Micro patterned surfaces allow long-term digital holographic microscopy live cell imaging

During long-term imaging, cells move out of the field of view. We have generated functionalized substrates containing rectangular areas, which were capable in keeping cells over the whole observation period.

[1]  Sarah Mues,et al.  Optical analysis of nanomaterial-cell interactions: flow cytometry and digital holographic microscopy , 2015, Optical Metrology.

[2]  B. Kemper,et al.  Digital holographic microscopy for live cell applications and technical inspection. , 2008, Applied optics.

[3]  Björn Kemper,et al.  Multimodal optical phenotyping of cancer cells , 2015, Photonics West - Biomedical Optics.

[4]  Steffi Ketelhut,et al.  Multimodal label-free in vitro toxicity testing with digital holographic microscopy , 2014, Photonics Europe.

[5]  H. Schönherr,et al.  Control of Cell Attachment and Spreading on Poly(acrylamide) Brushes with Varied Grafting Density. , 2016, Langmuir : the ACS journal of surfaces and colloids.

[6]  Steffi Ketelhut,et al.  Continuous morphology and growth monitoring of different cell types in a single culture using quantitative phase microscopy , 2015, Optical Metrology.

[7]  Steve Edmondson,et al.  Polymer Brushes via Surface‐Initiated Polymerizations , 2004 .

[8]  Sarah Mues,et al.  Novel optical approaches for label-free quantification of nano-cytotoxic effects , 2016, SPIE BiOS.

[9]  E. Cuche,et al.  Digital holography for quantitative phase-contrast imaging. , 1999, Optics letters.

[10]  R. Tampé,et al.  Protein resistant oligo(ethylene glycol) terminated self-assembled monolayers of thiols on gold by vapor deposition in vacuum , 2010, Biointerphases.

[11]  D E Ingber,et al.  Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver. , 1997, Experimental cell research.

[12]  Milan Mrksich,et al.  Self-assembled monolayers of alkanethiolates presenting mannitol groups are inert to protein adsorption and cell attachment , 2000 .

[13]  Patrik Langehanenberg,et al.  Dynamic in vivo analysis of drug induced actin cytoskeleton degradation by digital holographic microscopy , 2007, European Conference on Biomedical Optics.

[14]  Steffi Ketelhut,et al.  Multimodal label-free growth and morphology characterization of different cell types in a single culture with quantitative digital holographic phase microscopy , 2015, Photonics West - Biomedical Optics.