Objectives: To define the profile of 24‐hour uterine activity in normal pregnancy and to correlate contraction frequency with physical activity and emotional stress diaries. Methods: One hundred nine low‐risk pregnant women who delivered at term recorded uterine contractions for 24 hours twice weekly from 20‐40 weeks' gestation using an ambulatory monitor and kept a physical activity and emotional stress diary. Contractions per hour were calculated for each hour of the day and week of gestation, and related to physical activity and emotional stress. Results: We analyzed 71,683 hours. No contractions were recorded in 73% of the hours, and fewer than four contractions per hour occurred in 96%. Significant inter‐individual variability was noted. Contractions increased markedly with gestational age: The 95th percentile was 1.3 contractions per hour at 21‐24 weeks, 2.9 at 28‐32 weeks, and 4.9 at 38‐40 weeks. A strong clustering of contractions occurred at night, which became pronounced after 24 weeks (night:day ratio 2:1 at 28‐32 weeks). To adjust for the effects of gestational age and time of day, contractions per hour were converted to gestation‐ and hour‐specific percentiles (“contraction percentiles”). Rest was associated with a fall in contraction percentile by 1.25, whereas coitus increased the contraction percentile by 5.52 (P < .05). No changes were noted with emotional stress. Conclusions: This study provides normative contraction data in uncomplicated pregnancy. A strong diurnal rhythm is present from 24 weeks onward, with 67% of contractions occurring at night. Contractions per hour increase with gestational age but rarely exceed three per hour before term. Rest and sexual activity have small but measurable effects on contraction frequency. (Obstet Gynecol 1994;83:517‐23)