A highly sensitive and specific capacitive aptasensor for rapid and label-free trace analysis of Bisphenol A (BPA) in canned foods.

A rapid, highly sensitive, specific and low-cost capacitive affinity biosensor is presented here for label-free and single step detection of Bisphenol A (BPA). The sensor design allows rapid prototyping at low-cost using printed circuit board material by benchtop equipment. High sensitivity detection is achieved through the use of a BPA-specific aptamer as probe molecule and large electrodes to enhance AC-electroelectrothermal effect for long-range transport of BPA molecules toward electrode surface. Capacitive sensing technique is used to determine the bounded BPA level by measuring the sample/electrode interfacial capacitance of the sensor. The developed biosensor can detect BPA level in 20s and exhibits a large linear range from 1 fM to 10 pM, with a limit of detection (LOD) of 152.93 aM. This biosensor was applied to test BPA in canned food samples and could successfully recover the levels of spiked BPA. This sensor technology is demonstrated to be highly promising and reliable for rapid, sensitive and on-site monitoring of BPA in food samples.

[1]  Q. Yuan,et al.  Optimization of planar interdigitated microelectrode array for biofluid transport by AC electrothermal effect , 2014 .

[2]  Hongyuan Jiang,et al.  An AC electrokinetic impedance immunosensor for rapid detection of tuberculosis. , 2013, The Analyst.

[3]  Lei Zheng,et al.  Electrochemical aptasensor for the determination of bisphenol A in drinking water , 2012, Microchimica Acta.

[4]  Bo-Hao Wu,et al.  Reusable electrochemical sensor for bisphenol A based on ionic liquid functionalized conducting polymer platform. , 2016, Talanta.

[5]  J. Kozak,et al.  Tyrosinase-based biosensor for determination of bisphenol A in a flow-batch system. , 2015, Talanta.

[6]  Cheng Cheng,et al.  Rapid detection of progesterone by commercially available microelectrode chips , 2013, 2013 IEEE SENSORS.

[7]  Hongyuan Jiang,et al.  AC electrokinetics-enhanced capacitive immunosensor for point-of-care serodiagnosis of infectious diseases. , 2014, Biosensors & bioelectronics.

[8]  Mariana F. Fernández,et al.  Bisphenol A: Human exposure and neurobehavior. , 2015, Neurotoxicology.

[9]  Lili Zhu,et al.  Electrochemical sensor based on magnetic molecularly imprinted nanoparticles at surfactant modified magnetic electrode for determination of bisphenol A. , 2014, Biosensors & bioelectronics.

[10]  Xianbo Lu,et al.  3D metal-organic framework as highly efficient biosensing platform for ultrasensitive and rapid detection of bisphenol A. , 2014, Biosensors & bioelectronics.

[11]  Shungui Zhou,et al.  Label-free DNA Y junction for bisphenol A monitoring using exonuclease III-based signal protection strategy. , 2016, Biosensors & bioelectronics.

[12]  Chunhai Fan,et al.  Aptamer-based biosensors , 2008 .

[13]  S. Snyder,et al.  HPLC-fluorescence detection and adsorption of bisphenol A, 17beta-estradiol, and 17alpha-ethynyl estradiol on powdered activated carbon. , 2003, Water research.

[14]  H Zhao,et al.  A highly sensitive electrochemical sensor for simultaneous determination of hydroquinone and bisphenol A based on the ultrafine Pd nanoparticle@TiO2 functionalized SiC. , 2014, Analytica chimica acta.

[15]  M. Mirlohi,et al.  Bisphenol A in “BPA free” baby feeding bottles , 2012, Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences.

[16]  Yunqing Liu,et al.  A novel electrochemical aptasensor for bisphenol A assay based on triple-signaling strategy. , 2016, Biosensors & bioelectronics.

[17]  Saurabh Srivastava,et al.  Tyrosinase conjugated reduced graphene oxide based biointerface for bisphenol A sensor. , 2015, Biosensors & bioelectronics.

[18]  Wenyu Wu,et al.  E-assay concept: detection of bisphenol A with a label-free electrochemical competitive immunoassay. , 2014, Biosensors & bioelectronics.

[19]  J. Choo,et al.  Surface-enhanced Raman scattering aptasensor for ultrasensitive trace analysis of bisphenol A. , 2015, Biosensors & bioelectronics.

[20]  J. Wu,et al.  Interactions of electrical fields with fluids: laboratory-on-a-chip applications. , 2008, IET nanobiotechnology.

[21]  T. Begley,et al.  Evaluation of Long-Term Migration Testing from Can Coatings into Food Simulants: Polyester Coatings. , 2016, Journal of agricultural and food chemistry.

[22]  Qing Lv,et al.  Disposable paper-based electrochemical sensor based on stacked gold nanoparticles supported carbon nanotubes for the determination of bisphenol A , 2016 .

[23]  Xiaogang Lin,et al.  Rapid and sensitive detection of small biomolecule by capacitive sensing and low field AC electrothermal effect , 2016 .

[24]  Md Azahar Ali,et al.  Self assembled DC sputtered nanostructured rutile TiO₂ platform for bisphenol A detection. , 2015, Biosensors & bioelectronics.

[25]  Xu-Liang Cao,et al.  Bisphenol a in canned food products from canadian markets. , 2010, Journal of food protection.

