Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2–48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.

[1]  S. Chin,et al.  Integrated miniature fluorescent probe to leverage the sensing potential of ZnO quantum dots for the detection of copper (II) ions. , 2013, Talanta.

[2]  J. M. Leitão,et al.  Firefly luciferase inhibition. , 2010, Journal of photochemistry and photobiology. B, Biology.

[3]  E. Lakatta,et al.  A bioluminescence method for direct measurement of phosphodiesterase activity. , 2011, Analytical biochemistry.

[4]  Jie-Bi Hu,et al.  Automated system for extraction and instantaneous analysis of millimeter-sized samples , 2014 .

[5]  Jie-Bi Hu,et al.  A pinch-valve interface for automated sampling and monitoring of dynamic processes by gas chromatography-mass spectrometry , 2014 .

[6]  D. Endy.  Foundations for engineering biology , 2005, Nature.

[7]  Dan Ferber,et al.  Microbes Made to Order , 2004, Science.

[8]  C. Ribeiro,et al.  Kinetics of inhibition of firefly luciferase by oxyluciferin and dehydroluciferyl-adenylate , 2008, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[9]  Juan Enrique Agudo,et al.  A Compact and Low Cost Electronic Nose for Aroma Detection , 2013, Sensors.

[10]  Matthias Heinemann,et al.  Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis. , 2011, Nature chemical biology.

[11]  E. Andrianantoandro,et al.  Synthetic biology: new engineering rules for an emerging discipline , 2006, Molecular systems biology.

[12]  Pawel L. Urban,et al.  Open-Source Electronics As a Technological Aid in Chemical Education , 2014 .

[13]  Dong-Myung Kim,et al.  Prolonging cell-free protein synthesis with a novel ATP regeneration system. , 1999, Biotechnology and bioengineering.

[14]  Ahmad S. Khalil,et al.  Synthetic biology: applications come of age , 2010, Nature Reviews Genetics.

[15]  Karen M Polizzi.  What is synthetic biology? , 2013, Methods in molecular biology.

[16]  D. E. Atkinson,et al.  Adenylate Energy Charge in Escherichia coli During Growth and Starvation , 1971, Journal of bacteriology.

[17]  M. Murtiashaw,et al.  Synthesis of an N-acyl sulfamate analog of luciferyl-AMP: a stable and potent inhibitor of firefly luciferase. , 2005, Bioorganic & medicinal chemistry letters.

[18]  Carl A. Michal.  A low-cost spectrometer for NMR measurements in the Earth's magnetic field , 2010 .

[19]  Christopher P Austin,et al.  Characterization of chemical libraries for luciferase inhibitory activity. , 2008, Journal of medicinal chemistry.