Effect of formalin fixation on thermal conductivity of the biological tissue.

Effect of formalin fixation on thermal conductivity of the biological tissues is presented. A self-heated thermistor probe was used to measure the tissue thermal conductivity. The thermal conductivity of muscle and fatty tissue samples was measured before the formalin fixation and then 27 hours after formalin fixation. The results indicate that the formalin fixation does not cause a significant change in the tissue thermal conductivity of muscle and fatty tissues. In the clinical setting, tissues removed surgically are often fixed in formalin for subsequent pathological analysis. These results suggest that, in terms of thermal properties, it is equally appropriate to perform in vitro studies in either fresh tissue or formalin-fixed tissue.

[1]  J. W. Valvano,et al.  Thermal conductivity and diffusivity of arterial wall and atherosclerotic plaque , 1987 .

[2]  K. Helander,et al.  Kinetic studies of formaldehyde binding in tissue. , 1994, Biotechnic & histochemistry : official publication of the Biological Stain Commission.

[3]  K R Holmes,et al.  Pulse-decay method for measuring the thermal conductivity of living tissues. , 1981, Journal of biomechanical engineering.

[4]  Jing Liu,et al.  Sinusoidal heating method to noninvasively measure tissue perfusion , 2002, IEEE Transactions on Biomedical Engineering.

[5]  J A Pearce,et al.  A self-heated thermistor technique to measure effective thermal properties from the tissue surface. , 1987, Journal of biomechanical engineering.

[6]  J Dwyer,et al.  Fixation protocols for subcellular imaging by synchrotron‐based Fourier transform infrared microspectroscopy , 2005, Biopolymers.

[7]  J.W. Valvano,et al.  Self-heated thermistor measurements of perfusion , 1992, IEEE Transactions on Biomedical Engineering.

[8]  L. J. Hayes,et al.  Pulse-power integrated-decay technique for the measurement of thermal conductivity , 2008 .

[9]  H.Frederick Bowman,et al.  A new technique utilizing thermistor probes for the measurement of thermal properties of biomaterials. , 1976, Cryobiology.

[10]  Jonathan W. Valvano,et al.  Thermal conductivity and diffusivity of biomaterials measured with self-heated thermistors , 1985 .

[11]  R. Scott.  Plasma membrane vesiculation: a new technique for isolation of plasma membranes. , 1976, Science.

[12]  A. Chott,et al.  Effect of formalin tissue fixation and processing on immunohistochemistry. , 2000, The American journal of surgical pathology.

[13]  Francis A. Duck,et al.  Physical properties of tissue : a comprehensive reference book , 1990 .

[14]  S. Jewell,et al.  Copyright © American Society for Investigative Pathology Review Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids , 2022 .

[15]  T. O'Leary,et al.  Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation. , 1991, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[16]  D. French,et al.  The Reactions of Formaldehyde with Amino Acids and Proteins , 1945 .

[17]  S. Cross,et al.  Reassessment of the rate of fixative diffusion. , 1992, Journal of clinical pathology.

[18]  F. Duck.  Physical properties of tissue , 1990 .

[19]  Peter Libby,et al.  Atherosclerosis: disease biology affecting the coronary vasculature. , 2006, The American journal of cardiology.

[20]  G. Gresham,et al.  Reaction of Formaldehyde with Unsaturated Fatty Acids during Histological Fixation , 1966, Nature.

[21]  P. Roller,et al.  Formaldehyde fixation. , 1985, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[22]  D. Hopwood,et al.  Fixatives and fixation: a review , 1969, The Histochemical Journal.