β1‐integrin protects hepatoma cells from chemotherapy induced apoptosis via a mitogen‐activated protein kinase dependent pathway

β1‐integrin modulates cellular phenotype by mediating signals from the extracellular matrix (ECM). Although overexpression of integrin molecules in hepatocellular carcinoma (HCC) has been reported, the role of overexpressed β1‐integrin in the disease process of HCC is not fully understood. The authors investigated the effects of β1‐integrin on apoptosis in hepatoma cells.

[1]  R. Juliano The role of beta 1 integrins in tumors. , 1993, Seminars in cancer biology.

[2]  pierre-Marie Martin,et al.  Up‐regulation of α2β1 integrin cell‐surface expresssion protects A431 cells from epidermal growth factor–induced apoptosis , 2000 .

[3]  G. Viale,et al.  Patterns of integrin common chain β1 and collagen IV immunoreactivity in hepatocellular carcinoma. Correlations with tumour growth rate, grade and size , 1993, The Journal of pathology.

[4]  John Calvin Reed,et al.  The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates Bcl-2 expression. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[5]  Asim Khwaja,et al.  Matrix adhesion and Ras transformation both activate a phosphoinositide 3‐OH kinase and protein kinase B/Akt cellular survival pathway , 1997, The EMBO journal.

[6]  S. Cosulich,et al.  The role of protein kinase B and mitogen‐activated protein kinase in epidermal growth factor and tumor necrosis factor α–mediated rat hepatocyte survival and apoptosis , 2000, Hepatology.

[7]  T. Kao,et al.  Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. , 1985, Nucleic acids research.

[8]  V. Desmet,et al.  Integrins as differential cell lineage markers of primary liver tumors. , 1993, The American journal of pathology.

[9]  T. Ueno,et al.  Integrin alpha6beta1 plays a significant role in the attachment of hepatoma cells to laminin. , 1999, Journal of hepatology.

[10]  M. Neuman Apoptosis in Diseases of the Liver , 2001, Critical reviews in clinical laboratory sciences.

[11]  K. Okita,et al.  Fibrosis accelerates the development of enzyme‐altered lesions in the rat liver , 1998, Hepatology.

[12]  A. Mercurio,et al.  Knockout of alpha6 beta1-integrin expression reverses the transformed phenotype of hepatocarcinoma cells. , 1998, Gastroenterology.

[13]  G. Toda,et al.  Serum levels of integrins in chronic liver diseases. , 1994, Pathology, research and practice.

[14]  T. Ishikawa,et al.  Immunohistochemical study of expression and cellular localization of the multidrug resistance gene product p‐glycoprotein in primary liver carcinoma , 1994, Cancer.

[15]  C. Chi,et al.  Comparison of 2‐methoxyestradiol‐induced, docetaxel‐induced, and paclitaxel‐induced apoptosis in hepatoma cells and its correlation with reactive oxygen species , 2000, Cancer.

[16]  E. Hirsch,et al.  Impaired migration but not differentiation of haematopoietic stem cells in the absence of β1 integrins , 1996, Nature.

[17]  J. Cullen,et al.  Overexpression of a p‐glycoprotein in hepatocellular carcinomas from woodchuck hepatitis virus‐infected woodchucks (Marmota monax) , 1996, Hepatology.

[18]  Kyosuke Yamamoto,et al.  Involvement of the Ets-1 gene in overexpression of matrilysin in human hepatocellular carcinoma. , 2000, Cancer research.

[19]  F. Berditchevski,et al.  Association of TM4SF proteins with integrins: relevance to cancer. , 1996, Biochimica et biophysica acta.

[20]  R. Assoian,et al.  Integrin-dependent activation of MAP kinase: a link to shape-dependent cell proliferation. , 1995, Molecular biology of the cell.

[21]  B. Wiedenmann,et al.  A novel function for the tumor suppressor p16(INK4a): induction of anoikis via upregulation of the alpha(5)beta(1) fibronectin receptor. , 2000, The Journal of cell biology.

[22]  C. Bloch,et al.  Bcl-2 inhibits chemotherapy-induced apoptosis in neuroblastoma. , 1994, Cancer research.

[23]  Y. Takada,et al.  Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies. , 1993, The Journal of biological chemistry.

[24]  M. Minemura,et al.  Overexpression of multidrug resistance genes MDR1 and cMOAT in human hepatocellular carcinoma and hepatoblastoma cell lines. , 1999, International journal of oncology.

