Quantitative phase imaging for enhanced assessment of optomechanical cancer cell properties

Optical cell stretching provides label-free investigations of cells by measuring their biomechanical properties based on deformability determination in a fiber optical two-beam trap. However, the stretching forces in this two-beam laser trap depend on the optical properties of the investigated specimen. Therefore, we characterized in parallel four cancer cell lines with varying degree of differentiation utilizing quantitative phase imaging (QPI) and optical cell stretching. The QPI data allowed enhanced assessment of the mechanical cell properties measured with the optical cell stretcher and demonstrates the high potential of cell phenotyping when both techniques are combined.

[1]  G. Popescu.  Quantitative Phase Imaging of Cells and Tissues , 2011 .

[2]  Daniel Carl,et al.  Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells , 2006, SPIE Photonics Europe.

[3]  P. H. Yap,et al.  Cell refractive index for cell biology and disease diagnosis: past, present and future. , 2016, Lab on a chip.

[4]  H. Elsässer,et al.  Establishment and characterisation of two cell lines with different grade of differentiation derived from one primary human pancreatic adenocarcinoma , 1992, Virchows Archiv. B, Cell pathology including molecular pathology.

[5]  Jochen Guck,et al.  Viscoelastic Properties of Differentiating Blood Cells Are Fate- and Function-Dependent , 2012, PloS one.

[6]  Daniel Carl,et al.  Investigation of living pancreas tumor cells by digital holographic microscopy. , 2006, Journal of biomedical optics.

[7]  M. Lerch,et al.  Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model , 2009, BMC Cancer.

[8]  YoungJu Jo,et al.  Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications , 2013, Sensors.

[9]  Björn Kemper,et al.  Biophysical monitoring of cell cultures for quality assessment utilizing digital holographic microscopy , 2017, Optical Metrology.

[10]  Patrik Langehanenberg,et al.  Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy. , 2007, Journal of biomedical optics.

[11]  J. Käs,et al.  The optical stretcher: a novel laser tool to micromanipulate cells. , 2001, Biophysical journal.

[12]  Stefan Schinkinger,et al.  Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. , 2005, Biophysical journal.

[13]  Patrik Langehanenberg,et al.  Autofocusing in digital holographic microscopy , 2011 .

[14]  B. Kemper,et al.  Digital holographic microscopy for live cell applications and technical inspection. , 2008, Applied optics.

[15]  Stefan Schinkinger,et al.  Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications , 2007, Biomedical microdevices.