation and Phase Plate Imaging , 2015, Microscopy and Microanalysis.

[6] W. Baumeister,et al. Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography , 2015, eLife.

[7] R. Schalek,et al. Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy , 2007, Nature Methods.