ALIZARIN RED S AS AN INTRAVITAL FLUOROCHROME IN MINERALIZING TISSUES.

To avoid interference with weight gain following alizarin red S injections, low doses of an order of 25 mg/kg body weight must be used. Two such doses given 4 days apart to 24-day-old rats produced no visible staining of bone or dentine in undecalcified sections examined by ordinary light microscopy. Under ultraviolet light, however, staining was evident in the form of fine, red fluorescent lines.

[1]  D. Hoyte Alizarin as an indicator of bone growth. , 1960, Journal of anatomy.