Studies on Milk-clotting Enzyme of Dothiorella ribis -Part I. The Production of Milk-clotting Enzyme-

Microorganisms producing milk-clotting enzyme were isolated from 1,506 strains which were collected from soil on the various places of Korea, and from strains which were already identified. Dothiorella ribis was taken as a good strain producing milk-clotting enzyme. When it is cultured on wheat bran, the optimum experimental conditions for the production of milk-clotting enzyme were consequently obtained as follows: 1) of temperature and 4.0 of pH. 2) of cultivating water to the weight of wheat bran. 3) addition of as a nitrogen source, as an inorganic salt, and 3% of sucrose as a carbon source. 4) four days for a period of cultivation.