Characterization of a recombinant granzyme B derivative as a "restriction" protease.

[1]  M. Etzerodt,et al.  Expression, refolding, and purification of recombinant human granzyme B. , 2005, Protein expression and purification.

[2]  R. Fletterick,et al.  Characterization of Structural Determinants of Granzyme B Reveals Potent Mediators of Extended Substrate Specificity* , 2004, Journal of Biological Chemistry.

[3]  R. Sitia,et al.  Stress, Protein (Mis)folding, and Signaling: The Redox Connection , 2004, Science's STKE.

[4]  K. Mann,et al.  A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. , 2003, Protein expression and purification.

[5]  G. Korbutt,et al.  Granzyme B: a natural born killer , 2003, Immunological reviews.

[6]  I. Goping,et al.  Mitochondria at the heart of the cytotoxic attack. , 2003, Biochemical and biophysical research communications.

[7]  J. Whisstock,et al.  Determination of the P1', P2' and P3' subsite-specificity of factor Xa. , 2003, The international journal of biochemistry & cell biology.

[8]  J. Powers,et al.  Dipeptidyl peptidase I: importance of progranzyme activation sequences, other dipeptide sequences, and the N-terminal amino group of synthetic substrates for enzyme activity. , 2002, Archives of biochemistry and biophysics.

[9]  L. Juliano,et al.  Mapping of the Catalytic Groove Preferences of Factor Xa Reveals an Inadequate Selectivity for Its Macromolecule Substrates* , 2002, The Journal of Biological Chemistry.

[10]  J. Trapani Granzymes: a family of lymphocyte granule serine proteases , 2001, Genome Biology.

[11]  J. Whisstock,et al.  Importance of the P4′ Residue in Human Granzyme B Inhibitors and Substrates Revealed by Scanning Mutagenesis of the Proteinase Inhibitor 9 Reactive Center Loop* , 2001, The Journal of Biological Chemistry.

[12]  H G Bull,et al.  The three-dimensional structure of human granzyme B compared to caspase-3, key mediators of cell death with cleavage specificity for aspartic acid in P1. , 2001, Chemistry & biology.

[13]  R. Huber,et al.  Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue , 2000, Biological chemistry.

[14]  Robert Fletterick,et al.  The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity , 2000, Nature Structural Biology.

[15]  J. Lieberman,et al.  Mitochondria-dependent and -independent Regulation of Granzyme B–induced Apoptosis , 1999, The Journal of experimental medicine.

[16]  Jennifer L. Harris,et al.  Definition and Redesign of the Extended Substrate Specificity of Granzyme B* , 1998, The Journal of Biological Chemistry.

[17]  L. Briand,et al.  How the substitution of K188 of trypsin binding site by aromatic amino acids can influence the processing of beta-casein. , 1998, Biochemical and biophysical research communications.

[18]  N. Thornberry,et al.  A Combinatorial Approach Defines Specificities of Members of the Caspase Family and Granzyme B , 1997, The Journal of Biological Chemistry.

[19]  J. Trapani,et al.  Granzymes: exogenous proteinases that induce target cell apoptosis. , 1995, Immunology today.

[20]  S. Moestrup,et al.  Purified alpha 2-macroglobulin receptor/LDL receptor-related protein binds urokinase.plasminogen activator inhibitor type-1 complex. Evidence that the alpha 2-macroglobulin receptor mediates cellular degradation of urokinase receptor-bound complexes. , 1992, The Journal of biological chemistry.

[21]  J. Tschopp,et al.  Human and murine cytotoxic T lymphocyte serine proteases: subsite mapping with peptide thioester substrates and inhibition of enzyme activity and cytolysis by isocoumarins. , 1991, Biochemistry.

[22]  N H Sigal,et al.  Human cytotoxic lymphocyte granzyme B. Its purification from granules and the characterization of substrate and inhibitor specificity. , 1991, The Journal of biological chemistry.

[23]  P. Peters,et al.  Interaction of chondroitin sulfate with perforin and granzymes of cytolytic T-cells is dependent on pH. , 1990, Biochemistry.

[24]  F. Studier,et al.  Use of T7 RNA polymerase to direct expression of cloned genes. , 1990, Methods in enzymology.

[25]  P. Coleman,et al.  Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates. , 1981, Methods in enzymology.