Unintended compositional changes in transgenic rice seeds ( Oryza sativa L.) studied by spectral and chromatographic analysis coupled with chemometrics methods.

Unintended compositional changes in transgenic rice seeds were studied by near-infrared reflectance, GC-MS, HPLC, and ICP-AES coupled with chemometrics strategies. Three kinds of transgenic rice with resistance to fungal diseases or insect pests were comparatively studied with the nontransgenic counterparts in terms of key nutrients such as protein, amino acids, fatty acids, vitamins, elements, and antinutrient phytic acid recommended by the Organization for Economic Co-operation and Development (OECD). The compositional profiles were discriminated by chemometrics methods, and the discriminatory compounds were protein, three amino acids, two fatty acids, two vitamins, and several elements. Significance of differences for these compounds was proved by analysis of variance, and the variation extent ranged from 20 to 74% for amino acids, from 19 to 38% for fatty acids, from 25 to 57% for vitamins, from 20 to 50% for elements, and 25% for protein, whereas phytic acid content did not change significantly. The unintended compositional alterations as well as unintended change of physical characteristic in transgenic rice compared with nontransgenic rice might be related to the genetic transformation, the effect of which needs to be elucidated by additional studies.

[1]  Daniel Cozzolino,et al.  Usefulness of near-infrared reflectance (NIR) spectroscopy and chemometrics to discriminate fishmeal batches made with different fish species. , 2005, Journal of agricultural and food chemistry.

[2]  P. Vasudevan,et al.  An overview of bacterial blight disease of rice and strategies for its management , 2000 .

[3]  Nigel W. Hardy,et al.  Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[4]  Y. Nishizawa,et al.  Characterization of transgenic rice plants over-expressing the stress-inducible β-glucanase gene Gns1 , 2004, Plant Molecular Biology.

[5]  Marcelo Blanco,et al.  NIR spectroscopy: a rapid-response analytical tool , 2002 .

[6]  D. Cozzolino,et al.  Feasibility study on the use of visible and near-infrared spectroscopy together with chemometrics to discriminate between commercial white wines of different varietal origins. , 2003, Journal of agricultural and food chemistry.

[7]  Maurizio Aceto,et al.  Statistical investigation of the differences in the distribution of metals in Nebbiolo-based wines , 2003 .

[8]  H. Li,et al.  Comparison of nutritional quality between Chinese indica rice with sck and cry1Ac genes and its nontransgenic counterpart. , 2007, Journal of food science.

[9]  S. Utsumi,et al.  Quality and safety evaluation of genetically engineered rice with soybean glycinin: analyses of the grain composition and digestibility of glycinin in transgenic rice. , 1999, Bioscience, biotechnology, and biochemistry.

[10]  J. Lehrfeld.  HPLC Separation and Quantitation of Phytic Acid and Some Inositol Phosphates in Foods: Problems and Solutions , 1994 .

[11]  M. Fountoulakis,et al.  Effect of the hydrolysis method on the determination of the amino acid composition of proteins. , 1998, Journal of chromatography. A.

[12]  Analysis of the conditional correlations from different genetic systems between the protein content and the appearance quality traits of indica rice. , 2007, Journal of genetics and genomics = Yi chuan xue bao.

[13]  I. Nishida,et al.  An increase in unsaturation of fatty acids in phosphatidylglycerol from leaves improves the rates of photosynthesis and growth at low temperatures in transgenic rice seedlings. , 2002, Plant & cell physiology.

[14]  C. Cimpoiu,et al.  Separation, identification and quantitative determination of free amino acids from plant extracts. , 1998, Journal of pharmaceutical and biomedical analysis.

[15]  B. P. Klein,et al.  Carotene, tocopherol, and ascorbate contents in subspecies of Brassica oleracea. , 1999, Journal of agricultural and food chemistry.

[16]  E. Hinchliffe,et al.  Improving the nutritional value of Golden Rice through increased pro-vitamin A content , 2005, Nature Biotechnology.

[17]  R. Shillito,et al.  Rice (Oryza sativa L.) containing the bar gene is compositionally equivalent to the nontransgenic counterpart. , 2005, Journal of agricultural and food chemistry.

[18]  C. Weaver,et al.  Mineral Bioavailability in Rats from Intrinsically Labeled Whole Wheat Flour of Various Phytate Levels , 1994 .

[19]  Zhu Zhen,et al.  Increasing Accumulation Level of Foreign Protein in Transgenic Plants Through Protein Targeting , 2003 .

[20]  L. Rooney,et al.  Perspectives on nixtamalization (alkaline cooking) of maize for tortillas and snacks , 1999 .

[21]  A. Savitzky,et al.  Smoothing and Differentiation of Data by Simplified Least Squares Procedures. , 1964 .

[22]  G. Le Gall,et al.  Metabolite profiling of tomato (Lycopersicon esculentum) using 1H NMR spectroscopy as a tool to detect potential unintended effects following a genetic modification. , 2003, Journal of agricultural and food chemistry.

[23]  Xiaping Fu,et al.  Discrimination of transgenic tomatoes based on visible/near-infrared spectra. , 2007, Analytica chimica acta.

[24]  P. Waterhouse,et al.  Ectopic expression of the spike protein of Rice Ragged Stunt Oryzavirus in transgenic rice plants inhibits transmission of the virus to insects , 2003, Molecular Breeding.

[25]  Liu Dong-hu.  Resistance of Transgenic Rice Containing Both sck and cry1Ac Genes Against Chilo suppressalis and Cnaphalocrocis medinalis , 2004 .

[26]  P. Finglas,et al.  Certification of B-group vitamins (B1, B2, B6, and B12) in four food reference materials. , 2001, Journal of agricultural and food chemistry.