Expression in Acute Myelogenous Leukemia

The retinoblastoma (RB) protein levels in blast-enriched mononuclear fractions from the peripheral blood of 33 newly diagnosed patients with acute myelogenous leukemia were studied. Ten patients who had previously been treated were also analyzed, nine of whom had achieved prior complete remission. Low RB protein expression was found in 13 of 43 (30%) of the acute myelogenous leukemia patients as determined by Western blotting and immunochemical analysis. Of particular interest among the 20 newly diagnosed patients treated with the same therapeu tic regimen, the median survival was 39 days for those with low RB protein expression compared to 333 days for those with high levels of RB protein expression in their leukemic cells (/' 0.02). This prelim inary study suggests that decreases of RB protein expression in periph eral blood of myeloid leukemic cells occur frequently and may be asso ciated with shortened survival of acute myelogenous leukemia patients.

[1]  P. Thall,et al.  Treatment of newly diagnosed acute myelogenous leukemia with granulocyte-macrophage colony-stimulating factor (GM-CSF) before and during continuous-infusion high-dose ara-C + daunorubicin: comparison to patients treated without GM-CSF. , 1992, Blood.

[2]  S. Maeda,et al.  Inactivation of the retinoblastoma gene in human bladder and renal cell carcinomas. , 1991, Cancer research.

[3]  C. Cordon-Cardo,et al.  Molecular genetic alterations in superficial and locally advanced human bladder cancer. , 1991, Cancer research.

[4]  M. Belvin,et al.  Heterogeneous expression of the product of the retinoblastoma susceptibility gene in primary human leukemia cells. , 1991, Oncogene.

[5]  W. Benedict,et al.  Lack of nuclear RB protein staining in G0/middle G1 cells: correlation to changes in total RB protein level. , 1991, Oncogene.

[6]  M. Raffeld,et al.  Inactivation of the retinoblastoma gene in human lymphoid neoplasms. , 1991, Blood.

[7]  C. Cordon-Cardo,et al.  Altered expression of the retinoblastoma gene product in human sarcomas. , 1990, The New England journal of medicine.

[8]  W. Lee,et al.  Suppression of tumorigenicity of human prostate carcinoma cells by replacing a mutated RB gene. , 1990, Science.

[9]  W. Lee,et al.  Homozygous deletion of the retinoblastoma gene in an acute lymphoblastic leukemia (T) cell line. , 1990, Blood.

[10]  E. Estey,et al.  Prediction of survival during induction therapy in patients with newly diagnosed acute myeloblastic leukemia. , 1989, Leukemia.

[11]  J. Varley,et al.  Structural rearrangement of the retinoblastoma gene in human breast carcinoma. , 1988, Science.

[12]  J. Minna,et al.  Abnormalities in structure and expression of the human retinoblastoma gene in SCLC. , 1988, Science.

[13]  K. Ishizaki,et al.  Chromosomal reorganization for the expression of recessive mutation of retinoblastoma susceptibility gene in the development of osteosarcoma. , 1988, Cancer research.

[14]  W. Lee,et al.  Inactivation of the retinoblastoma susceptibility gene in human breast cancers. , 1988, Science.

[15]  A. Cork,et al.  Toward a clinically relevant cytogenetic classification of acute myelogenous leukemia. , 1987, Leukemia research.