[26]  K. Robien,et al.  Bisphenol A exposure and associations with obesity among adults: a critical review , 2014, Public Health Nutrition.

[27]  Guoying Feng,et al.  Bisphenol A Sensors on Polyimide Fabricated by Laser Direct Writing for Onsite River Water Monitoring at Attomolar Concentration. , 2016, ACS applied materials & interfaces.

[28]  Flavourings,et al.  Scientific Opinion on the risks to public health related to the presence of bisphenol A (BPA) in foodstuffs , 2015 .

[29]  K. Brogden.  Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? , 2005, Nature Reviews Microbiology.

[30]  Nicole Jaffrezic-Renault,et al.  Highly sensitive electrochemical biosensor for bisphenol A detection based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled carbon nanotube-tyrosinase hybrid film. , 2015, Biosensors & bioelectronics.

[31]  Jianying Hu,et al.  Determination of alkylphenol and bisphenol A in beverages using liquid chromatography/electrospray ionization tandem mass spectrometry , 2005 .

[32]  A. Goodson,et al.  Survey of bisphenol A and bisphenol F in canned foods , 2002, Food additives and contaminants.

[33]  K. Sasaki,et al.  [Determination of bisphenol A in foods using GC/MS]. , 2001, Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan.

[34]  Dong Hoon Shin,et al.  Ultrasensitive Bisphenol A Field-Effect Transistor Sensor Using an Aptamer-Modified Multichannel Carbon Nanofiber Transducer. , 2016, ACS applied materials & interfaces.

[35]  Qiang Wang,et al.  Aptamer-functionalized nanoporous gold film for high-performance direct electrochemical detection of bisphenol A in human serum. , 2015, Analytica chimica acta.

[36]  Liguang Xu,et al.  Building an aptamer/graphene oxide FRET biosensor for one-step detection of bisphenol A. , 2015, ACS applied materials & interfaces.

[37]  Dan M Close,et al.  Rapid detection of microbial cell abundance in aquatic systems. , 2016, Biosensors & bioelectronics.

[38]  Daodong Pan,et al.  Functional graphene-gold nano-composite fabricated electrochemical biosensor for direct and rapid detection of bisphenol A. , 2015, Analytica chimica acta.

[39]  H. Nohta,et al.  Liquid chromatographic determination of bisphenols based on intramolecular excimer-forming fluorescence derivatization , 2003 .

[40]  I. Hayasaka,et al.  Toxicokinetics of bisphenol A in rats, monkeys and chimpanzees by the LC-MS/MS method. , 2006, Toxicology.

[41]  Kwang Su Kim,et al.  Electrochemical detection of Bisphenol A with high sensitivity and selectivity using recombinant protein-immobilized graphene electrodes. , 2015, Biosensors & bioelectronics.

[42]  Jun Zhang,et al.  Bisphenol-A and Female Infertility: A Possible Role of Gene-Environment Interactions , 2015, International journal of environmental research and public health.

[43]  E. N. Aghdam,et al.  Design and characterization of a passive, disposable wireless AC-electroosmotic lab-on-a-film for particle and fluid manipulation , 2016 .

[44]  Liguang Xu,et al.  Building SERS-active heteroassemblies for ultrasensitive Bisphenol A detection. , 2016, Biosensors & bioelectronics.

[45]  A. Bratov,et al.  Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria. , 2016, Biosensors & bioelectronics.

[46]  Lei Zheng,et al.  Rapid capacitive detection of femtomolar levels of bisphenol A using an aptamer-modified disposable microelectrode array , 2015, Microchimica Acta.

[47]  Xinsheng Liu,et al.  Electrochemical determination of bisphenol A at ordered mesoporous carbon modified nano-carbon ionic liquid paste electrode. , 2016, Talanta.

[48]  Jiehong Wu,et al.  Micropumping of biofluids by alternating current electrothermal effects , 2007 .

[49]  Hsueh-Chia Chang,et al.  Particle detection by electrical impedance spectroscopy with asymmetric-polarization AC electroosmotic trapping , 2005 .

[50]  M. Lian,et al.  AC electrothermal manipulation of conductive fluids and particles for lab-chip applications. , 2007, IET nanobiotechnology.

[51]  P. Hunt,et al.  An old culprit but a new story: bisphenol A and "NextGen" bisphenols. , 2016, Fertility and sterility.

[52]  Yanbin Li,et al.  An electrochemical aptasensor based on gold nanoparticles dotted graphene modified glassy carbon electrode for label-free detection of bisphenol A in milk samples. , 2014, Food chemistry.

[53]  Zhouping Wang,et al.  Mn2+-doped NaYF4:Yb/Er upconversion nanoparticle-based electrochemiluminescent aptasensor for bisphenol A , 2016, Analytical and Bioanalytical Chemistry.

[54]  Jing Jin,et al.  Nanographene-based tyrosinase biosensor for rapid detection of bisphenol A. , 2012, Biosensors & bioelectronics.

[55]  Yu Cao,et al.  Sensitivity and selectivity determination of bisphenol A using SWCNT-CD conjugate modified glassy carbon electrode. , 2012, Journal of hazardous materials.

[56]  Taisen Iguchi,et al.  An ecological assessment of bisphenol-A: evidence from comparative biology. , 2007, Reproductive toxicology.