Assessing methods for blood cell cytotoxic responses to inorganic nanoparticles and nanoparticle aggregates.

Inorganic nanoparticles (NPs) show great potential for medicinal therapy. However, biocompatibility studies are essential to determine if they are safe. Here, five different NPs are compared for their cytotoxicity, internalization, aggregation in medium, and reactive oxygen species (ROS) production, using tumoral and normal human blood cells. Differences depending on the cell type are analyzed, and no direct correlation between ROS production and cell toxicity is found. Results are discussed with the aim of standardizing the procedures for the evaluation of the toxicity.

[1]  J. Santamaría,et al.  Synthesis and stealthing study of bare and PEGylated silica micro- and nanoparticles as potential drug-delivery vectors , 2008 .

[2]  C. Mikoryak,et al.  Single-walled carbon nanotube interactions with HeLa cells , 2007, Journal of nanobiotechnology.

[3]  Chung-Yuan Mou,et al.  The effect of surface charge on the uptake and biological function of mesoporous silica nanoparticles in 3T3-L1 cells and human mesenchymal stem cells. , 2007, Biomaterials.

[4]  Jordi Arbiol,et al.  Antibody‐Functionalized Hybrid Superparamagnetic Nanoparticles , 2007 .

[5]  J. Santamaría,et al.  Magnetic nanoparticles for drug delivery , 2007 .

[6]  Sung Ju Cho,et al.  Quantum dot-induced cell death involves Fas upregulation and lipid peroxidation in human neuroblastoma cells , 2007, Journal of nanobiotechnology.

[7]  Shao-Hua Yang,et al.  Pyruvate protects mitochondria from oxidative stress in human neuroblastoma SK-N-SH cells , 2007, Brain Research.

[8]  J. Santamaría,et al.  Sustained release of doxorubicin from zeolite–magnetite nanocomposites prepared by mechanical activation , 2006, Nanotechnology.

[9]  Robert H. Hurt,et al.  Toxicology of carbon nanomaterials: Status, trends, and perspectives on the special issue , 2006 .

[10]  H. Krug,et al.  Oops they did it again! Carbon nanotubes hoax scientists in viability assays. , 2006, Nano letters.

[11]  T. Xia,et al.  Toxic Potential of Materials at the Nanolevel , 2006, Science.

[12]  Aibing Yu,et al.  Inorganic nanoparticles as carriers for efficient cellular delivery , 2006 .

[13]  Eduarda Fernandes,et al.  Fluorescence probes used for detection of reactive oxygen species. , 2005, Journal of biochemical and biophysical methods.

[14]  J. West,et al.  Nano-C60 cytotoxicity is due to lipid peroxidation. , 2005, Biomaterials.

[15]  Simon C Watkins,et al.  Sulforaphane-induced Cell Death in Human Prostate Cancer Cells Is Initiated by Reactive Oxygen Species* , 2005, Journal of Biological Chemistry.

[16]  Weizhen Zeng,et al.  Organic modified mesoporous MCM-41 through solvothermal process as drug delivery system , 2005 .

[17]  C. Marquina,et al.  From magnetoelectronic to biomedical applications based on the nanoscale properties of advanced magnetic materials , 2005 .

[18]  M. Alonso,et al.  Nanoparticles as carriers for nasal vaccine delivery , 2005, Expert review of vaccines.

[19]  É. Duguet,et al.  Magnetic nanoparticle design for medical diagnosis and therapy , 2004 .

[20]  M. Karin,et al.  Essential Roles of Receptor-Interacting Protein and TRAF2 in Oxidative Stress-Induced Cell Death , 2004, Molecular and Cellular Biology.

[21]  G. Shi,et al.  The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species , 2004, Cell Research.

[22]  R. Stafford,et al.  Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[23]  G. Alberti,et al.  Determination of the total concentration and speciation of Al(III) in tea infusions. , 2003, Journal of inorganic biochemistry.

[24]  Raoul Kopelman,et al.  Room-temperature preparation and characterization of poly (ethylene glycol)-coated silica nanoparticles for biomedical applications. , 2003, Journal of biomedical materials research. Part A.

[25]  G. Tolnai,et al.  Preparation and characterization of surface-modified silica-nanoparticles , 2001 .

[26]  D. Housman,et al.  Functional genomics reveals a family of eukaryotic oxidation protection genes. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[27]  R. Tiozzo,et al.  Pure-silica zeolites (Porosils) as model solids for the evaluation of the physicochemical features determining silica toxicity to macrophages. , 2000, Chemical research in toxicology.

[28]  D. Zhao,et al.  Fabrication of Ordered Porous Structures by Self-Assembly of Zeolite Nanocrystals , 2000 .

[29]  W. Krätschmer,et al.  Solid C60: a new form of carbon , 1990, Nature.

[30]  P. Cresswell,et al.  NK susceptibility varies inversely with target cell class I HLA antigen expression. , 1987, Journal of immunology.

[31]  W. Webb,et al.  Fluorescent erythrosin B is preferable to trypan blue as a vital exclusion dye for mammalian cells in monolayer culture. , 1984, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[32]  T. Osawa.  Human T cell hybridomas producing inflammatory lymphokines , 1984 .

[33]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[34]  J. Watson,et al.  Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line , 1980, The Journal of experimental medicine.

[35]  K. Nilsson,et al.  Establishment and characterization of a human histiocytic lymphoma cell line (U‐937) , 1976, International journal of cancer.

[36]  Jinwoo Cheon,et al.  Artificially engineered magnetic nanoparticles for ultra-sensitive molecular imaging , 2007, Nature Medicine.

[37]  David B Warheit,et al.  Pulmonary bioassay studies with nanoscale and fine-quartz particles in rats: toxicity is not dependent upon particle size but on surface characteristics. , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[38]  Scott C. Brown,et al.  Influence of shape, adhesion and simulated lung mechanics on amorphous silica nanoparticle toxicity , 2007 .

[39]  María J Vicent,et al.  Polymer conjugates: nanosized medicines for treating cancer. , 2006, Trends in biotechnology.

[40]  W. Wąsowicz,et al.  Roles of reactive oxygen species and selected antioxidants in regulation of cellular metabolism. , 2005, International journal of occupational medicine and environmental health.