Strawberries rotted by pink mold rot fungus (Trichothecium roseum) were found sporadically in farm greenhouses in Sugokmyon, Jinju, Korea, from March to April in 2009 and 2010. The diseased fruits were first covered with pink-colored mold, consisting of conidia and conidiophores of the pathogen, and then became water-soaked and dark brown in color, before eventually rotting (Fig. 1A). After a pure representative specimen was isolated, we examined the mycological characteristics of the specimens in detail, using light and scanning electron microscopes. Fungal colonies were white to pinkish, and numerous conidia formed on potato dextrose agar (PDA; Fig. 1C). Conidia were ellipsoidal to pyriform, colorless, 2-celled, characteristically held together in zigzag chains, aleurioconidia 12-21×8-10 μm in size (Fig. 1D). Conidiophores were long, slender, simple, septate, bearing meristem arthrospore conidia apically, singly when young, and successively. Conidiophores were apex, 3-5 μm in size. The optimum temperature for mycelial growth was 25 oC. The measurements and taxonomic characteristics coincided with those of T. roseum (Pers.) Link ex Gray described by Gobayashi et al. (1992) and Ishikawa et al. (1998). The pure isolate of the pathogen was incubated at 25 oC, and the conidia were artificially inoculated onto strawberries. The same disease symptoms were reproduced on the fruits, and the same fungus was re-isolated from the symptoms. To identify the causal fungus, we amplified and sequenced a partial internal transcribed space (ITS) rDNA of the isolate, using the primers ITS1 and ITS4, as described by White et al. (1990). The resulting sequence of 613 bp was deposited in GenBank (accession no. HM355750). Phylogenetic analysis was conduced using MEGA4 software, with the neighbor-joining method and TajimaNei distance model. The neighbor-joining analysis (Fig. 2) showed that the ITS sequences of the isolate were similar to those of T. roseum (Seifert et al., 1997). In the phylogenetic tree (Fig. 2), the isolate was placed within a clade comprising reference isolates of T. roseum. This is the first report of pink mold rot on strawberry caused by T. roseum in Korea. Cultures of T. roseum have been deposited with the Korean Agricultural Culture Collection (KACC 45158), National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon.