[Bacterial endosymbiosis within the cytoplasm of Acanthamoeba lugdunensis isolated from a contact lens storage case].

Transmission electron microscopy of an Acanthamoeba isolate (KA/L5) from a contact lens case revealed bacterial endosymbionts within cytoplasm of the amoebae. The Acanthamoeba isolate belonged to the morphological group II. Based on the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) of 18S ribosomal RNA coding DNA (rDNA), the isolate was identified as A. lugdunensis. Strain typing by isoenzyme analysis using isoelectric focusing (IEF) and mitochondrial (Mt) DNA RFLP revealed that the isolate was closely related with KA/L1, the most predominant type of isolates from contact lens storage cases, KA/E2, a clinical isolate, KA/W4, previously reported to host endosymbionts, and L3a strains of A. lugdunensis. The endosymbionts were similar to those of KA/W4 in aspects that they were randomly distributed in both trophozoites and cysts, and were rod-shaped bacteria measuring approximately 1.38 x 0.50 microns. But the number of endosymbionts per amoeba was significantly lower than that of KA/W4. They were neither limited by phagosomal membranes nor included in lacunaelike structure.

[1]  C. Park,et al.  Intestinal pathologic findings at early stage infection by Centrocestus armatus in albino rats. , 1997, The Korean journal of parasitology.

[2]  H. Kong,et al.  Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga. , 1995, The Korean journal of parasitology.

[3]  T. Fritsche,et al.  Transmissibility of Bacterial Endosymbionts Between Isolates of Acanthamoeba Spp. , 1995, The Journal of eukaryotic microbiology.

[4]  F. Higa,et al.  Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method , 1993, Applied and environmental microbiology.

[5]  T. Fritsche,et al.  Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses , 1993, Journal of clinical microbiology.

[6]  M. Loveless,et al.  Cutaneous acanthamoeba infection associated with leukocytoclastic vasculitis in an AIDS patient. , 1993, The American Journal of dermatopathology.

[7]  B. Drasar,et al.  Association of Vibrio cholerae with fresh water amoebae. , 1992, Journal of medical microbiology.

[8]  B. Fields.  The role of amoebae in Legionellosis , 1991 .

[9]  G. Visvesvara.  Classification of Acanthamoeba. , 1991, Reviews of infectious diseases.

[10]  W. Drozanski.  Sarcobium lyticum gen. nov., sp. nov., an Obligate Intracellular Bacterial Parasite of Small Free-Living Amoebae† , 1991 .

[11]  T. Endo,et al.  Restriction enzyme analysis of mitochondrial DNA of Acanthamoeba strains in Japan. , 1990, The Journal of protozoology.

[12]  H. Müller,et al.  Ingested Listeria monocytogenes survive and multiply in protozoa. , 1990, Journal of medical microbiology.

[13]  H. Müller,et al.  INTERACTIONS OF LISTERIA MONOCYTOGENES, LISTERIA SEELIGERI, AND LISTERIA INNOCUA WITH PROTOZOANS , 1990 .

[14]  J. Hall,et al.  Bacterial endosymbionts of Acanthamoeba sp. , 1985, The Journal of parasitology.

[15]  J. Jonckheere.  Growth characteristics, cytopathic effect in cell culture, and virulence in mice of 36 type strains belonging to 19 different Acanthamoeba spp. , 1980 .

[16]  G. Lupașcu,et al.  Electron microscopic study of a pathogenic Acanthamoeba castellani strain: the presence of bacterial endosymbionts. , 1975, International journal for parasitology.