Antimicrobial efficacy assessment of multi-use solution to disinfect hydrophilic contact lens, in vitro.

PURPOSE To evaluate the efficacy of disinfecting solutions in hydrophilic contact lenses (CL). METHODS Two multi-use solutions denominated solution A (0.001% polyquaternium-1 and 0.0005% myristamidopropyl dimethylamine) and solution B (0.0001% polyaminopropyl biguanide) were used. The solutions were tested in hydrophilic contact lenses infected with Pseudomonas aeruginosa (ATCC27583), Staphylococcus epidermidis (ATCC1226), Klebsiella pneumoniae (ATCC13883), Staphylococcus aureus (ATCC25923) and Candida albicans (ATCC 10231) and the decrease in microorganisms growth after the hydrophilic contact lenses were cleaned with the respective solutions was verified. The manufacture's instructions were followed. RESULTS A decrease of 90% of Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus, Candida albicans and a decrease 100% of Klebsiella pneumoniae was observed. CONCLUSION The solutions decreased the amount of microorganisms tested.

[1]  D. Meadows,et al.  Biocide Uptake in Contact Lenses and Loss of Fungicidal Activity During Storage of Contact Lenses , 2006, Eye & contact lens.

[2]  H. C. van der Mei,et al.  Influence of Day and Night Wear on Surface Properties of Silicone Hydrogel Contact Lenses and Bacterial Adhesion , 2006, Cornea.

[3]  P. Caroline,et al.  Bacterial Populations on Silicone Hydrogel and Hydrogel Contact Lenses after Swimming in a Chlorinated Pool , 2005, Optometry and vision science : official publication of the American Academy of Optometry.

[4]  H. K. Dannelly,et al.  Effectiveness of Contact Lens Disinfectants After Lens Storage , 2004, Eye & contact lens.

[5]  J. Maillard,et al.  Aspects of the antimicrobial mechanisms of action of a polyquaternium and an amidoamine. , 2003, The Journal of antimicrobial chemotherapy.

[6]  Michael S. Gilmore,et al.  Bacterial Endophthalmitis: Epidemiology, Therapeutics, and Bacterium-Host Interactions , 2002, Clinical Microbiology Reviews.

[7]  Ruth A Rosenthal,et al.  Review of standard for evaluating the effectiveness of contact lens disinfectants. , 2002, PDA journal of pharmaceutical science and technology.

[8]  E. Cohen.  Microbial Colonization of Soft Contact Lenses Over Time , 2001 .

[9]  S. Kilvington,et al.  Comparison of Hydrogen Peroxide Contact Lens Disinfection Systems and Solutions against Acanthamoeba polyphaga , 2001, Antimicrobial Agents and Chemotherapy.

[10]  S. Klotz,et al.  Fungal and Parasitic Infections of the Eye , 2000, Clinical Microbiology Reviews.

[11]  J. Walker,et al.  Efficacy of a contact lens cleaning device and its enhancement of the performance of contact lens care products , 2000, The British journal of ophthalmology.

[12]  M. Joly-Guillou,et al.  Control of Staphylococcal Adhesion to Polymethylmethacrylate and Enhancement of Susceptibility to Antibiotics by Poloxamer 407 , 2000, Antimicrobial Agents and Chemotherapy.

[13]  A. D. Russell,et al.  Antiseptics and Disinfectants: Activity, Action, and Resistance , 2001, Clinical Microbiology Reviews.

[14]  Cowell,et al.  A relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses , 1998, Journal of applied microbiology.

[15]  Stapleton,et al.  Bacterial biofilm on contact lenses and lens storage cases in wearers with microbial keratitis , 1998, Journal of applied microbiology.

[16]  L. Gram,et al.  Enzymatic removal and disinfection of bacterial biofilms , 1997, Applied and environmental microbiology.

[17]  B. Holden,et al.  Microbial contamination of hydrogel contact lenses , 1997, Journal of applied microbiology.

[18]  L. Wilson,et al.  Adaptation and growth of Serratia marcescens in contact lens disinfectant solutions containing chlorhexidine gluconate , 1993, Applied and environmental microbiology.

[19]  N. Efron,et al.  Microbial flora in eyes of current and former contact lens wearers , 1992, Journal of clinical microbiology.

[20]  A. Kanpolat,et al.  Contamination in contact lens care systems. , 1992, The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc.

[21]  S. Sutton,et al.  D-value determinations are an inappropriate measure of disinfecting activity of common contact lens disinfecting solutions , 1991, Applied and environmental microbiology.

[22]  E. C. Poggio,et al.  The incidence of ulcerative keratitis among users of daily-wear and extended-wear soft contact lenses. , 1989, The New England journal of medicine.

[23]  L A Wilson,et al.  Effects of protein, mucin, and human tears on adherence of Pseudomonas aeruginosa to hydrophilic contact lenses , 1988, Journal of clinical microbiology.

[24]  J. Costerton,et al.  Bacterial biofilms in nature and disease. , 1987, Annual review of microbiology.