HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5

The β-chemokines MIP-1α, MIP-1β and RANTES inhibit infection of CD4+ cells by primary, non-syncytium-inducing (NSI) HIV-1 strains at the virus entry stage, and also block env-mediated cell–cell membrane fusion. CD4+ T cells from some HIV-1-exposed uninfected individuals cannot fuse with NSI HIV-1 strains and secrete high levels of β-chemokines. Expression of the β-chemokine receptor CC-CKR-5 in CD4+ , non-permissive human and non-human cells renders them susceptible to infection by NSI strains, and allows env-mediated membrane fusion. CC-CKR-5 is a second receptor for NSI primary viruses.

[1]  J. Bentz Viral Fusion Mechanisms , 1993 .

[2]  B. Premack,et al.  Activation of dual T cell signaling pathways by the chemokine RANTES. , 1995, Science.

[3]  R. Blumenthal,et al.  Heat-resistant factors in human erythrocyte membranes mediate CD4-dependent fusion with cells expressing HIV-1 envelope glycoproteins. , 1996, Virology.

[4]  Paul E. Kennedy,et al.  HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein-Coupled Receptor , 1996, Science.

[5]  P. Charneau,et al.  Complementation of murine cells for human immunodeficiency virus envelope/CD4-mediated fusion in human/murine heterokaryons , 1992, Journal of virology.

[6]  P. Young,et al.  Monocyte Chemotactic Protein-3 (MCP3) Interacts with Multiple Leukocyte Receptors , 1995, The Journal of Biological Chemistry.

[7]  M. Peitsch,et al.  Selectivity and antagonism of chemokine receptors , 1996, Journal of leukocyte biology.

[8]  T. Schall,et al.  Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor , 1993, Cell.

[9]  S. Arya,et al.  Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells , 1995, Science.

[10]  L. Picard,et al.  Proteinase-resistant factors in human erythrocyte membranes mediate CD4-dependent fusion with cells expressing human immunodeficiency virus type 1 envelope glycoproteins , 1995, Journal of virology.

[11]  J. Levy,et al.  Controlling HIV pathogenesis: the role of the noncytotoxic anti-HIV response of CD8+ T cells. , 1996, Immunology today.

[12]  M. Reitz,et al.  Growth of macrophage-tropic and primary human immunodeficiency virus type 1 (HIV-1) isolates in a unique CD4+ T-cell clone (PM1): failure to downregulate CD4 and to interfere with cell-line-tropic HIV-1 , 1995, Journal of virology.

[13]  D. Ho,et al.  Human immunodeficiency virus type 1 variants with increased replicative capacity develop during the asymptomatic stage before disease progression , 1994, Journal of virology.

[14]  R. Horuk,et al.  Expression of the Duffy antigen in K562 cells. Evidence that it is the human erythrocyte chemokine receptor. , 1994, The Journal of biological chemistry.

[15]  R. Andino,et al.  Distinct modes of human immunodeficiency virus type 1 proviral latency revealed by superinfection of nonproductively infected cell lines with recombinant luciferase-encoding viruses , 1994, Journal of virology.

[16]  Steven M. Wolinsky,et al.  Relative resistance to HIV–1 infection of CD4 lymphocytes from persons who remain uninfected despite multiple high–risk sexual exposures , 1996, Nature Medicine.

[17]  U. Francke,et al.  Structure and functional expression of the human macrophage inflammatory protein 1 alpha/RANTES receptor , 1993, The Journal of experimental medicine.

[18]  B. Moss,et al.  Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells , 1990, Journal of virology.

[19]  R. Weiss,et al.  Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types 1 and 2 and by simian immunodeficiency virus , 1991, Virology.

[20]  G Vassart,et al.  Molecular cloning and functional expression of a new human CC-chemokine receptor gene. , 1996, Biochemistry.

[21]  L. F. Kolakowski,et al.  Functional and biochemical analysis of the cloned Duffy antigen: identity with the red blood cell chemokine receptor , 1994 .

[22]  R. Connor,et al.  Vpr is required for efficient replication of human immunodeficiency virus type-1 in mononuclear phagocytes. , 1995, Virology.

[23]  Robin A. Weiss,et al.  The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain , 1986, Cell.

[24]  R. Eddy,et al.  New members of the chemokine receptor gene family , 1996, Journal of leukocyte biology.

[25]  S. Coughlin,et al.  Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[26]  J. Van Damme,et al.  Monocyte Chemoattractant Protein-3 Is a Functional Ligand for CC Chemokine Receptors 1 and 2B (*) , 1995, The Journal of Biological Chemistry.

[27]  C. Combadière,et al.  Cloning and Functional Expression of a Human Eosinophil CC Chemokine Receptor (*) , 1995, The Journal of Biological Chemistry.

[28]  T. Geiser,et al.  Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. , 1994, The Journal of biological chemistry.