[25]  Christopher Haslett,et al.  Extracellular matrix proteins protect small cell lung cancer cells against apoptosis: A mechanism for small cell lung cancer growth and drug resistance in vivo , 1999, Nature Medicine.

[26]  K. Shiraki,et al.  Hepatocyte growth factor promotes cell survival from Fas‐mediated cell death in hepatocellular carcinoma cells via Akt activation and Fas‐death–inducing signaling complex suppression , 2000, Hepatology.

[27]  M. Gottesman Multidrug resistance during chemical carcinogenesis: a mechanism revealed? , 1988, Journal of the National Cancer Institute.

[28]  P. Galle,et al.  Drug-induced apoptosis in hepatoma cells is mediated by the CD95 (APO-1/Fas) receptor/ligand system and involves activation of wild-type p53. , 1997, The Journal of clinical investigation.

[29]  A. Mercurio,et al.  Knockout of α6β1-integrin expression reverses the transformed phenotype of hepatocarcinoma cells , 1998 .

[30]  G. Caridi,et al.  Induction of apoptosis in human neuroblastoma cells by abrogation of integrin‐mediated cell adhesion , 1997, International journal of cancer.

[31]  J. Hickman,et al.  Apoptosis induced by anticancer drugs , 1992, Cancer and Metastasis Reviews.

[32]  K. Tanikawa,et al.  Integrin α6β1 plays a significant role in the attachment of hepatoma cells to laminin , 1999 .

[33]  Jeffrey W. Clark,et al.  A phase I study of gemcitabine and docetaxel in patients with metastatic solid tumors , 2000, Cancer.

[34]  Richard O. Hynes,et al.  Integrins: Versatility, modulation, and signaling in cell adhesion , 1992, Cell.

[35]  H. Aikata,et al.  Long-term prognosis of patients undergoing transcatheter arterial chemoembolization for unresectable hepatocellular carcinoma: comparison of cisplatin lipiodol suspension and doxorubicin hydrochloride emulsion. , 2001, Journal of vascular and interventional radiology : JVIR.

[36]  H. Blau,et al.  Differentiation requires continuous regulation , 1991, The Journal of cell biology.

[37]  P. Johnson,et al.  Systemic chemotherapy of liver tumors. , 2000, Seminars in surgical oncology.

[38]  C. Botta,et al.  A human integrin beta 1 subunit with a unique cytoplasmic domain generated by alternative mRNA processing. , 1990, Gene.

[39]  A. Suzuki,et al.  Mitochondrial Regulation of Cell Death: Mitochondria Are Essential for Procaspase 3-p21 Complex Formation To Resist Fas-Mediated Cell Death , 1999, Molecular and Cellular Biology.

[40]  K. Takenaka,et al.  Differential display and integrin alpha 6 messenger RNA overexpression in hepatocellular carcinoma , 1995, Hepatology.

[41]  John Calvin Reed,et al.  Anchorage dependence, integrins, and apoptosis , 1994, Cell.

[42]  B. Wiedenmann,et al.  A Novel Function for the Tumor Suppressor p16INK4a , 2000, The Journal of Cell Biology.

[43]  Ding‐Shinn Chen,et al.  Chronic oral etoposide and tamoxifen in the treatment of far‐advanced hepatocellular carcinoma , 1996, Cancer.

[44]  A. Masumoto,et al.  Role of β1 integrins in adhesion and invasion of hepatocellular carcinoma cells , 1999 .

[45]  M. Kuo,et al.  Interleukin-6 Inhibits Transforming Growth Factor-β-induced Apoptosis through the Phosphatidylinositol 3-Kinase/Akt and Signal Transducers and Activators of Transcription 3 Pathways* , 1999, The Journal of Biological Chemistry.

[46]  M. Ishiyama,et al.  A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. , 1996, Biological & pharmaceutical bulletin.

[47]  R. Mannix,et al.  Integrin activation suppresses etoposide-induced DNA strand breakage in cultured murine tumor-derived endothelial cells. , 1996, Cancer research.

[48]  M. Colombo,et al.  Hepatocellular carcinoma in patients with HCV. , 2000, Bailliere's best practice & research. Clinical gastroenterology.

[49]  K. Yamamoto,et al.  Differential expression of laminin receptors in human hepatocellular carcinoma , 1998, Gut.

[50]  Z. Werb,et al.  Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix , 1995, Science.

[51]  S. Dedhar,et al.  Integrin cytoplasmic interactions and bidirectional transmembrane signalling. , 1996, Current opinion in cell biology.