Supercritical fluids extraction and anti-solvent purification of carotenoids from microalgae and associated bioactivity

Abstract This study investigated co-solvent modified supercritical carbon dioxide extraction of lipids and carotenoids from the microalgal species of Nannochloropsis oculata . Supercritical carbon dioxide (SCCO 2 ) anti-solvent precipitation of carotenoids from the extracts following purification of Zeaxanthin was also examined. Continuous modification by ethanol of supercritical carbon dioxide extractions showed that the addition ratio was important for extraction efficiency of lipids and carotenoids. SCCO 2 extraction at 350 bar, 323 K and 16.7 wt% of ethanol addition yielded 239.7 mg of triglycerides and 7.61 mg of carotenoids per gram extract with a total yield of 15.5%. SCCO 2 anti-solvent experiments showed that the content of Zeaxanthin in the precipitate was greater than that in the fraction of normal phase column chromatography. The purest Zeaxanthin (93.8%) was then successfully isolated from the purified fraction by using a reverse-phase HPLC column chromatography. Rat macrophages treated by ultra-sonicated water extracts of the SCCO 2 defatted algae showed a positive phagocytotic activity.

[1]  M. Freire-Garabal,et al.  Anti‐inflammatory and immunomodulatory activities of polysaccharide from Chlorella stigmatophora and Phaeodactylum tricornutum , 2003, Phytotherapy research : PTR.

[2]  M. J. Cocero,et al.  Supercritical anti solvent precipitation of lycopene: Effect of the operating parameters , 2006 .

[3]  S. Lai,et al.  Biodiesel production from supercritical carbon dioxide extracted Jatropha oil using subcritical hydrolysis and supercritical methylation , 2010 .

[4]  L. Rodolfi,et al.  Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor , 2009, Biotechnology and bioengineering.

[5]  S. Hsu,et al.  Co-solvent-modified supercritical carbon dioxide extractions of cholesterol and free amino acids from soft-shell turtle fish egg , 2008 .

[6]  S. Purton,et al.  Microalgae as bioreactors , 2005, Plant Cell Reports.

[7]  S. Lai,et al.  Recovery of vitamins B from supercritical carbon dioxide-defatted rice bran powder using ultrasound water extraction , 2011 .

[8]  E. Ibáñez,et al.  Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae: A review , 2006 .

[9]  S. Hsu,et al.  Precipitation of sub-micron particles of 3,5-diprenyl-4-hydroxycinnamic acid in Brazilian propolis from supercritical carbon dioxide anti-solvent solutions , 2009 .

[10]  P. V. Suresh,et al.  Recovery of carotenoids from ensilaged shrimp waste. , 2007, Bioresource technology.

[11]  M. J. Cocero,et al.  Co-precipitation of carotenoids and bio-polymers with the supercritical anti-solvent process , 2007 .

[12]  Feng Chen,et al.  Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography. , 2005, Journal of chromatography. A.

[13]  E. Martínez de la Ossa,et al.  Extraction of carotenoids and chlorophyll from microalgae with supercritical carbon dioxide and ethanol as cosolvent. , 2008, Journal of separation science.

[14]  P. Margalith.  Production of ketocarotenoids by microalgae , 1999, Applied Microbiology and Biotechnology.

[15]  P. Bhosale,et al.  Environmental and cultural stimulants in the production of carotenoids from microorganisms , 2003, Applied Microbiology and Biotechnology.

[16]  F. García-Camacho,et al.  Biomass nutrient profiles of the microalga Nannochloropsis. , 2001, Journal of agricultural and food chemistry.

[17]  A. Randolph,et al.  Precipitation of microsize organic particles from supercritical fluids , 1989 .