Collimated superfine x-ray beam based x-ray luminescence computed tomography.

X-ray luminescence computed tomography (XLCT) is a hybrid imaging modality with the potential to achieve a spatial resolution up to several hundred micrometers for targets embedded in turbid media with a depth larger than several millimeters. In this paper, we report a high spatial resolution XLCT imaging system with a collimated superfine x-ray beam in imaging the deeply embedded targets. A collimator with a 100 micrometer pinhole was mounted in the front of a powerful x-ray tube to generate a superfine x-ray pencil beam with a beam diameter of 0.175 mm. For the phantom experiment of four capillary targets with an edge-to-edge distance of 400 micrometers, we were able to reconstruct the targets in a depth of 5 mm successfully, which were validated with microCT images. We have further investigated the effect of different x-ray beam diameters on the reconstructed XLCT images with numerical simulations. Our results indicate that XLCT has the ability to image successfully multiple deeply embedded targets when the collimated x-ray beam diameter is less than or equal to the target edge-to-edge distance. Our numerical simulations also demonstrate that XLCT can achieve a spatial resolution of 200 micrometers for targets embedded at a depth of 5 mm if the scanning beam has a diameter of 100 micrometers.

[1]  Changqing Li,et al.  Nonconvex regularizations in fluorescence molecular tomography for sparsity enhancement , 2014, Physics in medicine and biology.

[2]  Wei Zhang,et al.  Multiple pinhole collimator based X-ray luminescence computed tomography. , 2016, Biomedical optics express.

[3]  Simon R Cherry,et al.  Numerical simulation of x-ray luminescence optical tomography for small-animal imaging , 2014, Journal of biomedical optics.

[4]  Ge Wang,et al.  X-ray micromodulated luminescence tomography in dual-cone geometry , 2014, Journal of biomedical optics.

[5]  Julien Bec,et al.  X-ray luminescence optical tomography imaging: experimental studies. , 2013, Optics letters.

[6]  W. Marsden.  I and J , 2012 .

[7]  Lei Xing,et al.  X-Ray Luminescence Computed Tomography via Selective Excitation: A Feasibility Study , 2010, IEEE Transactions on Medical Imaging.

[8]  Wei Zhang,et al.  Fiber based fast sparse sampling x-ray luminescence computed tomography , 2017, BiOS.

[9]  Jie Tian,et al.  Cone beam x-ray luminescence computed tomography: a feasibility study. , 2013, Medical physics.

[10]  Lihong V. Wang,et al.  Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging , 2006, Nature Biotechnology.

[11]  Aniouar Bjeoumikhov,et al.  Spatial resolution of confocal XRF technique using capillary optics , 2013, Nanoscale Research Letters.

[12]  Lei Xing,et al.  Tomographic molecular imaging of x-ray-excitable nanoparticles. , 2010, Optics letters.

[13]  George J. Havrilla,et al.  SPECIMEN PREPARATION LIMITATIONS IN TRACE ELEMENT ANALYSIS QUANTIFICATION USING MICRO-X-RAY FLUORESCENCE , 2000 .

[14]  Qimei Liao,et al.  In vivo x-ray luminescence tomographic imaging with single-view data. , 2013, Optics letters.

[15]  Wei Zhang,et al.  Microscopic x-ray luminescence computed tomography , 2015, Photonics West - Biomedical Optics.

[16]  Changqing Li,et al.  Nonuniform update for sparse target recovery in fluorescence molecular tomography accelerated by ordered subsets. , 2014, Biomedical optics express.

[17]  Wei Zhang,et al.  Sensitivity study of x-ray luminescence computed tomography. , 2017, Applied optics.