Diol derivative of (3-trifluoromethyl)phenyldiazirine for post-labeling of photocrosslink

3-Trifluoromethylphenyldiazirinylated diol derivative was utilized to introduce an aldehyde by periodate oxidation, followed by the formation of a Schiff base with biotin hydrazide on a PVDF membrane for post-labeling of photocrosslinked proteins. The biotin hydrazide was able to post-label the 2.5 x 10 - 1 3 mole of closslinked component for chemiluminescent visualization.